Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 680,070 C→T 100% R319R (CGC→CGT)  PP_0582 → thiolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947680,0700CT100.0% 161.2 / ‑6.8 49R319R (CGC→CGT) PP_0582thiolase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/26);  minor base A (1/0);  total (23/26)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCAGCGAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGCAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTGGGCCTGGAGGCGCCGCTTGGCGCGATCGACC  >  NC_002947/679927‑680215
                                                                                                                                               |                                                                                                                                                 
ggCAGCGAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATgg                                                                                                                                              >  7:117717/1‑149 (MQ=255)
ggCAGCGAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGACGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATgg                                                                                                                                              <  1:60144/149‑1 (MQ=255)
  cAGCGAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGGGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGcc                                                                                                                                            >  1:4760/1‑149 (MQ=255)
      gAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGAc                                                                                                                                        >  2:147261/1‑149 (MQ=255)
       aGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACg                                                                                                                                       <  5:177112/149‑1 (MQ=255)
             gggCGCAGCAGCAGGGGTTGGAGGTGCTTGCGTTTCTGGTCGATGGCTATACCGCTGGGGTTGATTTTGTGTCGGGGCGCTAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGTCACGTAATGGCGTGACGGTGCAg                                                                                                                                 <  8:199647/149‑1 (MQ=255)
              ggCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAAt                                                                                                                                                >  1:146365/1‑133 (MQ=255)
              ggCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAAt                                                                                                                                                <  2:146365/133‑1 (MQ=255)
                cgcAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGAc                                                                                                                              >  3:271863/1‑149 (MQ=255)
                          ggggTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACttt                                                                                                                           >  1:66454/1‑142 (MQ=255)
                          ggggTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCGGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACttt                                                                                                                           <  2:66454/142‑1 (MQ=255)
                               tGCAGGTGCTTGCATATCTGGTCGATGCCGAAACCGCTGCGGTGGATTTTGTCACGGGGAGCGAAGGGATGTTGAAGGCGACGGTGTATGCCGTTCCGCCCTTGCTGGCACGAAATGGCCTGACGATGCAGGACTTTGACTACTAAGaa                                                                                                               >  2:114488/1‑149 (MQ=255)
                                gCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGTCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCGTGACGCTGCAGGACTTTGACTACTACGaaa                                                                                                              >  7:245348/1‑149 (MQ=255)
                                gCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGaaa                                                                                                              >  4:321829/1‑149 (MQ=255)
                                 cAGGTGCTTGCCTATATGGTCGATGGCGAAACCGCTGCGGTGGATTTTCTCACTGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATCCCGAGCCGCGCATTCTGCCACGTAATGGCCTGAGGCTGCAGGACTTTGACTACTACGaaa                                                                                                              >  8:201960/1‑148 (MQ=255)
                                   ggcgCTTGCCTATCTGGTCGAGGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATcc                                                                                                           <  2:21137/146‑1 (MQ=255)
                                    gTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCa                                                                                                          >  6:9429/1‑149 (MQ=255)
                                      gCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCACTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCAc                                                                                                         >  4:23052/1‑148 (MQ=255)
                                           ccTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGcc                                                                                                   <  8:119359/149‑1 (MQ=255)
                                            cTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGcc                                                                                                   >  3:48075/1‑148 (MQ=255)
                                            cTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGcc                                                                                                   <  4:48075/148‑1 (MQ=255)
                                                  ggTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCgg                                                                                            >  5:43023/1‑149 (MQ=255)
                                                   gTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGaaa                                                                                                              <  3:216964/130‑1 (MQ=255)
                                                   gTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGaaa                                                                                                              >  4:216964/1‑130 (MQ=255)
                                                    tCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGcc                                                                                          <  3:1678/149‑1 (MQ=255)
                                                      gATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCa                                                                                        <  1:322858/149‑1 (MQ=255)
                                                          gCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGt                                                                                     <  2:4760/148‑1 (MQ=255)
                                                          gCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTg                                                                                    <  8:117717/149‑1 (MQ=255)
                                                              aaCCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCtgt                                                                                <  1:147261/149‑1 (MQ=255)
                                                                ccGCTGCGGGGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGc                                                                              <  6:281538/149‑1 (MQ=255)
                                                                 cGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCa                                                                             <  2:169449/149‑1 (MQ=255)
                                                                        gTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCaa                                                                      <  5:9429/149‑1 (MQ=255)
                                                                          ggATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTg                                                                                               <  2:93291/122‑1 (MQ=255)
                                                                          ggATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTg                                                                                               >  1:93291/1‑122 (MQ=255)
                                                                               ttGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCACTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTg                                                                <  3:23052/148‑1 (MQ=255)
                                                                                    aCGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTggg                                                              >  5:239525/1‑145 (MQ=255)
                                                                                      ggggCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATg                                                        >  2:294363/1‑149 (MQ=255)
                                                                                       gggCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGc                                                       <  7:201960/149‑1 (MQ=255)
                                                                                                        gATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGaa                                       <  6:239525/148‑1 (MQ=255)
                                                                                                            gcgcCGGTTTATGGCGTGCAGCTCTTGCTGGCACGTAATGGCCTGACGGTGGAGGACTTTGACTAGTAGGAAATCCACGAAGGCTTTGCGGCCCAGGTGCTGTGCAGGCTCAAGGCCTGGGATGATGCCGATTACTGGCGTGAACGGtt                                  <  4:271863/149‑1 (MQ=255)
                                                                                                            gcgcCGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGtt                                  <  1:294363/149‑1 (MQ=255)
                                                                                                                cGGTGTATGCCGTGCCGCGCTTGCTGGCACGTAATGGCCTGACGCTGCATGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTGGGc                              <  6:43023/149‑1 (MQ=255)
                                                                                                                          cgtgccgCGCTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTgg                                >  2:253219/1‑137 (MQ=255)
                                                                                                                                gcgcTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTGGGCCTGGAGGCGCCGCTTg              >  4:250332/1‑149 (MQ=255)
                                                                                                                                  gcTTGCTGGCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGATGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCCTGAACGGTTGGGCCTGGAGGCGCCGGTTGgc            <  4:113821/149‑1 (MQ=255)
                                                                                                                                          gCACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTGGGCCTGGAGGCGCCGCTTGGCGCGATCGa    >  3:271816/1‑149 (MQ=255)
                                                                                                                                           cACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCg                                                     >  3:253588/1‑99 (MQ=255)
                                                                                                                                           cACGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCg                                                     <  4:253588/99‑1 (MQ=255)
                                                                                                                                            aCGTAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCAGTGAAAGGTTGGGCCTGGAGGCGCCGCTTGGCGCGATCGAcc  >  2:100370/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGCAGCGAGGCGTGGGCGCAGCAGCAGGGGTTGCAGGTGCTTGCCTATCTGGTCGATGGCGAAACCGCTGCGGTGGATTTTGTCACGGGGCGCGAAGGGTTGTTGATGGCGCCGGTGTATGCCGTGCCGCGCTTGCTGGCACGCAATGGCCTGACGCTGCAGGACTTTGACTACTACGAAATCCACGAAGCCTTTGCGGCCCAGGTGCTGTGCACGCTCAAGGCCTGGGAAGATGCCGATTACTGCCGTGAACGGTTGGGCCTGGAGGCGCCGCTTGGCGCGATCGACC  >  NC_002947/679927‑680215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑