Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	668,264	A→C	7.9%	F87C (TTC→TGC) ‡	PP_0571 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	668,264	0	A	C	7.9%	196.5 / 7.1	63	F87C (TTC→TGC) ‡	PP_0571	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (28/30);  new base C (3/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAACCCGCTCAAT  >  NC_002947/668117‑668410                                                                                                                                                    |                                                                                                                                                   gAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGaa                                                                                                                                                   >  2:60130/1‑149 (MQ=255)    gcgtaaaGCCTGGGGCTGTTGTCCAGCCCTGTCGCGACTCACGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATgc                                                                                                                                                <  5:188247/145‑1 (MQ=255)      tGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGACGATGATCGAGGCGCGCATGAATgcgc                                                                                                                                              >  8:186885/1‑149 (MQ=255)        aaaGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGcgcg                                                                                                                                             >  2:142550/1‑148 (MQ=255)              tGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGcc                                                                                                                                      <  2:240501/149‑1 (MQ=255)               agggCTGCACAGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCCCAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGcc                                                                                                                                      >  3:100059/2‑148 (MQ=255)                gggCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGa                                                                                                                                    <  3:233332/149‑1 (MQ=255)                  gctgctGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGt                                                                                                                                  <  1:14965/149‑1 (MQ=255)                      ctgTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTAca                                                                                                                               <  8:208538/148‑1 (MQ=255)                           cAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAgcagca                                                                                                                         <  1:142550/149‑1 (MQ=255)                                   tCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGc                                                                                                                   <  7:62772/147‑1 (MQ=255)                                     gcgACGCAAGGCCGCTCCCAGAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgat                                                                                                               >  1:31957/1‑149 (MQ=255)                                      cgACGCAAGGCCGCTCCCAGAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgatg                                                                                                              <  2:31957/149‑1 (MQ=255)                                        aCGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgatgat                                                                                                            >  2:190794/1‑149 (MQ=255)                                         cGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgatgat                                                                                                            >  6:118041/1‑148 (MQ=255)                                          gCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATTCATGcgcgcct                                                                                                                                          <  7:235671/117‑4 (MQ=255)                                          gCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATTCATGcgcgcct                                                                                                                                          >  8:235671/1‑114 (MQ=255)                                            aaGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGGCATATGATACCGAGGATGCTCGAGGCGCGCATGAATCCCCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAgt                                                                                                        >  8:71791/1‑148 (MQ=255)                                            aaGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGcc                                                                                                                                      >  2:163163/1‑119 (MQ=255)                                            aaGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGcc                                                                                                                                      <  1:163163/119‑1 (MQ=255)                                            aaGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAgg                                                                                                        <  1:190794/149‑1 (MQ=255)                                                ccGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGc                                                                                                      >  2:203609/1‑147 (MQ=255)                                                  gCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAgg                                                                                                  <  5:118041/149‑1 (MQ=255)                                                   cTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGa                                                                                                 >  3:247599/1‑149 (MQ=255)                                                      ccACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTAcagc                                                                                                                             <  3:15654/118‑1 (MQ=255)                                                      ccACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTAcagc                                                                                                                             >  4:15654/1‑118 (MQ=255)                                                      ccACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgat                                                                                                               >  7:259964/1‑132 (MQ=255)                                                      ccACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCgat                                                                                                               <  8:259964/132‑1 (MQ=255)                                                      ccACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGc                                                                                              >  8:254289/1‑149 (MQ=255)                                                        acaAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCACGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCt                                                                                             <  1:119366/148‑1 (MQ=255)                                                         caAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTca                                                                                           <  1:203609/149‑1 (MQ=255)                                                          aaaGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTcag                                                                                          >  5:224249/1‑149 (MQ=255)                                                                 gcgcAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCg                                                                                     >  1:124048/1‑147 (MQ=255)                                                                 gcgcAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGaa                                                                                   <  1:60130/149‑1 (MQ=255)                                                                        tCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCa                                                                            <  6:224249/149‑1 (MQ=255)                                                                           gTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTc                                                                              <  4:304639/144‑1 (MQ=255)                                                                           gTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTc                                                                              <  4:244091/144‑1 (MQ=255)                                                                           gTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTc                                                                              >  3:244091/1‑144 (MQ=255)                                                                           gTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTc                                                                              >  3:304639/1‑144 (MQ=255)                                                                              gATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGccc                                                                       >  4:92425/1‑148 (MQ=255)                                                                                tCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAg                                                                    <  3:92425/149‑1 (MQ=255)                                                                                     cAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTgcg                                                               >  1:208252/1‑149 (MQ=255)                                                                                         tcatcaCCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGa                                                            >  3:136215/1‑148 (MQ=255)                                                                                           ctcaCCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCTGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAg                                                         <  8:229724/148‑1 (MQ=255)                                                                                              aCCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGcc                                                      <  4:19781/149‑1 (MQ=255)                                                                                              aCCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGc                                                       >  6:169074/1‑148 (MQ=255)                                                                                                  ataGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATc                                                   >  8:221579/3‑148 (MQ=255)                                                                                                    aGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGAt                                                >  7:85311/1‑149 (MQ=255)                                                                                                          aGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGtt                                          <  4:100059/149‑1 (MQ=255)                                                                                                            cACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGc                                        >  5:31275/1‑149 (MQ=255)                                                                                                               cagccagATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAg                                            <  5:66150/142‑1 (MQ=255)                                                                                                               cagccagATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAg                                            >  6:66150/1‑142 (MQ=255)                                                                                                                        gatTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTgg                            >  7:11268/1‑149 (MQ=255)                                                                                                                        gatTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTgg                            >  1:25243/1‑149 (MQ=255)                                                                                                                         atTCCGAGGATGATCGAGGCGCGCATGATTGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATTATCAGGTCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTgg                            <  7:71791/148‑1 (MQ=255)                                                                                                                          ttCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGcc                          <  2:25243/149‑1 (MQ=255)                                                                                                                             cGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCcagc                       <  7:254289/149‑1 (MQ=255)                                                                                                                                      tCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGaa               <  6:31275/148‑1 (MQ=255)                                                                                                                                          tccgcgcATTCATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCAtt                              <  3:40075/127‑1 (MQ=255)                                                                                                                                          tccgcgcATTCATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCAtt                              >  4:40075/3‑129 (MQ=255)                                                                                                                                           gcgcgcATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAcccc         >  7:255110/1‑149 (MQ=255)                                                                                                                                           ccgcgcATTCATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAAccc         >  1:61250/2‑149 (MQ=255)                                                                                                                                                  tGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAACCCGCTCAAt  <  4:247599/149‑1 (MQ=255)                                                                                                                                                  tGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAACCCGCTCAAt  <  5:169074/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAAGCTGAAAGCCTGGGGCTGCTGTGCAGCCCTGTCGCGACGCAAGGCCGCTCCCACAAAGACCGCGCAGATCAGTGGATCACCCAGCTCATCACCCACAGCCCCAGCACCAGCCAGATGATTCCGAGGATGATCGAGGCGCGCATGAATGCGCGGATTGCCGAGTACAGCAGCATCAGGCCGATGATCAGGGCCAGGATGCTCAGCAGCGAAGTGTCCATGCCCAAGGTGCGCGACAGGCCATCGATGAAGTTGCCACCGGCATTGGCCAGCAGGTTGAACAACCCGCTCAAT  >  NC_002947/668117‑668410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑