Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,962,531 G→C 7.3% D70H (GAC→CAC)  dapF → diaminopimelate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,962,5310GC 7.3% 161.0 / 4.0 55D70H (GAC→CAC) dapFdiaminopimelate epimerase
Reads supporting (aligned to +/- strand):  ref base G (29/22);  new base C (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

CTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGCATCATCGTGCT  >  NC_002947/5962387‑5962679
                                                                                                                                                |                                                                                                                                                    
ctgggcAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACtt                                                                                                                                                  >  6:241779/1‑149 (MQ=255)
 tgggcAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTc                                                                                                                                                 >  6:241976/1‑149 (MQ=255)
   ggcAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCg                                                                                                                                               >  2:213096/1‑149 (MQ=255)
    gcAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTCCACTTCCgg                                                                                                                                              <  3:7242/149‑2 (MQ=255)
     cAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGc                                                                                                                                              >  4:315369/1‑148 (MQ=255)
     cAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGc                                                                                                                                              >  6:120453/1‑148 (MQ=255)
      aaCGAGTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGACGGCTTCGACCAGTAGCTGATCGACGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTa                                                                                                                                            >  7:294432/1‑149 (MQ=255)
      aaCGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTa                                                                                                                                            <  1:213096/149‑1 (MQ=255)
      aaCGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTCCACTTCCgggt                                                                                                                                            <  3:133676/149‑4 (MQ=255)
      aaCGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTCCACGTCCgggt                                                                                                                                            >  4:133676/1‑146 (MQ=255)
            ttCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCAt                                                                                                                                      >  6:109488/1‑149 (MQ=255)
               aTGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCtt                                                                                                                                   >  1:147745/1‑149 (MQ=255)
                 ggTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTc                                                                                                                                  >  4:200355/1‑148 (MQ=255)
                  gTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCaa                                                                                                                                >  2:214156/1‑149 (MQ=255)
                        gACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGa                                                                                                                          <  5:152610/149‑1 (MQ=255)
                           cTGGTCAGCCAGCACGCGCACATACAGCCAACGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCACTTGCTGATACTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACCCCCACgg                                                                                                                       >  3:265188/1‑149 (MQ=255)
                              gTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTc                                                                                                                    >  1:286073/1‑149 (MQ=255)
                                        aCGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAg                                                                                                          <  5:241976/149‑1 (MQ=255)
                                          gcgcACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGCACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGc                                                                                                         >  7:86114/1‑148 (MQ=255)
                                            gcACATCCAGCACAAGCACGCCAAGCAATGGGGTGACCGCCATACCGCTGTCTGATTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAAt                                                                                                      >  4:210735/1‑149 (MQ=255)
                                               caTCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCg                                                                                                   <  1:214156/149‑1 (MQ=255)
                                                      cccAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTc                                                                                                             >  4:53109/1‑132 (MQ=255)
                                                      cccAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTc                                                                                                             <  3:53109/132‑1 (MQ=255)
                                                                    gCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTg                                                                              >  1:204949/1‑149 (MQ=255)
                                                                     cAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGc                                                                             <  5:109488/149‑1 (MQ=255)
                                                                       aTGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTcc                                                                                                                                                <  6:233949/80‑1 (MQ=255)
                                                                       aTGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTcc                                                                                                                                                >  5:233949/1‑80 (MQ=255)
                                                                        tGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACCGt                                                                                           >  5:336956/1‑132 (MQ=255)
                                                                         ggggTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGc                                                                         >  4:302922/1‑149 (MQ=255)
                                                                         ggggTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCAACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGc                                                                         >  4:23113/1‑149 (MQ=255)
                                                                          gggTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCt                                                                        <  3:315369/149‑1 (MQ=255)
                                                                               aCCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTg                                                                   >  2:9155/1‑149 (MQ=255)
                                                                                  gCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTg                                                                <  5:241779/149‑1 (MQ=255)
                                                                                  gCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTg                                                                <  3:200355/149‑1 (MQ=255)
                                                                                   ccATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTgg                                                               <  2:286073/149‑1 (MQ=255)
                                                                                       aCCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTgg                                                               <  6:92015/145‑1 (MQ=255)
                                                                                       aCCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTgg                                                               >  5:92015/1‑145 (MQ=255)
                                                                                                       aCCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGaa                                            >  8:291535/1‑148 (MQ=255)
                                                                                                        ccAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTg                                                                   >  5:37103/1‑124 (MQ=255)
                                                                                                        ccAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTg                                                                   <  6:37103/124‑1 (MQ=255)
                                                                                                           gTTGCTGATCGTCGAGGCTCCGAACAACCCGGAAGTGGACTTCCTCTACCTCATCTTCAACGCGGACGGCTCCGAGTTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAgc                                       <  4:265188/149‑1 (MQ=255)
                                                                                                               cTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCAt                                   <  7:291535/149‑1 (MQ=255)
                                                                                                                      tcgAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTg                            >  8:205845/1‑149 (MQ=255)
                                                                                                                      tcgAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTg                            >  6:233025/1‑149 (MQ=255)
                                                                                                                        gAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGa                          <  4:109777/149‑1 (MQ=255)
                                                                                                                                gAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCaa                    >  2:32583/1‑147 (MQ=255)
                                                                                                                                   caacCCGGAAGCGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCCTCCGCGTGGAAACCAAGAGCg               <  8:86114/149‑1 (MQ=255)
                                                                                                                                    aacCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCgg              >  8:221798/1‑149 (MQ=255)
                                                                                                                                    aacACGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCgg              <  8:294432/149‑1 (MQ=255)
                                                                                                                                       gcGGAAGTCCACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGcat           >  2:17613/2‑149 (MQ=255)
                                                                                                                                        cGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGcatc          <  6:160753/149‑1 (MQ=255)
                                                                                                                                           aaGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGCatcatc       <  7:221798/149‑1 (MQ=255)
                                                                                                                                           aaGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTAGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGCatcatc       <  3:210735/149‑1 (MQ=255)
                                                                                                                                             gTGGTCTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAgc                                       <  3:177992/115‑1 (MQ=255)
                                                                                                                                             gTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAgc                                       >  4:177992/1‑115 (MQ=255)
                                                                                                                                                gACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGCATCATCGTGCt  <  2:147745/149‑1 (MQ=255)
                                                                                                                                                gACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCAGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGACACCAAGAGAGGCATAATCGTGCt  >  8:55445/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CTGGGCAACGACTTCATGGTCCTGGACCTGGTCAGCCAGCACGCGCACATCCAGCCCAAGCACGCCAAGCAATGGGGTGACCGCCATACCGGTGTCGGCTTCGACCAGTTGCTGATCGTCGAGGCGCCGAACAACCCGGAAGTGGACTTCCGCTACCGCATCTTCAACGCCGACGGCTCCGAGGTCGAGCAATGCGGCAACGGTGCGCGCTGCTTTGCCCGCTTCGTGCTGGACAAGCGCCTGACCGCGAAAAAGCGCATCCGCGTGGAAACCAAGAGCGGCATCATCGTGCT  >  NC_002947/5962387‑5962679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑