Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,994,277	T→C	6.7%	V335A (GTG→GCG)	PP_5251 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,994,277	0	T	C	6.7%	166.0 / 2.0	60	V335A (GTG→GCG)	PP_5251	transporter
Reads supporting (aligned to +/- strand):  ref base T (31/24);  new base C (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCCGTGACCGGCTGGTGTTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCCTGCTGGTGGCC  >  NC_002947/5994129‑5994425                                                                                                                                                     |                                                                                                                                                     aTCAGCCGTGACCGGCTGGTGTTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGt                                                                                                                                                      >  7:188679/1‑149 (MQ=255)      ccGTGACCGGCTGGTGTTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTg                                                                                                                                                 >  3:303870/1‑149 (MQ=255)               gCTGGTGTTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACtt                                                                                                                                        >  6:45346/1‑149 (MQ=255)                   gtgtTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCg                                                                                                                                      <  4:303870/147‑1 (MQ=255)                       tACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGt                                                                                                                                    >  4:220129/1‑145 (MQ=255)                       tACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGt                                                                                                                                    <  3:220129/145‑1 (MQ=255)                            gCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGAGCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCt                                                                                                                            <  7:199269/148‑1 (MQ=255)                                gCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTa                                                                                                                       <  8:188679/149‑1 (MQ=255)                                    gttgtATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACggg                                                                                                                   >  6:219135/1‑149 (MQ=255)                                          tGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCgg                                                                                                              <  6:68063/148‑1 (MQ=255)                                          tGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCgg                                                                                                              <  5:219135/148‑1 (MQ=255)                                            cccTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCt                                                                                                           >  8:202291/1‑149 (MQ=255)                                            cccTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCt                                                                                                           >  8:202293/1‑149 (MQ=255)                                             ccTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGTTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCgg                                                                                                              <  8:125792/145‑1 (MQ=255)                                             ccTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGTTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCgg                                                                                                              >  7:125792/1‑145 (MQ=255)                                              cTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGATGCCAGCCTGGGTACGGGCGCGGGCttt                                                                                                         >  4:191584/1‑149 (MQ=255)                                                   ccTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGTCGTGGATCGCGGTGCTGTCCAACCTGCAAGGGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCg                                                                                                     <  6:322562/148‑1 (MQ=255)                                                       ctgGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGATGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGta                                                                                                  <  3:191584/147‑1 (MQ=255)                                                         ggCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACa                                                                                              >  2:51614/1‑149 (MQ=255)                                                            ccTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTc                                                                                           >  4:135649/1‑149 (MQ=255)                                                              tGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGta                                                                                                  >  3:305877/1‑140 (MQ=255)                                                              tGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGta                                                                                                  <  4:305877/140‑1 (MQ=255)                                                                      gTGCGCAATTCCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTttctt                                                                                 <  3:19220/149‑1 (MQ=255)                                                                        gcgcAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCg                                                                               >  6:197121/1‑149 (MQ=255)                                                                        gcgcAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCg                                                                               >  3:319399/1‑149 (MQ=255)                                                                         cgcAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCgg                                                                              >  2:286189/1‑149 (MQ=255)                                                                              tttCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCATGGGTACGGGAGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCa                                                                         >  3:227418/1‑149 (MQ=255)                                                                                gctttGCCCTGCAGCCCGCGATGCTGCTCAGTGGCGGGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTTGGTACGGGCGCGGGCTTTGTCGGTATACATGCTGATTTTCTTCGGCGCCATg                                                                       <  8:222985/145‑1 (MQ=255)                                                                                      cgcTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCtgt                                                                 <  5:222120/149‑1 (MQ=255)                                                                                       gctgctGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCtgtg                                                                <  1:51614/149‑1 (MQ=255)                                                                                           ctgcCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTgtggtg                                                             <  7:202291/148‑1 (MQ=255)                                                                                           ctgcCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTgtggtg                                                             <  7:202293/148‑1 (MQ=255)                                                                                           ctgcCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGtggtgg                                                            >  5:148245/1‑149 (MQ=255)                                                                                              cccGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGc                                                           <  1:286189/147‑1 (MQ=255)                                                                                              cccGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGCGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCct                                                         >  4:310482/1‑149 (MQ=255)                                                                                              cccGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGCGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCct                                                         >  6:282789/1‑149 (MQ=255)                                                                                              cccGCGATGCTGCGCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGCACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCct                                                         <  4:189387/149‑1 (MQ=255)                                                                                                cgcgATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCctgc                                                       >  8:98504/1‑149 (MQ=255)                                                                                                     tgctgcTCAGTGGCGCGGCGTGGATCGCCGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTgg                                                  <  5:197121/149‑1 (MQ=255)                                                                                                      gctgctCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTggg                                                 >  4:3402/1‑149 (MQ=255)                                                                                                        tgctCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCtgt                                                    >  6:161477/1‑144 (MQ=255)                                                                                                        tgctCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCtgt                                                    <  5:161477/144‑1 (MQ=255)                                                                                                           tCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTgtggtg                                                             >  6:235050/1‑132 (MQ=255)                                                                                                           tCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTgtggtg                                                             <  5:235050/132‑1 (MQ=255)                                                                                                           tCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACg                                            >  6:244027/1‑149 (MQ=255)                                                                                                           tCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACg                                            >  6:52200/1‑149 (MQ=255)                                                                                                              gTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGCGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTg                                         <  3:310482/149‑1 (MQ=255)                                                                                                              gTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGCGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTg                                         <  5:282789/149‑1 (MQ=255)                                                                                                                 gcgcGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGgc                                       <  5:45346/148‑1 (MQ=255)                                                                                                                             tCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCAGGCTGGCCAGCCATGCAACg                          >  8:301036/1‑149 (MQ=255)                                                                                                                                cgGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGgc                                                                             <  2:252465/95‑1 (MQ=255)                                                                                                                                cgGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGgc                                                                             >  1:252465/1‑95 (MQ=255)                                                                                                                                cgGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCt                        >  6:123877/1‑148 (MQ=255)                                                                                                                                  ttGCTGTCCAACCTGCAAGTGGCTTCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTATCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCAc                     <  7:115799/148‑1 (MQ=255)                                                                                                                                       gTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCtt                  >  1:293742/1‑147 (MQ=255)                                                                                                                                        tCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAg               >  7:257420/1‑149 (MQ=255)                                                                                                                                             ccTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCctgc          >  8:7846/1‑149 (MQ=255)                                                                                                                                              cTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCctgct         >  2:267351/1‑149 (MQ=255)                                                                                                                                               tGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCctgctg        >  8:260103/1‑149 (MQ=255)                                                                                                                                                gCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCCTGCtgg       >  3:156695/1‑149 (MQ=255)                                                                                                                                                 cAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCctgctg        <  3:214769/147‑1 (MQ=255)                                                                                                                                                     tGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCCTGCTGGTGGcc  <  7:98504/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ATCAGCCGTGACCGGCTGGTGTTACTGGCCAGCCTGTTGTATGCCCTGTTCCTGCTGGCCCTGGCGTTGGTGCGCAATTTCTATGCGCTGCTGCCCGCGATGCTGCTCAGTGGCGCGGCGTGGATCGCGGTGCTGTCCAACCTGCAAGTGGCTGCACAGACTTCGGTGCCAGCCTGGGTACGGGCGCGGGCTTTGTCGGTATACATTCTGATTTTCTTCGGCGCCATGGCCTGTGGTGGCCTGCTGTGGGGCACGCTGGCCAGCCATGCAACGCTCACCTTCAGCCTGCTGGTGGCC  >  NC_002947/5994129‑5994425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑