Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,042,339 A→T 9.1% E92D (GAA→GAT) ‡ PP_5295 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,042,3390AT 9.1% 152.7 / 4.4 54E92D (GAA→GAT) ‡PP_5295hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/28);  new base T (3/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCA  >  NC_002947/6042191‑6042483
                                                                                                                                                    |                                                                                                                                                
ccGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGaa                                                                                                                                                  <  4:293405/149‑1 (MQ=255)
     aGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                             <  4:38609/149‑1 (MQ=255)
           ggCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                       <  2:220729/149‑2 (MQ=255)
            gCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                      <  5:286398/149‑1 (MQ=255)
             cACCCGGGTCTGGGCGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgccgc                                                                                                                                     <  8:73235/149‑4 (MQ=255)
                  tGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                >  5:92050/1‑149 (MQ=255)
                         ggAGTTGCGTTCGGTCTCCGAGCGTTATGTGGATCCGCACCTGCGGCTGCCCGAAGGCGTGCGCGTCCTCGTAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  6:278201/149‑1 (MQ=255)
                         ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGa                                                                                                                           <  4:63937/147‑1 (MQ=255)
                         ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  4:163609/149‑1 (MQ=255)
                         ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  7:89133/149‑1 (MQ=255)
                          gAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAg                                                                                                                        <  3:237062/149‑1 (MQ=255)
                          gAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAg                                                                                                                        <  3:109588/149‑1 (MQ=255)
                               gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         >  7:103866/1‑143 (MQ=255)
                               gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  8:103866/143‑1 (MQ=255)
                               gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATg                                                                                                                   >  3:292841/1‑149 (MQ=255)
                                     ggTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCGTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCaaa                                                                                                              <  6:63791/148‑1 (MQ=255)
                                       tCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCg                                                                                                           >  2:273138/1‑149 (MQ=255)
                                         aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGc                                                                                                          <  2:186103/148‑1 (MQ=255)
                                          accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                         >  7:173348/1‑148 (MQ=255)
                                          accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTg                                                                                                        <  8:173348/149‑1 (MQ=255)
                                             accGTTATCTGGAGCCGCACCTGCGCCTGCCCGATGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGaaa                                                                                                     <  8:234347/149‑1 (MQ=255)
                                             accGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGaaa                                                                                                     <  5:52508/149‑1 (MQ=255)
                                                gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  <  5:145865/149‑1 (MQ=255)
                                                gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  >  1:130429/1‑149 (MQ=255)
                                                gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  >  6:221796/1‑149 (MQ=255)
                                                 ttATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg                                                                                                 <  3:216006/149‑1 (MQ=255)
                                                      tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGATAGTGGACCAt                                                                                            >  6:34995/1‑149 (MQ=255)
                                                      tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                            >  4:203367/1‑149 (MQ=255)
                                                        gAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGa                                                                                          <  3:119118/149‑1 (MQ=255)
                                                          gCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgc                                                                                        >  5:293053/1‑149 (MQ=255)
                                                                                      cgcgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGa                                                            <  6:92050/149‑1 (MQ=255)
                                                                                       gcgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAg                                                           >  3:110499/1‑149 (MQ=255)
                                                                                            cTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                      <  4:292841/149‑1 (MQ=255)
                                                                                            cTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                      >  6:302138/1‑149 (MQ=255)
                                                                                                 aGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctg                                                 <  4:110499/149‑1 (MQ=255)
                                                                                                 aGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctg                                                 <  3:203367/149‑1 (MQ=255)
                                                                                                           cGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATc                                         >  1:319475/1‑147 (MQ=255)
                                                                                                              gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                              <  4:8523/139‑1 (MQ=255)
                                                                                                              gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                              >  3:8523/1‑139 (MQ=255)
                                                                                                              gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAag                                      >  5:122939/1‑147 (MQ=255)
                                                                                                               aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAagcag                                   <  5:221796/149‑1 (MQ=255)
                                                                                                                    cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctgg                                                >  5:80823/1‑131 (MQ=255)
                                                                                                                    cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctgg                                                <  6:80823/131‑1 (MQ=255)
                                                                                                                          cAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCt                        >  8:298124/1‑149 (MQ=255)
                                                                                                                                cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCaa                                       >  3:310802/1‑128 (MQ=255)
                                                                                                                                cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCaa                                       <  4:310802/128‑1 (MQ=255)
                                                                                                                                 tGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCg                 >  2:42699/1‑149 (MQ=255)
                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTg              <  7:298124/148‑1 (MQ=255)
                                                                                                                                      cgcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGa            <  5:302138/149‑1 (MQ=255)
                                                                                                                                       gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGaa           <  2:319475/149‑1 (MQ=255)
                                                                                                                                         cgCAAGGTGGATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGCCCATGAGCGCGCCGCGGAACTGGTTCACGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCGGAACCCGCTGGAAGt         >  6:210651/2‑149 (MQ=255)
                                                                                                                                         cgCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGt         >  8:301343/2‑149 (MQ=255)
                                                                                                                                          gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGAAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTCGTTGCCGCCGCCGAGGAAGTGGCTGCACTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTg        >  1:132938/1‑149 (MQ=255)
                                                                                                                                           cAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTGc       >  1:249567/1‑149 (MQ=255)
                                                                                                                                                tGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTGCTGTCa  >  1:277303/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCA  >  NC_002947/6042191‑6042483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑