Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,970,304	C→G	6.0%	W330C (TGG→TGC)	PP_5236 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,970,304	0	C	G	6.0%	220.5 / 3.7	67	W330C (TGG→TGC)	PP_5236	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/37);  new base G (2/2);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TCGCGCAGTATCAGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC  >  NC_002947/5970158‑5970445                                                                                                                                                    |                                                                                                                                              tCGCGCAGTATCAGCTCGGCCAAACAGCGAAAGGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCa                                                                                                                                             >  1:324402/1‑149 (MQ=255)   gcgcAGTATCAGCTCGGCCAAACAGCGAAAGGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCAt                                                                                                                                            <  2:324402/148‑1 (MQ=255)    cgcAGTATCAGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGc                                                                                                                                          >  7:223903/1‑149 (MQ=255)      cAGTAACAGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCa                                                                                                                                        <  8:61655/149‑1 (MQ=255)             aGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCTCAGTGCCCCCCGCCATGCCAGAACGAc                                                                                                                                 <  1:182846/149‑1 (MQ=255)             aGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGAc                                                                                                                                 <  2:28486/149‑1 (MQ=255)              gCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGa                                                                                                                                  >  5:172381/1‑147 (MQ=255)               cTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAg                                                                                                                               >  8:231323/1‑149 (MQ=255)                tCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAg                                                                                                                               >  8:297783/1‑148 (MQ=255)                tCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGc                                                                                                                              >  8:204451/1‑149 (MQ=255)                  ggCCAAACAGCGAAAAGTCACGCCCATGGCGCGTATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGc                                                                                                                              <  7:17720/147‑1 (MQ=255)                  ggCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGc                                                                                                                              <  6:172381/147‑1 (MQ=255)                      aaaCAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATATCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTc                                                                                                                        >  2:9769/1‑149 (MQ=255)                      aaaCAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTc                                                                                                                        >  8:165128/1‑149 (MQ=255)                      aaaCAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTc                                                                                                                        >  6:245050/1‑149 (MQ=255)                       aaCAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTcc                                                                                                                       <  3:102763/149‑1 (MQ=255)                           gCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTgg                                                                                                                   <  6:20918/149‑1 (MQ=255)                              aaaaGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGc                                                                                                                              <  5:102289/135‑1 (MQ=255)                              aaaaGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGc                                                                                                                              >  6:102289/1‑135 (MQ=255)                                    cACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACg                                                                                                          >  1:294045/1‑149 (MQ=255)                                         ccATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGt                                                                                                     <  1:303811/149‑1 (MQ=255)                                          cATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGtt                                                                                                    <  2:248084/149‑1 (MQ=255)                                            tGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTtgt                                                                                                  >  4:271190/1‑149 (MQ=255)                                              gcgcGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCTATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGa                                                                                                <  2:71780/149‑1 (MQ=255)                                               cgcgGATGGCGCCGAGATCTGCATTGCACGCCAAGCTCGCGCCAAATTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGAc                                                                                               <  5:159399/149‑1 (MQ=255)                                               cgcgGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGAc                                                                                               >  5:190708/1‑149 (MQ=255)                                               cgcgGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGAc                                                                                               <  5:163937/149‑1 (MQ=255)                                                gcgGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGaa                                                                                                                                     >  1:150880/1‑110 (MQ=255)                                                gcgGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGaa                                                                                                                                     <  2:150880/110‑1 (MQ=255)                                                 cgGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCa                                                                                             <  3:271190/149‑1 (MQ=255)                                                       gcgcCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCtt                                                                                        <  6:190708/148‑1 (MQ=255)                                                       gcgcCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTc                                                                                       <  4:276727/149‑1 (MQ=255)                                                       gcgcCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTc                                                                                       <  1:303287/149‑1 (MQ=255)                                                       gcgcAGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTc                                                                                       <  7:121036/149‑1 (MQ=255)                                                        cgcCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCTCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGTGCGTTGTGACCAGTCTTCg                                                                                      <  4:115132/149‑1 (MQ=255)                                                        cgcCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCg                                                                                      <  2:294045/149‑1 (MQ=255)                                                               aTCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCGAACCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACg                                                                                >  1:90821/1‑149 (MQ=255)                                                                tCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGtg                                                                              >  5:248520/1‑149 (MQ=255)                                                                    cATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGtgctgc                                                                          <  7:231323/149‑1 (MQ=255)                                                                    cATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGtgctgc                                                                          <  5:245050/149‑1 (MQ=255)                                                                      ttGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGtgctgc                                                                          >  6:193027/1‑147 (MQ=255)                                                                              cAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGa                                                                >  4:307793/1‑149 (MQ=255)                                                                              cAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGa                                                                <  8:223903/149‑1 (MQ=255)                                                                                aGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACCTGCTGCCAGTCGGCGACg                                                              <  8:18400/149‑1 (MQ=255)                                                                                  cTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGc                                                            <  1:287862/149‑1 (MQ=255)                                                                                  cTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGc                                                            <  8:262103/149‑1 (MQ=255)                                                                                      cgcCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGc                                                        <  3:17542/149‑1 (MQ=255)                                                                                         cAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCAcc                                                     <  6:111630/149‑1 (MQ=255)                                                                                         cAAAGGCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTGCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCAcc                                                     <  7:86344/149‑1 (MQ=255)                                                                                                gcgcATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTc                                                <  7:89842/147‑1 (MQ=255)                                                                                                   cATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGCCCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCt                                           >  7:70011/1‑149 (MQ=255)                                                                                                    aTAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCtg                                          >  1:237357/1‑149 (MQ=255)                                                                                                               aTGGTCG‑CCCGATACCCGGCTGCGCAGTCCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCa                               <  5:193027/149‑1 (MQ=255)                                                                                                                     g‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGc                         >  4:255646/1‑149 (MQ=255)                                                                                                                        ccGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCgggta                       >  1:137960/1‑146 (MQ=255)                                                                                                                               ccGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAg                <  6:248520/149‑1 (MQ=255)                                                                                                                                cGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGc               >  2:13365/1‑149 (MQ=255)                                                                                                                                cGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGc               <  2:237357/149‑1 (MQ=255)                                                                                                                                 ggCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCtt                                 <  3:270057/130‑1 (MQ=255)                                                                                                                                 ggCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCtt                                 >  4:270057/1‑130 (MQ=255)                                                                                                                                       gcAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCcag        >  4:230689/1‑149 (MQ=255)                                                                                                                                           tGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCcagcgca    <  3:307793/149‑1 (MQ=255)                                                                                                                                             ccccccGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCcagcgcagc  >  4:179457/1‑149 (MQ=255)                                                                                                                                              cccccGGCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCt                       >  7:24316/1‑127 (MQ=255)                                                                                                                                              cccccGGCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCt                       <  6:141576/127‑1 (MQ=255)                                                                                                                                              cccccGGCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCt                       >  5:141576/1‑127 (MQ=255)                                                                                                                                              cccccGGCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCt                       <  8:24316/127‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              TCGCGCAGTATCAGCTCGGCCAAACAGCGAAAAGGCACGCCCATGGCGCGGATGGCGCCGAGATCGGCATTGCACGCCAAGCTCGCGCCAAAGTCGCGCATAGCGCCAGCATGGTCG‑CCCGATACCCGGCTGCGCAGTGCCCCCCGCCATGCCAGAACGACAGCACGTTCCCTGGGCCTCAACGGGCGTTGTGACCAGTCTTCGAACACGTGCTGCCAGTCGGCGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC  >  NC_002947/5970158‑5970445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑