Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	790,155	A→T	11.9%	intergenic (+43/+17)	gdhA → / ← PP_0676	NADP‑dependent glutamate dehydrogenase/transcription elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	790,155	0	A	T	11.9%	107.5 / 6.7	42	intergenic (+43/+17)	gdhA/PP_0676	NADP‑dependent glutamate dehydrogenase/transcription elongation factor
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base T (2/3);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

ACTACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCC  >  NC_002947/790009‑790300                                                                                                                                                   |                                                                                                                                                  aCTACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAag                                                                                                                                                 <  1:306260/149‑1 (MQ=255)     cGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAagcagc                                                                                                                                             >  5:182325/1‑149 (MQ=255)         gAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGccc                                                                                                                                           >  4:136672/1‑147 (MQ=255)           agagGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccatc                                                                                                                                        <  3:237100/148‑4 (MQ=255)              ggCTGATGGCCGGATCAAGTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGcc                                                                                                                                    <  6:182325/149‑1 (MQ=255)              ggCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGcc                                                                                                                                    <  3:136672/149‑1 (MQ=255)                        cGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccatc                                                                                                                                        >  7:172347/1‑132 (MQ=255)                        cGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccatc                                                                                                                                        <  8:172347/135‑4 (MQ=255)                         ggATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCg                                                                                                                         <  2:117709/149‑1 (MQ=255)                              aaCTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAggg                                                                                                                                      >  8:262905/1‑131 (MQ=255)                              aaCTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAggg                                                                                                                                      <  7:262905/131‑1 (MQ=255)                               aCTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccat                                                                                                                                         <  1:144529/127‑3 (MQ=255)                               aCTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccat                                                                                                                                         >  2:144529/1‑125 (MQ=255)                                   cGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAAtct                                                                                                               >  7:210108/1‑149 (MQ=255)                                   cGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAAtc                                                                                                                <  7:208370/148‑1 (MQ=255)                                      cAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGCATCTCaa                                                                                                            >  3:13334/1‑149 (MQ=255)                                        aGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGGTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAacc                                                                                                          <  8:210108/149‑1 (MQ=255)                                             gCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACct                                                                                                      >  2:283655/1‑148 (MQ=255)                                                         ggCTTCGTGAAAGTGGCCGATGCTATGCTTGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCGCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTc                                                                                         <  1:57432/149‑1 (MQ=255)                                                          gCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTcc                                                                                        >  5:220542/1‑149 (MQ=255)                                                                  aaaGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACctct                                                                                                    <  4:216252/129‑1 (MQ=255)                                                                  aaaGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACctct                                                                                                    >  3:216252/1‑129 (MQ=255)                                                                  aaaGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCgg                                                                                >  1:72359/1‑149 (MQ=255)                                                                  aaaGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCgg                                                                                <  1:283655/149‑1 (MQ=255)                                                                     gTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAggg                                                                                                                                      <  5:220929/92‑1 (MQ=255)                                                                     gTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAggg                                                                                                                                      >  6:220929/1‑92 (MQ=255)                                                                                  tgcTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGCGGGCCGCTTTGCGTCGCGATGGGCAGCAAAGCACCCCCAGGGCCAACAACCCTCGCCCAGAATCTCAACCACCTATAAAGCCAGTTCACCCACCCGCCGCCGCAAGaccac                                                                 >  4:30336/1‑148 (MQ=255)                                                                                           aGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAg                                                                                                                    >  2:257116/1‑88 (MQ=255)                                                                                           aGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAg                                                                                                                    <  1:257116/88‑1 (MQ=255)                                                                                              gCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGc                                                    <  2:72359/149‑1 (MQ=255)                                                                                                      tgatctgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGaccac                                                                 >  7:3971/1‑128 (MQ=255)                                                                                                      tgatctgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGaccac                                                                 <  8:3971/128‑1 (MQ=255)                                                                                                      tgatctgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGcccc                                            >  4:114728/1‑149 (MQ=255)                                                                                                           tgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCCGCCCCCGCCAGACCACCACCTCCCCCCGCCCAGcccccacc                                        >  6:142470/1‑144 (MQ=255)                                                                                                                 cGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCACAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGcc                                                   >  4:104493/1‑131 (MQ=255)                                                                                                                 cGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCACAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGcc                                                   <  3:104493/131‑1 (MQ=255)                                                                                                                   tAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAgcccgc                               <  6:220542/149‑1 (MQ=255)                                                                                                                                  cGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAAccc                <  1:143975/149‑1 (MQ=255)                                                                                                                                   gTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCCCCGGCCGCCGCCAGACCCCCTCATCCCCCGGCCCACCCCCGAGCAAACCCCGCCCCAATGGCGCAcccc               >  1:287169/1‑149 (MQ=255)                                                                                                                                              cTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAAc    <  4:13334/149‑1 (MQ=255)                                                                                                                                                 cAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAAccc  <  3:30336/148‑1 (MQ=255)                                                                                                                                                 cAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAAcc   <  6:62294/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACTACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCC  >  NC_002947/790009‑790300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑