Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,966,333 A→T 5.0% intergenic (‑402/+28) PP_5232 ← / ← amtB hypothetical protein/ammonium transporter AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,966,3330AT 5.0% 292.8 / 2.7 92intergenic (‑402/+28)PP_5232/amtBhypothetical protein/ammonium transporter AmtB
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base T (2/3);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAC  >  NC_002947/5966187‑5966477
                                                                                                                                                  |                                                                                                                                                
ggCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAg                                                                                                                                                 <  6:200951/148‑1 (MQ=255)
 gcgcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGcc                                                                                                                                               >  3:31570/1‑149 (MQ=255)
 gcgcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGcc                                                                                                                                               <  7:61913/149‑1 (MQ=255)
   gcgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCgg                                                                                                                                             >  7:299461/1‑149 (MQ=255)
    cgcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCggg                                                                                                                                            >  4:153444/1‑149 (MQ=255)
     gcATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCggg                                                                                                                                            >  3:295219/1‑148 (MQ=255)
       aTTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGc                                                                                                                                           >  5:122298/1‑147 (MQ=255)
        ttCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCatt                                                                                                                                        >  4:235442/1‑147 (MQ=255)
           tAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttt                                                                                                                                     >  5:24749/1‑149 (MQ=255)
             gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGACTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCACGGCAAGCCGGGCAAGtttt                                                                                                                                    <  1:283836/148‑1 (MQ=255)
             gcgcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGttttt                                                                                                                                   <  3:153444/149‑1 (MQ=255)
               gcgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCATttttttgc                                                                                                                                 <  3:235442/149‑3 (MQ=255)
                cgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCATTTTTTTGcc                                                                                                                                <  7:182742/149‑1 (MQ=255)
                cgcCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTAcc                                                                                                                                <  1:295130/149‑1 (MQ=255)
                   ccGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCAtttttt                                                                                                                                    <  8:766/142‑1 (MQ=255)
                   ccGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCTTGCCGGGCAtttttt                                                                                                                                    >  7:766/1‑142 (MQ=255)
                   ccGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCAGGCAAGTTTTTACcgcg                                                                                                                             >  4:119006/1‑149 (MQ=255)
                        gAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCg                                                                                                                          >  8:305777/1‑147 (MQ=255)
                        gAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCg                                                                                                                          >  6:4013/1‑147 (MQ=255)
                          aaaaGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGAAAGCCGGGCAAGTTTTTACCGCGTCGCAGt                                                                                                                      >  3:172065/1‑149 (MQ=255)
                           aaagcaaagcACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGtt                                                                                                                     <  6:24749/149‑1 (MQ=255)
                             agcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTAc                                                                                                                   <  5:4013/149‑1 (MQ=255)
                              gcaaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACa                                                                                                                  <  3:85206/149‑1 (MQ=255)
                               caaagcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAg                                                                                                                 >  7:125378/1‑149 (MQ=255)
                                  agcaCCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGCCGCAGTTACAGGtt                                                                                                              <  7:276003/149‑1 (MQ=255)
                                      ccATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                                >  2:295162/1‑143 (MQ=255)
                                      ccATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAgg                                                                                                                <  1:295162/143‑1 (MQ=255)
                                       cATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGc                                                                                                         >  1:320969/1‑149 (MQ=255)
                                         tAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcg                                                                                                       <  5:59943/149‑1 (MQ=255)
                                           aaCGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgcg                                                                                                     >  6:186582/1‑149 (MQ=255)
                                              gCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTc                                                                                                  >  8:298557/1‑149 (MQ=255)
                                                 cTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                               <  6:122298/149‑1 (MQ=255)
                                                      gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                          <  7:298557/149‑1 (MQ=255)
                                                      gCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCAGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                          <  3:119006/149‑1 (MQ=255)
                                                        ttGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCt                                                                                          >  7:112750/1‑147 (MQ=255)
                                                        ttGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        <  7:305777/149‑1 (MQ=255)
                                                          gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        <  7:242795/147‑1 (MQ=255)
                                                          gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        <  5:69555/147‑1 (MQ=255)
                                                          gTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        <  1:194137/147‑1 (MQ=255)
                                                           tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        <  5:3982/146‑1 (MQ=255)
                                                           tAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGa                                                                                        >  6:3982/1‑146 (MQ=255)
                                                             aGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAg                                                                                   <  1:11818/149‑1 (MQ=255)
                                                                    tACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                                             <  4:295219/148‑1 (MQ=255)
                                                                     aCCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAgg                                                                            <  2:320969/148‑1 (MQ=255)
                                                                        tACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTAc                                                                                                                                 >  7:133747/1‑92 (MQ=255)
                                                                        tACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTAc                                                                                                                                 <  8:133747/92‑1 (MQ=255)
                                                                          cGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGccgac                                                                       <  8:299461/148‑1 (MQ=255)
                                                                              aCGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGAct                                                                  <  8:112750/149‑1 (MQ=255)
                                                                                 aaCGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtctt                                                               <  5:186582/149‑1 (MQ=255)
                                                                                  aCGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTg                                                                                         <  4:113289/122‑1 (MQ=255)
                                                                                  aCGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTg                                                                                         >  3:113289/1‑122 (MQ=255)
                                                                                  aCGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttc                                                              <  7:182980/149‑1 (MQ=255)
                                                                                    gtgtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACtcttctt                                                            <  1:330525/149‑1 (MQ=255)
                                                                                      gtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGcc                                                                                     >  1:165002/1‑122 (MQ=255)
                                                                                      gtCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGcc                                                                                     <  2:165002/122‑1 (MQ=255)
                                                                                       tCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTcttcttctt                                                         >  5:311726/1‑149 (MQ=255)
                                                                                          aaTAAACCGTTTGCCGTGCGGTAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                      <  4:31570/149‑1 (MQ=255)
                                                                                          aaTAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGt                                                      >  2:323828/1‑149 (MQ=255)
                                                                                              aaCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGAc                                                  >  1:297251/1‑149 (MQ=255)
                                                                                               aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGc                                                                                           <  2:277784/107‑1 (MQ=255)
                                                                                               aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                                             <  7:93568/121‑1 (MQ=255)
                                                                                               aCCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAg                                                                             >  8:93568/1‑121 (MQ=255)
                                                                                                      gCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                        <  2:105110/135‑1 (MQ=255)
                                                                                                      gCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACACGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCttcttcttc                                                        >  1:105110/1‑135 (MQ=255)
                                                                                                       ccGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGcc                                         >  7:46093/1‑149 (MQ=255)
                                                                                                       ccGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGc                                          <  6:311726/148‑1 (MQ=255)
                                                                                                          tGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgcg                                                                                                     <  7:108655/86‑1 (MQ=255)
                                                                                                          tGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgcg                                                                                                     >  8:108655/1‑86 (MQ=255)
                                                                                                          tGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCAt                                      >  4:203549/1‑149 (MQ=255)
                                                                                                              ggAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTTAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATcacc                                  <  8:46093/149‑1 (MQ=255)
                                                                                                               gAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATcacca                                 >  6:325733/1‑149 (MQ=255)
                                                                                                               gAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATcacca                                 >  2:216912/1‑149 (MQ=255)
                                                                                                                 aaTGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACcaca                               >  5:161075/1‑149 (MQ=255)
                                                                                                                     cTGAAAAACTGGCAAAAATATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCa                           <  8:125378/149‑1 (MQ=255)
                                                                                                                      tGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgc                                                                                                      <  5:69107/73‑1 (MQ=255)
                                                                                                                      tGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCcgc                                                                                                      >  6:69107/1‑73 (MQ=255)
                                                                                                                      tGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAg                          >  2:118889/1‑149 (MQ=255)
                                                                                                                      tGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGAGTCGCAGTTACAGGTTGTAGCCGCGCTAGTTGTGCTGAGCCAGGTCGAGGCCGACCGACACTTCTTATTCGTTGACCCGCAGGCCCATCACAACATCCAg                          >  1:273822/1‑149 (MQ=255)
                                                                                                                       gAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAg                          >  6:271391/1‑148 (MQ=255)
                                                                                                                        aaaaaCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGc                         <  3:145888/148‑1 (MQ=255)
                                                                                                                        aaaaaCTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCa                        <  2:31053/149‑1 (MQ=255)
                                                                                                                               ggCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCtt                    >  7:23271/1‑146 (MQ=255)
                                                                                                                                  aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                               >  2:72126/1‑68 (MQ=255)
                                                                                                                                  aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGtt                                                                                               <  1:72126/68‑1 (MQ=255)
                                                                                                                                  aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGaa               >  8:28422/1‑148 (MQ=255)
                                                                                                                                  aaaaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAAt              >  5:53504/1‑149 (MQ=255)
                                                                                                                                    aaaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCa            <  1:323828/149‑1 (MQ=255)
                                                                                                                                     aaaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCAc           <  2:297251/149‑1 (MQ=255)
                                                                                                                                      aaaTGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTGGGAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACg          <  4:172065/149‑1 (MQ=255)
                                                                                                                                         tGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAg       <  7:80672/149‑1 (MQ=255)
                                                                                                                                             cGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGa   >  1:209264/1‑149 (MQ=255)
                                                                                                                                               gCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAc  >  7:164266/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGCGCGCATTCTAGCGCGCCCGTGGAAAAAGCAAAGCACCATAAACGCCCTGCGGCTTGTAAGTTTTTTACCTACGTCACGAACGTGTCGAATAAACCGTTTGCCGTGCGGGAAATGCTGAAAAACTGGCAAAAAAATGCCCGGCAAGCCGGGCAAGTTTTTACCGCGTCGCAGTTACAGGTTGTAGCCGCGCTCGTTGTGCTGAGCCAGGTCGAGGCCGACCGACTCTTCTTCTTCGTTGACCCGCAGGCCCATCACCACATCCAGCACCTTGAGAATCACGTAGGTGAC  >  NC_002947/5966187‑5966477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑