Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,177,327 A→G 7.8% I53T (ATC→ACC)  atpG ← ATP synthase subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,177,3270AG 7.8% 144.7 / 2.6 51I53T (ATC→ACC) atpGATP synthase subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (22/25);  new base G (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

TCCTTGACCAGGGCCTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTT  >  NC_002947/6177180‑6177472
                                                                                                                                                    |                                                                                                                                                 
tCCTTGACCAGGGCCTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGAt                                                                                                                                                  <  3:231830/149‑1 (MQ=255)
       ccAGGGCCTTGAACAGGTTGGTGTTCAAGCCACCGCACGGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTgg                                                                                                                                           <  8:164561/149‑1 (MQ=255)
       ccAGGGCCTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTgg                                                                                                                                           >  2:19066/1‑149 (MQ=255)
            ggcTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGGTGATCGGTCATCTGGccaac                                                                                                                                      <  2:111464/147‑5 (MQ=255)
             ccTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCt                                                                                                                                             >  8:155656/1‑141 (MQ=255)
             ccTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCt                                                                                                                                             <  7:155656/141‑1 (MQ=255)
                   aCAGGTTGGAGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTcc                                                                                                                               >  3:33502/1‑149 (MQ=255)
                      ggTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCcgcg                                                                                                                            <  6:106442/149‑1 (MQ=255)
                            tgtTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGa                                                                                                                      >  5:180329/1‑149 (MQ=255)
                                cAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACgg                                                                                                                   <  2:23016/148‑1 (MQ=255)
                                 aaGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGc                                                                                                                  <  3:51089/148‑1 (MQ=255)
                                 aaGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGc                                                                                                                  >  6:50900/1‑148 (MQ=255)
                                   gCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTag                                                                                                               <  6:180329/149‑1 (MQ=255)
                                        cGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTagcagc                                                                                                           >  6:204010/1‑148 (MQ=255)
                                          cacaGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCAt                                                                                                        <  5:204010/149‑1 (MQ=255)
                                                 cACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCAtt                                                                                                 >  6:187466/1‑149 (MQ=255)
                                                    ggTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTtgt                                                                                              <  2:299404/149‑1 (MQ=255)
                                                       cACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGcc                                                                                           <  4:33502/149‑1 (MQ=255)
                                                         cTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCtt                                                                                         <  3:317955/149‑1 (MQ=255)
                                                          tGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGGACGGGTGGCGGTATTCCGGGTTGGCGTTTGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTtgt                                                                                              <  5:238427/143‑1 (MQ=255)
                                                          tGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGGACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTtgt                                                                                              >  6:238427/1‑143 (MQ=255)
                                                          tGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTg                                                                                        >  5:87687/1‑149 (MQ=255)
                                                            cTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTgc                                                                                       >  7:41102/1‑148 (MQ=255)
                                                            cTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTgc                                                                                       <  8:41102/148‑1 (MQ=255)
                                                               accacGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCAt                                                                                   <  7:183483/149‑1 (MQ=255)
                                                                 cacGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCt                                                                                 >  8:4019/1‑149 (MQ=255)
                                                                    gATATAACCAGCGCGCTTTACAGGGCGGCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTg                                                                               >  1:4407/1‑149 (MQ=255)
                                                                             aGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCa                                                                     >  5:270802/1‑149 (MQ=255)
                                                                                       aCAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCtt                                                                                <  4:988/128‑1 (MQ=255)
                                                                                       aCAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCtt                                                                                >  3:988/1‑128 (MQ=255)
                                                                                            gc‑gcTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGgcgc                                                      <  4:169506/149‑1 (MQ=255)
                                                                                               gcTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTg                                                    >  2:164956/1‑149 (MQ=255)
                                                                                               gcTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTg                                                    <  7:4019/149‑1 (MQ=255)
                                                                                                   gATCATGAACGGGTGGCGGTAATCCGGCATGCCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATATGTGCCTTGCGCATCTTGCTCACCGCCACTTTCTCCATGGCCCTGGTaa                                                >  2:319372/1‑149 (MQ=255)
                                                                                                     tCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAAtt                                              <  1:19066/149‑1 (MQ=255)
                                                                                                           aCGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGc                                        <  6:87687/149‑1 (MQ=255)
                                                                                                                        ttCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGc                           >  6:125056/1‑149 (MQ=255)
                                                                                                                           cGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTc                         <  2:4407/148‑1 (MQ=255)
                                                                                                                              gttggcgttggcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCaa                     >  7:120782/1‑149 (MQ=255)
                                                                                                                                   cgttggcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTa                <  2:172099/149‑1 (MQ=255)
                                                                                                                                   cgttggcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTa                <  5:50900/149‑1 (MQ=255)
                                                                                                                                    gttggcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTAc               >  3:186069/1‑149 (MQ=255)
                                                                                                                                     ttggcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACt              <  6:270802/149‑1 (MQ=255)
                                                                                                                                     tccgcCAGGTGATCGGTCATCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACt              >  7:267121/4‑149 (MQ=255)
                                                                                                                                        gcCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGc            >  5:185309/1‑148 (MQ=255)
                                                                                                                                          cAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAAtttttt                                          >  3:263248/1‑116 (MQ=255)
                                                                                                                                          cAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAAtttttt                                          <  4:263248/116‑1 (MQ=255)
                                                                                                                                          cAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGaa         >  8:282486/1‑149 (MQ=255)
                                                                                                                                            ggtGATCGGTCATCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCttt                                 >  4:116150/3‑123 (MQ=255)
                                                                                                                                            ggtGATCGGTCATCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCttt                                 <  3:116150/121‑1 (MQ=255)
                                                                                                                                                 ccGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCttt  <  4:186069/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCCTTGACCAGGGCCTTGAACAGGTTGGTGTTCAAGCCACCGCACAGACCACGGTCACTGCTCACCACGATATAACCAGCGCGCTTTACAGGGC‑GCTCGATCATGAACGGGTGGCGGTATTCCGGGTTGGCGTTGGCCAGATGACCGATCACCTGGCGGATACGTTCCGCGTAAGGACGGCTAGCAGCCATGCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTT  >  NC_002947/6177180‑6177472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑