Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,049,795 G→A 7.2% S219S (TCG→TCA)  spoT → bifunctional (p)ppGpp synthase/hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,049,7950GA 7.2% 156.0 / 2.0 55S219S (TCG→TCA) spoTbifunctional (p)ppGpp synthase/hydrolase
Reads supporting (aligned to +/- strand):  ref base G (28/23);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GCCTGGGGATGCACACCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCATCGTCGAC  >  NC_002947/6049653‑6049942
                                                                                                                                              |                                                                                                                                                    
gCCTGGGGATGCACACCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGc                                                                                                                                                 >  4:175886/1‑148 (MQ=255)
     gggATGCACACCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTTATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGGGCACTCGCTGGCCAACTg                                                                                                                                           >  1:295076/1‑149 (MQ=255)
            acacCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGgcc                                                                                                                                    >  2:89600/1‑149 (MQ=255)
              acCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGgccgc                                                                                                                                  >  3:201444/1‑149 (MQ=255)
                 gTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCGGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGa                                                                                                                               <  7:174774/149‑1 (MQ=255)
                           agTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAg                                                                                                                     >  3:278142/1‑149 (MQ=255)
                             ttCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAggg                                                                                                                   >  5:27754/1‑149 (MQ=255)
                                gAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCACTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCg                                                                                                                 >  8:13162/1‑148 (MQ=255)
                                   gACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCACTGGCCAACTGCCTGGCCGCCGACGGCATCGAgg                                                                                                                    <  4:307129/142‑1 (MQ=255)
                                   gACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCACTGGCCAACTGCCTGGCCGCCGACGGCATCGAgg                                                                                                                    >  3:307129/1‑142 (MQ=255)
                                     ccTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGt                                                                                                             >  5:93217/1‑147 (MQ=255)
                                      cTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAg                                                                                                          >  4:49878/1‑149 (MQ=255)
                                      cTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAg                                                                                                          >  7:307609/1‑149 (MQ=255)
                                           cTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTc                                                                                                     >  5:292749/1‑149 (MQ=255)
                                            ttCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCg                                                                                                    >  1:217258/1‑149 (MQ=255)
                                              cAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCgg                                                                                                   <  3:175886/148‑1 (MQ=255)
                                               aaGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCgg                                                                                                   >  3:153763/1‑147 (MQ=255)
                                                 ggCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAg                                                                                                          <  7:36347/138‑1 (MQ=255)
                                                 ggCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAg                                                                                                          >  8:36347/1‑138 (MQ=255)
                                                     aTGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAaca                                                                                           >  8:290224/1‑149 (MQ=255)
                                                          cccGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACctct                                                                                      <  6:292749/149‑1 (MQ=255)
                                                             gATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATg                                                                                   <  1:89600/149‑1 (MQ=255)
                                                                 cgcTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCAt                                                                               <  7:290224/149‑1 (MQ=255)
                                                                  gcTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACctc                                                                                       <  3:240925/140‑1 (MQ=255)
                                                                  gcTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACctc                                                                                       >  4:240925/1‑140 (MQ=255)
                                                                    tcgtcgCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAacac                                                                                          >  2:284305/1‑135 (MQ=255)
                                                                    tcgtcgCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAacac                                                                                          <  1:284305/135‑1 (MQ=255)
                                                                          cTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACaaga‑a                                                                     <  6:93217/149‑1 (MQ=255)
                                                                            gATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACaag                                                                        >  7:51879/1‑145 (MQ=255)
                                                                             aTTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGAt                                                                  >  1:220807/1‑149 (MQ=255)
                                                                                  tCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCACTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGcgcg                                                             <  7:13162/149‑1 (MQ=255)
                                                                                               agCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGc                                                <  4:278142/149‑1 (MQ=255)
                                                                                               agCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGc                                                >  7:263838/1‑149 (MQ=255)
                                                                                                  gcgcgcgGCAACCGCAAAGAGATCGTCGCCAAGATCGGGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCtt                                             <  2:295076/149‑1 (MQ=255)
                                                                                                    gcgcgGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGATAGATGCGCGGCAAGCGCCGTGCCTTc                                            <  1:3557/149‑1 (MQ=255)
                                                                                                     cgcgGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCTAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCaa                                          <  2:217258/149‑1 (MQ=255)
                                                                                                      gcgGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAAc                                         <  3:49878/149‑1 (MQ=255)
                                                                                                          cAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACgaga                                     <  8:263838/149‑1 (MQ=255)
                                                                                                               gCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATg                                >  3:49682/1‑149 (MQ=255)
                                                                                                                cAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATg                                >  7:89772/1‑148 (MQ=255)
                                                                                                                  aaGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGAc                             >  2:105921/1‑149 (MQ=255)
                                                                                                                  aaGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGAGGCCAGCGGCGGGCCTTCCACGAGTACATGGAc                             >  8:85580/1‑149 (MQ=255)
                                                                                                                        tcgtcgCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTAt                       <  6:27754/149‑1 (MQ=255)
                                                                                                                         cgtcgCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATg                      >  7:289577/1‑149 (MQ=255)
                                                                                                                             gCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACct                                                                                        >  6:300346/1‑80 (MQ=255)
                                                                                                                             gCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACct                                                                                        <  5:300346/80‑1 (MQ=255)
                                                                                                                              ccAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTc                 <  8:51879/149‑1 (MQ=255)
                                                                                                                               cAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTcc                <  8:289577/149‑1 (MQ=255)
                                                                                                                               cAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTcc                >  1:32820/1‑149 (MQ=255)
                                                                                                                                aaGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCg               <  4:201444/149‑1 (MQ=255)
                                                                                                                                   aTCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGcat            <  4:153763/149‑1 (MQ=255)
                                                                                                                                     cGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGcatca          >  6:98815/1‑149 (MQ=255)
                                                                                                                                      gAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGcatcat         >  1:265015/1‑149 (MQ=255)
                                                                                                                                        gCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCAtcg       <  8:89772/149‑1 (MQ=255)
                                                                                                                                          aCTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCAtcgtc     <  5:98815/149‑1 (MQ=255)
                                                                                                                                              gCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCATCGTCGAc  >  8:277787/1‑148 (MQ=255)
                                                                                                                                              gCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCATCGTCGAc  <  8:307609/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCCTGGGGATGCACACCGTGCGCGTAGAGTTCGAAGACCTTGGCTTCAAGGCCATGCACCCGATGCGCTCGTCGCTGATTCATCGTGCAGTGAAGAGCGCGCGCGGCAACCGCAAAGAGATCGTCGCCAAGATCGAGCACTCGCTGGCCAACTGCCTGGCCGCCGACGGCATCGAGGGCGAAGTCAGCGGTCGGCAGAAACACCTCTATGGCATCTACAAGA‑AGATGCGCGGCAAGCGCCGTGCCTTCAACGAGATCATGGACGTGTATGCCTTCCGCATCATCGTCGAC  >  NC_002947/6049653‑6049942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑