Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 680,671 C→T 10.3% P64L (CCG→CTG)  PP_0583 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947680,6710CT10.3% 155.8 / 7.3 58P64L (CCG→CTG) PP_0583AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (4/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

ACCTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGC  >  NC_002947/680524‑680815
                                                                                                                                                   |                                                                                                                                                 
acCTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCg                                                                                                                                                  >  7:274619/1‑149 (MQ=255)
     cccAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGCACCACTCCCCCCCAGCACGACTGGGTGCAATTGGCCTACGCCAACAGCGGCGTACTCGGCGTGTACAAACGCGCCGGCAGTTACTTTGCCCCACCCCAGTg                                                                                                                                             >  5:175720/1‑149 (MQ=255)
      ccAGTCCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGTCATTTACTTTGCCCCACCGCAGTgg                                                                                                                                            <  8:119595/149‑1 (MQ=255)
       cAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTggg                                                                                                                                           <  2:232447/149‑1 (MQ=255)
            cccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtg                                                                                                                                      >  4:161458/1‑149 (MQ=255)
             ccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACTGCGGTggg                                                                                                                                           >  8:192762/1‑143 (MQ=255)
             ccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACTGCGGTggg                                                                                                                                           <  7:192762/143‑1 (MQ=255)
                 tACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                   <  8:56264/147‑1 (MQ=255)
                    gCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATccc                                                                                                                              >  1:150644/1‑149 (MQ=255)
                                aGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  8:274619/149‑1 (MQ=255)
                                aGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  2:150644/149‑1 (MQ=255)
                                 gCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAg                                                                                                                 <  6:314101/149‑1 (MQ=255)
                                  ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATcccc                                                                                                                             <  6:196772/136‑1 (MQ=255)
                                  ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATcccc                                                                                                                             >  5:196772/1‑136 (MQ=255)
                                                     aCCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  >  6:117297/1‑128 (MQ=255)
                                                     aCCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  5:117297/128‑1 (MQ=255)
                                                      ccACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATg                                                                                            <  4:317257/149‑1 (MQ=255)
                                                       cACTCGCCAGCAGCACGAGTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGt                                                                                                      <  7:39969/138‑1 (MQ=255)
                                                       cACTCGCCAACAGCACGAATGGGTCCAATTCTCCTACGCCATCAGCGGAGTACTAGGAGTGTAAACACGCGACGGCAGTTACATTCCCCCACCGCAGAGGGGGCTGTGGATACCCCCCGATCACGAGCATGAAGAgga                                                                                                      >  8:39969/1‑137 (MQ=255)
                                                           cGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGc                                                                                       >  2:93098/1‑149 (MQ=255)
                                                            gCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTACCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGcc                                                                                      >  1:161509/1‑149 (MQ=255)
                                                             ccaccaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCCATGCAAg                                                                                        >  6:185952/1‑146 (MQ=255)
                                                               accaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGAt                                                                                                                       <  2:240256/113‑1 (MQ=255)
                                                               accaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGAt                                                                                                                       >  1:240256/1‑113 (MQ=255)
                                                                        aCTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGc                                                                         <  6:248035/149‑1 (MQ=255)
                                                                              tGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTAc                                                                   <  5:185952/149‑1 (MQ=255)
                                                                               gCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgag                                                                                   <  6:288418/133‑1 (MQ=255)
                                                                               gCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgag                                                                                   >  5:288418/1‑133 (MQ=255)
                                                                                 aaTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgc                                                                                                                           >  1:307947/1‑91 (MQ=255)
                                                                                 aaTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgc                                                                                                                           <  2:307947/91‑1 (MQ=255)
                                                                                       ccTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGcgccgc                                                          >  4:107513/1‑149 (MQ=255)
                                                                                        cTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGGGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGcgccg                                                           <  6:175720/147‑1 (MQ=255)
                                                                                                  aGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGAtgcctgcc                                                >  4:1967/1‑148 (MQ=255)
                                                                                                      gcgTACTCGGCGTGTACACACGCGACGGCANTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATgg                                           >  1:29248/1‑149 (MQ=255)
                                                                                                      gcgTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATgg                                           <  3:161458/149‑1 (MQ=255)
                                                                                                             tcgCGTGTGTACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCAGGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCg                                     >  3:181765/3‑148 (MQ=255)
                                                                                                              ggCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGa                                   <  3:107513/149‑1 (MQ=255)
                                                                                                               gCGTGTACACACGCGACGGCAGTTACTTTACCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGaa                                  <  2:161509/149‑1 (MQ=255)
                                                                                                                cGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCTGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGaa                                  <  1:69415/149‑1 (MQ=255)
                                                                                                                    tACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATg                             >  4:236103/1‑149 (MQ=255)
                                                                                                                        cacGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         >  1:85450/1‑149 (MQ=255)
                                                                                                                         acGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCggg                        >  3:214394/1‑149 (MQ=255)
                                                                                                                               cGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTgg                  <  1:93098/149‑1 (MQ=255)
                                                                                                                                    gTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         >  6:104087/1‑137 (MQ=255)
                                                                                                                                    gTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         <  5:104087/137‑1 (MQ=255)
                                                                                                                                       aCTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAc           <  3:236103/148‑1 (MQ=255)
                                                                                                                                         tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTg                                                                >  5:319694/1‑93 (MQ=255)
                                                                                                                                         tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTg                                                                <  6:319694/93‑1 (MQ=255)
                                                                                                                                         tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAcgcc        >  3:230170/1‑149 (MQ=255)
                                                                                                                                            gCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  5:291742/1‑149 (MQ=255)
                                                                                                                                            cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAg               <  3:321978/138‑1 (MQ=255)
                                                                                                                                            cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAg               >  4:321978/2‑139 (MQ=255)
                                                                                                                                            cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  6:280241/2‑149 (MQ=255)
                                                                                                                                            cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  2:91044/2‑149 (MQ=255)
                                                                                                                                             ccccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTg    >  8:168557/1‑149 (MQ=255)
                                                                                                                                              cccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACTTGCCCCGCGATGCCTGCCCATGGCCGCCGGACCAATGCCGGGTGATGGAAGTGCCGCCGCTgc   >  3:10713/1‑148 (MQ=255)
                                                                                                                                              cccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCAAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTgg   >  4:24599/1‑149 (MQ=255)
                                                                                                                                               ccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGTGTGCTGGAAGTGACGCCGCTGgc  <  6:62576/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACCTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGC  >  NC_002947/680524‑680815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑