Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,042,341 G→C 9.3% C93S (TGC→TCC)  PP_5295 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,042,3410GC 9.3% 160.8 / 7.4 54C93S (TGC→TCC) PP_5295hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base C (3/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

AGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCA  >  NC_002947/6042196‑6042483
                                                                                                                                                 |                                                                                                                                              
aGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                             <  4:38609/149‑1 (MQ=255)
      ggCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                       <  2:220729/149‑2 (MQ=255)
       gCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                      <  5:286398/149‑1 (MQ=255)
        cACCCGGGTCTGGGCGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgccgc                                                                                                                                     <  8:73235/149‑4 (MQ=255)
             tGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                >  5:92050/1‑149 (MQ=255)
                    ggAGTTGCGTTCGGTCTCCGAGCGTTATGTGGATCCGCACCTGCGGCTGCCCGAAGGCGTGCGCGTCCTCGTAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  6:278201/149‑1 (MQ=255)
                    ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGa                                                                                                                           <  4:63937/147‑1 (MQ=255)
                    ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  7:89133/149‑1 (MQ=255)
                    ggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  4:163609/149‑1 (MQ=255)
                     gAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAg                                                                                                                        <  3:109588/149‑1 (MQ=255)
                     gAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAg                                                                                                                        <  3:237062/149‑1 (MQ=255)
                          gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         <  8:103866/143‑1 (MQ=255)
                          gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                         >  7:103866/1‑143 (MQ=255)
                          gCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATg                                                                                                                   >  3:292841/1‑149 (MQ=255)
                                ggTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCGTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCaaa                                                                                                              <  6:63791/148‑1 (MQ=255)
                                  tCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCg                                                                                                           >  2:273138/1‑149 (MQ=255)
                                    aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGc                                                                                                          <  2:186103/148‑1 (MQ=255)
                                     accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                         >  7:173348/1‑148 (MQ=255)
                                     accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTg                                                                                                        <  8:173348/149‑1 (MQ=255)
                                        accGTTATCTGGAGCCGCACCTGCGCCTGCCCGATGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGaaa                                                                                                     <  8:234347/149‑1 (MQ=255)
                                        accGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGaaa                                                                                                     <  5:52508/149‑1 (MQ=255)
                                           gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  >  6:221796/1‑149 (MQ=255)
                                           gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  <  5:145865/149‑1 (MQ=255)
                                           gTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                  >  1:130429/1‑149 (MQ=255)
                                            ttATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTgg                                                                                                 <  3:216006/149‑1 (MQ=255)
                                                 tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGATAGTGGACCAt                                                                                            >  6:34995/1‑149 (MQ=255)
                                                 tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                            >  4:203367/1‑149 (MQ=255)
                                                   gAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGa                                                                                          <  3:119118/149‑1 (MQ=255)
                                                     gCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgc                                                                                        >  5:293053/1‑149 (MQ=255)
                                                                                 cgcgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGa                                                            <  6:92050/149‑1 (MQ=255)
                                                                                  gcgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAg                                                           >  3:110499/1‑149 (MQ=255)
                                                                                       cTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                      >  6:302138/1‑149 (MQ=255)
                                                                                       cTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                      <  4:292841/149‑1 (MQ=255)
                                                                                            aGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctg                                                 <  4:110499/149‑1 (MQ=255)
                                                                                            aGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctg                                                 <  3:203367/149‑1 (MQ=255)
                                                                                                      cGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATc                                         >  1:319475/1‑147 (MQ=255)
                                                                                                         gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                              <  4:8523/139‑1 (MQ=255)
                                                                                                         gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                              >  3:8523/1‑139 (MQ=255)
                                                                                                         gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAag                                      >  5:122939/1‑147 (MQ=255)
                                                                                                          aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAagcag                                   <  5:221796/149‑1 (MQ=255)
                                                                                                               cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctgg                                                >  5:80823/1‑131 (MQ=255)
                                                                                                               cTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctgg                                                <  6:80823/131‑1 (MQ=255)
                                                                                                                     cAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCt                        >  8:298124/1‑149 (MQ=255)
                                                                                                                           cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCaa                                       <  4:310802/128‑1 (MQ=255)
                                                                                                                           cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCaa                                       >  3:310802/1‑128 (MQ=255)
                                                                                                                            tGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCg                 >  2:42699/1‑149 (MQ=255)
                                                                                                                                aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTg              <  7:298124/148‑1 (MQ=255)
                                                                                                                                 cgcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGa            <  5:302138/149‑1 (MQ=255)
                                                                                                                                  gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGaa           <  2:319475/149‑1 (MQ=255)
                                                                                                                                    cgCAAGGTGGATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGCCCATGAGCGCGCCGCGGAACTGGTTCACGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCGGAACCCGCTGGAAGt         >  6:210651/2‑149 (MQ=255)
                                                                                                                                    cgCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGt         >  8:301343/2‑149 (MQ=255)
                                                                                                                                     gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGAAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTCGTTGCCGCCGCCGAGGAAGTGGCTGCACTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTg        >  1:132938/1‑149 (MQ=255)
                                                                                                                                      cAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTGc       >  1:249567/1‑149 (MQ=255)
                                                                                                                                           tGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGCCCCCCCTGAACCCGCTGGAAGTGCTGTCa  >  1:277303/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCA  >  NC_002947/6042196‑6042483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑