Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 720,921 G→C 8.5% T92S (ACC→AGC) ‡ PP_0614 ← bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947720,9210GC 8.5% 148.3 / 3.9 47T92S (ACC→AGC) ‡PP_0614bifunctional N‑carbamoyl‑beta‑alanine amidohydrolase/allantoine amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAGGCG  >  NC_002947/720778‑721069
                                                                                                                                               |                                                                                                                                                    
cGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTc                                                                                                                                                    <  7:115790/146‑1 (MQ=255)
      tCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGggc                                                                                                                                           <  1:87467/149‑1 (MQ=255)
             caccacTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGCCCATCATGACCggcagcc                                                                                                                                    <  5:218345/149‑3 (MQ=25)
               ccacTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCggcagggca                                                                                                                                  <  2:100777/149‑1 (MQ=255)
                       aGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCTGCCCATCATGACCggcagcc                                                                                                                                    <  2:43728/139‑3 (MQ=25)
                       aGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTgcg                                                                                                                          >  8:190206/1‑149 (MQ=255)
                       aGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCAGCCCATCATGACCggcagcc                                                                                                                                    >  1:43728/1‑137 (MQ=25)
                           gggCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCt                                                                                                                      <  6:312187/149‑1 (MQ=255)
                                      gtCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTc                                                                                                                                                    >  8:238822/1‑108 (MQ=255)
                                      gtCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTc                                                                                                                                                    <  7:238822/108‑1 (MQ=255)
                                      gtCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGGGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggc                                                                                                           <  6:133438/149‑1 (MQ=255)
                                       tCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGcagggcaggg                                                                                                                               >  3:99143/1‑128 (MQ=255)
                                       tCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGcagggcaggg                                                                                                                               <  4:99143/128‑1 (MQ=255)
                                       tCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggc                                                                                                           >  2:36747/1‑148 (MQ=255)
                                         aGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCATCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGaa                                                                                                        <  5:298486/149‑1 (MQ=255)
                                          ggTGCATGTAGTTGTGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGGCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAg                                                                                                       <  4:208270/149‑1 (MQ=255)
                                           gTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggcg                                                                                                          >  7:276174/1‑145 (MQ=255)
                                            tGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGat                                                                                                     <  7:190206/149‑1 (MQ=255)
                                                ggTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTg                                                                                                 <  2:151426/149‑1 (MQ=255)
                                                         gCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCg                                                                                        >  4:286198/1‑149 (MQ=255)
                                                           gTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTc                                                                                      >  7:275126/1‑149 (MQ=255)
                                                               gcATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACg                                                                                  >  7:285601/1‑149 (MQ=255)
                                                                 aTCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCt                                                                                >  1:208446/1‑149 (MQ=255)
                                                                     cTTCGAGGCCGGCCATGACGCCGTAGCAGCCACCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGAcc                                                                            >  6:18204/1‑149 (MQ=255)
                                                                      ttCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggc                                                                                                           >  1:136756/1‑117 (MQ=255)
                                                                      ttCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCggcgggc                                                                                                           <  2:136756/117‑1 (MQ=255)
                                                                        cGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGt                                                                          <  5:2360/148‑1 (MQ=255)
                                                                            gccggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGc                                                                     <  4:43508/149‑1 (MQ=255)
                                                                            gccggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGc                                                                     >  5:94323/1‑149 (MQ=255)
                                                                               ggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCgg                                                                  <  4:93209/149‑1 (MQ=255)
                                                                               ggccATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCgg                                                                  <  2:208446/149‑1 (MQ=255)
                                                                                    tGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGt                                                                                       >  8:171073/1‑123 (MQ=255)
                                                                                    tGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGt                                                                                       <  7:171073/123‑1 (MQ=255)
                                                                                        gCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGAc                                                                                   >  5:164617/1‑123 (MQ=255)
                                                                                        gCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGAc                                                                                   <  6:164617/123‑1 (MQ=255)
                                                                                                       gAAATTGCCGCCGGTGGGTTCGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGCCCGTGCCGCCGGCCTCCTCGCCCCAATGGACGaa                                            >  4:211451/1‑147 (MQ=255)
                                                                                                             gccgccGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTcgc                                    >  6:32431/1‑149 (MQ=255)
                                                                                                                         ttgggtGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGa                                                >  6:157885/1‑125 (MQ=255)
                                                                                                                         ttgggtGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGa                                                <  5:157885/125‑1 (MQ=255)
                                                                                                                               gtCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTAGCACCAATGGACGAACAGGTCGCGAGCCTGGAGGTCGAggt                  >  3:244167/1‑149 (MQ=255)
                                                                                                                                 cGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtcg                <  6:94323/149‑1 (MQ=255)
                                                                                                                                  gATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtcgg               <  1:36747/149‑1 (MQ=255)
                                                                                                                                    ctgcccTGCCGCCCATCATGACCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtcggt              >  3:185331/6‑148 (MQ=21)
                                                                                                                                     gtgGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtcggt              >  4:240804/1‑147 (MQ=255)
                                                                                                                                     gtgGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAggtcggt              >  4:236521/1‑147 (MQ=255)
                                                                                                                                       ggCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAgg          <  8:275126/149‑1 (MQ=255)
                                                                                                                                           gCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGc        >  5:4333/1‑147 (MQ=255)
                                                                                                                                               gTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAggcg  >  1:41165/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGTTGGTCCATACCACCACTTCCAGCGGGGCTTCGGTGGTCAGGTGCAGGTCGTTGAGCGTGCGCATCACTTCGAGGCCGGCCATGACGCCGTAGCAGCCATCGAACTTGCCGCCGGTGGGTTGGGTGTCGATGTGGCTGCCGGTCATGATGGGCGGCAGGGCAGGGTTGCGCCCTGGGCGGCGGGCGAAGATATTGCCGATGGCGTCGACGCTGACCGTGCAGCCGGCCTCCTCGCACCAATGGACGAACAGGTCGCGAGCCTGGCGGTCGAGGTCGGTCAGGGCCAGGCG  >  NC_002947/720778‑721069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑