Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	680,674	A→G	10.5%	Q65R (CAG→CGG)	PP_0583 →	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	680,674	0	A	G	10.5%	149.7 / 7.1	57	Q65R (CAG→CGG)	PP_0583	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base G (4/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGC  >  NC_002947/680529‑680815                                                                                                                                                  |                                                                                                                                               cccAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGCACCACTCCCCCCCAGCACGACTGGGTGCAATTGGCCTACGCCAACAGCGGCGTACTCGGCGTGTACAAACGCGCCGGCAGTTACTTTGCCCCACCCCAGTg                                                                                                                                             >  5:175720/1‑149 (MQ=255)  ccAGTCCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGTCATTTACTTTGCCCCACCGCAGTgg                                                                                                                                            <  8:119595/149‑1 (MQ=255)   cAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTggg                                                                                                                                           <  2:232447/149‑1 (MQ=255)        cccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtg                                                                                                                                      >  4:161458/1‑149 (MQ=255)         ccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACTGCGGTggg                                                                                                                                           >  8:192762/1‑143 (MQ=255)         ccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACTGCGGTggg                                                                                                                                           <  7:192762/143‑1 (MQ=255)             tACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                   <  8:56264/147‑1 (MQ=255)                gCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATccc                                                                                                                              >  1:150644/1‑149 (MQ=255)                            aGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  8:274619/149‑1 (MQ=255)                            aGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  2:150644/149‑1 (MQ=255)                             gCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAg                                                                                                                 <  6:314101/149‑1 (MQ=255)                              ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATcccc                                                                                                                             <  6:196772/136‑1 (MQ=255)                              ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATcccc                                                                                                                             >  5:196772/1‑136 (MQ=255)                                                 aCCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  >  6:117297/1‑128 (MQ=255)                                                 aCCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGa                                                                                                                  <  5:117297/128‑1 (MQ=255)                                                  ccACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATg                                                                                            <  4:317257/149‑1 (MQ=255)                                                   cACTCGCCAGCAGCACGAGTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGt                                                                                                      <  7:39969/138‑1 (MQ=255)                                                   cACTCGCCAACAGCACGAATGGGTCCAATTCTCCTACGCCATCAGCGGAGTACTAGGAGTGTAAACACGCGACGGCAGTTACATTCCCCCACCGCAGAGGGGGCTGTGGATACCCCCCGATCACGAGCATGAAGAgga                                                                                                      >  8:39969/1‑137 (MQ=255)                                                       cGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGc                                                                                       >  2:93098/1‑149 (MQ=255)                                                        gCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTACCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGcc                                                                                      >  1:161509/1‑149 (MQ=255)                                                         ccaccaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCCATGCAAg                                                                                        >  6:185952/1‑146 (MQ=255)                                                           accaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGAt                                                                                                                       >  1:240256/1‑113 (MQ=255)                                                           accaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGAt                                                                                                                       <  2:240256/113‑1 (MQ=255)                                                                    aCTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGc                                                                         <  6:248035/149‑1 (MQ=255)                                                                          tGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTAc                                                                   <  5:185952/149‑1 (MQ=255)                                                                           gCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgag                                                                                   <  6:288418/133‑1 (MQ=255)                                                                           gCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgag                                                                                   >  5:288418/1‑133 (MQ=255)                                                                             aaTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgc                                                                                                                           >  1:307947/1‑91 (MQ=255)                                                                             aaTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgc                                                                                                                           <  2:307947/91‑1 (MQ=255)                                                                                   ccTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGcgccgc                                                          >  4:107513/1‑149 (MQ=255)                                                                                    cTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGGGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGcgccg                                                           <  6:175720/147‑1 (MQ=255)                                                                                              aGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGAtgcctgcc                                                >  4:1967/1‑148 (MQ=255)                                                                                                  gcgTACTCGGCGTGTACACACGCGACGGCANTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATgg                                           >  1:29248/1‑149 (MQ=255)                                                                                                  gcgTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATgg                                           <  3:161458/149‑1 (MQ=255)                                                                                                         tcgCGTGTGTACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCAGGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCg                                     >  3:181765/3‑148 (MQ=255)                                                                                                          ggCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGa                                   <  3:107513/149‑1 (MQ=255)                                                                                                           gCGTGTACACACGCGACGGCAGTTACTTTACCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGaa                                  <  2:161509/149‑1 (MQ=255)                                                                                                            cGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCTGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGaa                                  <  1:69415/149‑1 (MQ=255)                                                                                                                tACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATg                             >  4:236103/1‑149 (MQ=255)                                                                                                                    cacGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         >  1:85450/1‑149 (MQ=255)                                                                                                                     acGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCggg                        >  3:214394/1‑149 (MQ=255)                                                                                                                           cGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTgg                  <  1:93098/149‑1 (MQ=255)                                                                                                                                gTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         <  5:104087/137‑1 (MQ=255)                                                                                                                                gTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                         >  6:104087/1‑137 (MQ=255)                                                                                                                                   aCTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAc           <  3:236103/148‑1 (MQ=255)                                                                                                                                     tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTg                                                                <  6:319694/93‑1 (MQ=255)                                                                                                                                     tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTg                                                                >  5:319694/1‑93 (MQ=255)                                                                                                                                     tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAcgcc        >  3:230170/1‑149 (MQ=255)                                                                                                                                        gCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  5:291742/1‑149 (MQ=255)                                                                                                                                        cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAg               >  4:321978/2‑139 (MQ=255)                                                                                                                                        cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAg               <  3:321978/138‑1 (MQ=255)                                                                                                                                        cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  6:280241/2‑149 (MQ=255)                                                                                                                                        cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt     >  2:91044/2‑149 (MQ=255)                                                                                                                                         ccccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTg    >  8:168557/1‑149 (MQ=255)                                                                                                                                          cccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACTTGCCCCGCGATGCCTGCCCATGGCCGCCGGACCAATGCCGGGTGATGGAAGTGCCGCCGCTgc   >  3:10713/1‑148 (MQ=255)                                                                                                                                          cccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCAAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTgg   >  4:24599/1‑149 (MQ=255)                                                                                                                                           ccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGTGTGCTGGAAGTGACGCCGCTGgc  <  6:62576/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               CCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGC‑GCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGC  >  NC_002947/680529‑680815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑