Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 146,599 G→T 6.2% L119L (CTG→CTT)  PP_0138 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947146,5990GT 6.2% 206.6 / 3.2 65L119L (CTG→CTT) PP_0138membrane protein
Reads supporting (aligned to +/- strand):  ref base G (28/33);  new base T (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GTCGAGTGGCTGGCCAAGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGGCGCGCCAGAC  >  NC_002947/146453‑146746
                                                                                                                                                  |                                                                                                                                                   
gtcgAGTGGCTGGCCAAGGCGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGaa                                                                                                                                                   <  1:15348/149‑1 (MQ=255)
    aGTGGCTGGCCAAGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGcc                                                                                                                                               <  4:272618/149‑1 (MQ=255)
             ccAAGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCATCCCTGTGCCTGAGCACCATGAACTTCGATGCCAACTGCATGGGACTGGCCAACGCTGCTACGCCGATTGGCCGGAAGGCCATgcgcgct                                                                                                                                      >  7:157025/1‑148 (MQ=255)
              cAAGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATgcgcgcgc                                                                                                                                     >  8:113700/1‑149 (MQ=255)
                aGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTTATCACCATGAACTTCGCTGCCAACGGGCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTg                                                                                                                                   <  4:32187/149‑1 (MQ=255)
                aGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTg                                                                                                                                   <  2:275689/149‑1 (MQ=255)
                 ggTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCTTGGGACTGGACAACGCTGCTACGCCGATTGGCCTTCAGGCCaatcg                                                                                                                                          <  1:239231/141‑5 (MQ=255)
                 ggTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTTCAGGCCaatcg                                                                                                                                          >  2:239231/1‑137 (MQ=255)
                 ggTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTg                                                                                                                                   >  7:71748/1‑148 (MQ=255)
                 ggTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGc                                                                                                                                  <  8:6732/149‑1 (MQ=255)
                  gTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCa                                                                                                                                 <  5:147747/149‑1 (MQ=255)
                   tGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAg                                                                                                                                <  5:135203/149‑1 (MQ=255)
                        ggCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAg                                                                                                                             <  8:21403/147‑1 (MQ=255)
                        ggCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGc                                                                                                                            <  8:71748/148‑1 (MQ=255)
                        ggCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGc                                                                                                                            <  7:40764/148‑1 (MQ=255)
                        ggCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCt                                                                                                                           <  7:113700/149‑1 (MQ=255)
                           ccGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGaa                                                                                                                        >  3:275053/1‑149 (MQ=255)
                            cGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAAc                                                                                                                       >  2:280777/1‑149 (MQ=255)
                                  tCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCgg                                                                                                                  <  1:280777/148‑1 (MQ=255)
                                         ccTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTa                                                                                                          <  2:76843/149‑1 (MQ=255)
                                          cTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCAccag                                                                                                         >  3:109732/1‑146 (MQ=255)
                                           tGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTAcc                                                                                                        >  7:296327/1‑149 (MQ=255)
                                             aTGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACcgc                                                                                                      <  4:275053/149‑1 (MQ=255)
                                                ccGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGa                                                                                                    <  8:191101/148‑1 (MQ=255)
                                                  ggAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGaac                                                                                                  >  3:164018/1‑146 (MQ=255)
                                                        gccgccCGGCCACCCAGCCCAGGGCCAGCTCACCATGAACTTCGCTGCCCACGGCCTGGGACTGGAAAACCCTGCTACGCCGCTTGTCCTGAAGGCCATGCGCGCGCTGCAGGAGCAGAACCCCGGCAGCCCTACCGCCAGCCAcgcg                                                                                            >  4:67814/1‑148 (MQ=255)
                                                         ccgccCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACgcgc                                                                                           <  3:83489/148‑1 (MQ=255)
                                                           gccCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTTCAGGCCaatcg                                                                                                                                          <  6:65720/99‑5 (MQ=255)
                                                           gccCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTTCAGGCCaatcg                                                                                                                                          >  5:65720/1‑95 (MQ=255)
                                                           gccCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGa                                                                                        <  6:254128/149‑1 (MQ=255)
                                                             ccGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAACAACGCGCAGATc                                                                                      <  4:164018/149‑1 (MQ=255)
                                                                gCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATcc                                                                                     >  7:250376/1‑147 (MQ=255)
                                                                gCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATcc                                                                                     <  8:250376/147‑1 (MQ=255)
                                                                   aCCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTc                                                                                >  2:43019/1‑149 (MQ=255)
                                                                     ccAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACACCGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGgca                                                                                                                >  6:51744/1‑115 (MQ=255)
                                                                     ccAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCGGCAGGAGCTGAACCCCGgca                                                                                                                <  5:51744/115‑1 (MQ=255)
                                                                       aGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTCAACCCCGGCAGCACAACCGCGAGCAACGCGCAGATCCTGTACCAgg                                                                            >  6:281998/1‑149 (MQ=255)
                                                                        gCCCTGGGCCTGATCACCATGAACTTCGCTGCCACCGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGt                                                                           <  8:296327/149‑1 (MQ=255)
                                                                           cTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACTCTGATTGGCCTGAAGGCCATGCGCGCGCTGCAg                                                                                                                                <  8:188507/93‑1 (MQ=255)
                                                                           cTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAg                                                                                                                                >  7:188507/1‑93 (MQ=255)
                                                                                   gATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCt                                                                                    >  1:135202/1‑129 (MQ=255)
                                                                                   gATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCt                                                                                    <  2:135202/129‑1 (MQ=255)
                                                                                   gATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCt                                                                >  2:169934/1‑149 (MQ=255)
                                                                                       aCCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCAGAACCCCGCCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCATGGTGCTCAACGCCtcgtc                                                            >  7:304088/1‑149 (MQ=255)
                                                                                        ccATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGc                                                                  >  6:124970/1‑142 (MQ=255)
                                                                                        ccATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGc                                                                  <  5:124970/142‑1 (MQ=255)
                                                                                        ccATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCAGCTACGCCGATTGGCCTGAAGGCCAAGCTCGCGCTGCAGCAGCTGAACCCCGGCAGCACTACCCCGAGCAACGCGCAGATCCTGTTCCAGGTGCTCAACGCCtcgtcg                                                           >  6:173944/1‑149 (MQ=255)
                                                                                         cATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGc                                                          >  3:95568/1‑149 (MQ=255)
                                                                                             aaCTTCGCTGCCTACGGCCTGGGACTGGACATCGCTGCTACGCCGATTGGCCTGAAGGCGATGCGCGCGCTGCTGGAGCTGAACCCCGGCAGCACTACCGCGTGCAACGCGCAGATGCTGTTGCTGGTGCTCAACGCCTCGTCGCTGa                                                       <  5:173944/148‑1 (MQ=255)
                                                                                              aCTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGAcc                                                     <  3:67814/149‑1 (MQ=255)
                                                                                                     tGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCtgctgc                                              >  4:118491/1‑149 (MQ=255)
                                                                                                       ccAACGGCCTGTGACTGGACACCGCTGCTAAGCCGATTGGCCTGAATGACATGCGCGCGCTGCAGGATCTGAACCCCGTCAGCACTACCGAGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACTACTCGTAGCTGACCCtgctgcag                                            <  8:157025/149‑3 (MQ=255)
                                                                                                                tGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGcc                                                                 <  7:274779/119‑1 (MQ=255)
                                                                                                                tGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGcc                                                                 >  8:274779/1‑119 (MQ=255)
                                                                                                                  ggACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTc                                   <  4:95568/147‑1 (MQ=255)
                                                                                                                           aaCGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCg                        >  1:172265/1‑149 (MQ=255)
                                                                                                                                  ctACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCa                  <  1:169934/148‑1 (MQ=255)
                                                                                                                                      gCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGTCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCg                                            <  8:293955/118‑1 (MQ=255)
                                                                                                                                      gCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCATCGTCGCTGACCCTGCTGCCg                                            >  7:293955/1‑118 (MQ=255)
                                                                                                                                               ccTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGgcgcgc       >  4:246943/1‑146 (MQ=255)
                                                                                                                                               ccTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGgcgc         >  1:110141/1‑144 (MQ=255)
                                                                                                                                               ccTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGgcgc         <  2:110141/144‑1 (MQ=255)
                                                                                                                                                 tGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGTTCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGt                                          >  6:50816/1‑109 (MQ=255)
                                                                                                                                                 tGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGTTCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGt                                          <  5:50816/109‑1 (MQ=255)
                                                                                                                                                 tGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGGCGCGCCAGAc  >  4:116370/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTCGAGTGGCTGGCCAAGGTGCTAGGCCCGCTGTTCGCCCGCCTGATGCCGGAGGTGCCGCCCGGCCACCCAGCCCTGGGCCTGATCACCATGAACTTCGCTGCCAACGGCCTGGGACTGGACAACGCTGCTACGCCGATTGGCCTGAAGGCCATGCGCGCGCTGCAGGAGCTGAACCCCGGCAGCACTACCGCGAGCAACGCGCAGATCCTGTTCCTGGTGCTCAACGCCTCGTCGCTGACCCTGCTGCCGGTAACCGTCTTCATGTACCGGGCCCAGCAAGGCGCGCCAGAC  >  NC_002947/146453‑146746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑