Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,023,159 G→A 10.4% intergenic (+74/+70) PP_5273 → / ← PP_5274 oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,023,1590GA10.4% 182.4 / 8.3 68intergenic (+74/+70)PP_5273/PP_5274oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/33);  new base A (4/3);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

GGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGT  >  NC_002947/6023014‑6023302
                                                                                                                                                  |                                                                                                                                               
ggcggcAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGc                                                                                                                                              <  3:247757/149‑1 (MQ=255)
     cAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCt                                                                                                                                         >  1:136902/1‑149 (MQ=255)
      aaGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTc                                                                                                                                        <  8:1963/149‑1 (MQ=255)
      aaGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTc                                                                                                                                        >  5:227919/1‑149 (MQ=255)
       aGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCTcc                                                                                                                                       <  5:37072/149‑1 (MQ=255)
       aGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCTcc                                                                                                                                       <  7:80061/149‑1 (MQ=255)
            cTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAg                                                                                                                                  <  3:12596/149‑1 (MQ=255)
               cgcTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAgg                                                                                                                                 <  2:144718/147‑1 (MQ=255)
               cgcTCGACACTGAGGGCGCTTGGAATGCTGGGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAgg                                                                                                                                 <  6:227919/147‑1 (MQ=255)
                    gcgaCTGCTGGCGCTGGGAATGCTTTGGATTCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCTTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGTGCGTGCCCGCTCCCACAGGGTCGGt                                                                                                                           <  7:148035/145‑1 (MQ=255)
                       aCTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCaa                                                                                                                       <  4:218972/149‑1 (MQ=255)
                              gcgcTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCaa                                                                                                                >  8:104291/1‑149 (MQ=255)
                                     gAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg                                                                                                         <  8:227521/149‑1 (MQ=255)
                                       aTGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGa                                                                                                       <  2:274530/149‑1 (MQ=255)
                                        tGCTGTGGCATCGCCTCACAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGaa                                                                                                      <  7:298332/149‑1 (MQ=255)
                                           tgtgGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGaacaa                                                                                                   >  7:122232/1‑149 (MQ=255)
                                              ggCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAg                                                                                                <  7:285179/149‑1 (MQ=255)
                                               gCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg                                                                                               <  2:136902/149‑1 (MQ=255)
                                               gCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg                                                                                               <  8:18668/149‑1 (MQ=255)
                                               gCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg                                                                                               <  8:52327/149‑1 (MQ=255)
                                                 aTCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGcc                                                                                             <  7:104291/149‑1 (MQ=255)
                                                     ccTCAAAGAGCTGACTTGAAAGTCCCGCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTc                                                                                          >  1:172417/1‑149 (MQ=255)
                                                         aaaGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGt                                                                                                                           <  5:259055/111‑1 (MQ=255)
                                                         aaaGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGt                                                                                                                           >  6:259055/1‑111 (MQ=255)
                                                         aaaGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCGAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCg                                                                                     >  7:230165/1‑149 (MQ=255)
                                                         aaaGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAacaaca                                                                                                 <  3:238170/137‑1 (MQ=255)
                                                         aaaGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAacaaca                                                                                                 >  4:238170/1‑137 (MQ=255)
                                                                 gACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCcaca                                                                                                                                   >  5:177336/1‑95 (MQ=255)
                                                                 gACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCcaca                                                                                                                                   <  6:177336/95‑1 (MQ=255)
                                                                  aCTTGAAAGTCCC‑CATCCTTGTGGCAGCAAGTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCg                                                                                                                             >  1:27524/1‑100 (MQ=255)
                                                                  aCTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCg                                                                                                                             <  2:27524/100‑1 (MQ=255)
                                                                  aCTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                             <  4:174770/148‑1 (MQ=255)
                                                                    ttGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                           >  4:48687/1‑148 (MQ=255)
                                                                      gAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCa                                                                         <  1:108886/148‑1 (MQ=255)
                                                                             cc‑cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTggg                                                                                                         <  2:278203/109‑1 (MQ=255)
                                                                             cc‑cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTcc                                                                 <  3:48687/149‑1 (MQ=255)
                                                                             cc‑cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                  >  1:183771/1‑148 (MQ=255)
                                                                              c‑cATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCa                                                                <  2:172417/149‑1 (MQ=255)
                                                                                    cTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAgg                                                           <  2:183771/149‑1 (MQ=255)
                                                                                     ttGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAgg                                                           >  1:191990/1‑148 (MQ=255)
                                                                                       gtgGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAgg                                                           <  2:191990/146‑1 (MQ=255)
                                                                                         ggCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTc                                                      >  3:108909/1‑149 (MQ=255)
                                                                                           cagcaACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCg                                                    >  2:54090/1‑149 (MQ=255)
                                                                                               aaCTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCAGCTCCCACAGGGTAGGTGCAAGTTTAAAGTCTGGGGAAAAACAGGCCCTCCAGAGACCATCGTGTCAGTCCTTCAAGAAGGGTTTACGCCCt                                                >  7:156947/1‑149 (MQ=255)
                                                                                                aCTGTCTTGCTTAATATCTAAAAGGTGACGCGCCCCCTGTGGGAGCGGGCTTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCtt                                               <  7:220014/149‑1 (MQ=255)
                                                                                                 cTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTc                                              >  5:119272/1‑149 (MQ=255)
                                                                                                  tGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAACTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTAGCAGAAGGGTTTACGCCCTTc                                              >  8:109593/1‑148 (MQ=255)
                                                                                                      ttGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATcc                                         >  1:36749/1‑149 (MQ=255)
                                                                                                         cTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCgg                                       <  6:64494/148‑1 (MQ=255)
                                                                                                          ttAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                     >  7:174936/1‑149 (MQ=255)
                                                                                                            aaaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCt                                   >  8:40246/1‑149 (MQ=255)
                                                                                                                tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGATCAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                             >  1:106394/1‑119 (MQ=255)
                                                                                                                tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                             <  2:106394/119‑1 (MQ=255)
                                                                                                                 cTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                              >  5:331972/1‑149 (MQ=255)
                                                                                                                  tAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                              >  1:141410/1‑148 (MQ=255)
                                                                                                                    aaaGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTc                               <  3:162744/145‑1 (MQ=255)
                                                                                                                           aCGCGCCCCCTGGGGGAGCGGGCGTGGCCGCTCCCACAGGGGCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCGTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGc                     <  7:109593/148‑1 (MQ=255)
                                                                                                                                    cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATc             <  4:207173/147‑1 (MQ=255)
                                                                                                                                    cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATc             >  3:207173/1‑147 (MQ=255)
                                                                                                                                       tgGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCgcgc         >  8:6678/1‑148 (MQ=255)
                                                                                                                                        gggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGCCCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa       >  5:274642/1‑149 (MQ=255)
                                                                                                                                        gggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa       >  2:186555/1‑149 (MQ=255)
                                                                                                                                        gggAGCGGGAACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa       >  6:65060/1‑149 (MQ=255)
                                                                                                                                         ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc      <  7:40246/149‑1 (MQ=255)
                                                                                                                                         ggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                             >  8:173790/1‑94 (MQ=255)
                                                                                                                                         ggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                             <  7:173790/94‑1 (MQ=255)
                                                                                                                                          gAGCGGGCGTGCCCGCTCCCACAGGGGCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGc                     <  8:273873/133‑1 (MQ=255)
                                                                                                                                              gggCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGt  >  6:165833/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCC‑CATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGT  >  NC_002947/6023014‑6023302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑