Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 802,773 G→A 9.9% intergenic (+31/‑55) obg → / → proB GTPase Obg/glutamate 5‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947802,7730GA 9.9% 132.7 / 5.2 53intergenic (+31/‑55)obg/proBGTPase Obg/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base A (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

GTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTC  >  NC_002947/802627‑802908
                                                                                                                                                  |                                                                                                                                       
gTACCCTGCGTCGTACCGGCGTCAAGAGCGCGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGa                                                                                                                                       <  8:68887/149‑1 (MQ=255)
     cTGCGTCGTACCGGCGGCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAgcggc                                                                                                                                  <  3:216228/149‑1 (MQ=255)
          gcgtACCGGCGTCACCAGCGTGCATGACATCGGCGCCTACGATGGTTGGGACGATGATTTCGAGCACGACCAACCCGGCCCGTAAATAATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCCGCGCGCGGCGttta                                                                                                                             <  4:108808/148‑2 (MQ=255)
            gtACCGGCTTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGtttagt                                                                                                                           <  8:253076/149‑4 (MQ=255)
                cGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCTGCGTTTTTGTATc                                                                                                                       >  3:119825/1‑149 (MQ=255)
                        agagCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTa                                                                                                                >  1:257655/1‑148 (MQ=255)
                         gagCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATc                                                                                                              <  2:73920/149‑1 (MQ=255)
                               gCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCGATCTCGAGCGGCGTTTTTGTATCCACCGTATCTa                                                                                                            >  8:292252/1‑145 (MQ=255)
                                 aTGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCTGCGTTTTTGTATCCACGGTATCTACAGAtt                                                                                                      <  4:119825/149‑1 (MQ=255)
                                    aCATCGGGGACGACGATGGTTGGGACTATGATTTCGAGGACGACGTAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGttt                                                                                                                              <  4:191485/122‑1 (MQ=255)
                                    aCATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCCAGAGAGCGGCGttt                                                                                                                              >  3:191485/1‑122 (MQ=255)
                                                    tGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaagaa                                                                                    >  8:283797/1‑148 (MQ=255)
                                                     ggTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGtt                                                                                           >  8:186175/1‑140 (MQ=255)
                                                     ggTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGtt                                                                                           <  7:186175/140‑1 (MQ=255)
                                                          tgACGATGATTTAGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGa                                                                             <  7:292252/148‑1 (MQ=255)
                                                           gACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGaa                                                                            <  7:11952/149‑1 (MQ=255)
                                                              gatgatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaagaa                                                                                    >  4:87143/1‑138 (MQ=255)
                                                              gatgatTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGgaagaa                                                                                    <  3:87143/138‑1 (MQ=255)
                                                                   tttCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACa                                                                                                 >  5:20614/1‑120 (MQ=255)
                                                                   tttCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACa                                                                                                 <  6:20614/120‑1 (MQ=255)
                                                                             gacgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTgg                                                                                         >  1:138387/1‑118 (MQ=255)
                                                                             gacgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTgg                                                                                         <  2:138387/118‑1 (MQ=255)
                                                                               cgaAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAg                                                        >  1:25221/1‑149 (MQ=255)
                                                                               cgaAGACGGGCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAg                                                        <  8:193760/149‑1 (MQ=255)
                                                                                 aaGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAgcg                                                      >  3:185530/1‑149 (MQ=255)
                                                                                   gACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCt                                                    >  7:152209/1‑149 (MQ=255)
                                                                                      ggTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTggg                                                 >  2:177616/1‑149 (MQ=255)
                                                                                       gTCCGGAAACCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGt                                                >  2:270941/1‑149 (MQ=255)
                                                                                           ggAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGt                                             >  7:99332/1‑148 (MQ=255)
                                                                                                  aTTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGAtt                                      <  2:25221/148‑1 (MQ=255)
                                                                                                     tACGTGCGCGACTGATAGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGc                                   <  8:76042/148‑1 (MQ=255)
                                                                                                            gcgACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGTGATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCTCCAACCGGGGGGTCGTTAAGCTTGGCAGTGctct                           >  8:233225/1‑149 (MQ=255)
                                                                                                             cgACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctg                          <  4:185530/149‑1 (MQ=255)
                                                                                                              gACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAg                                                        <  2:175449/118‑1 (MQ=255)
                                                                                                                 tGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctgct                        <  2:186446/147‑1 (MQ=255)
                                                                                                                 tGATCGGTTGCAGTACACTAAACGCCGCACTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTctgct                        >  1:186446/1‑147 (MQ=255)
                                                                                                                   aTCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTAGCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGAc                     <  7:283797/148‑1 (MQ=255)
                                                                                                                    tCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATACACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGAccg                   >  8:257462/1‑149 (MQ=255)
                                                                                                                       gTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCAAGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGAAGTGCTCTGCTGAcagcc                 >  2:128703/1‑148 (MQ=255)
                                                                                                                        ttGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGa               <  2:257655/149‑1 (MQ=255)
                                                                                                                          gCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGt                                <  2:184067/130‑1 (MQ=255)
                                                                                                                          gCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGt                                >  1:184067/1‑130 (MQ=255)
                                                                                                                          gCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGAt              <  1:191693/148‑1 (MQ=255)
                                                                                                                                cacTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAgg       <  8:99332/149‑1 (MQ=255)
                                                                                                                                cacTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAgg       >  5:152570/1‑149 (MQ=255)
                                                                                                                                  aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTggg                                                 <  7:39638/103‑1 (MQ=39)
                                                                                                                                  aaaaaCGCCGCTCCAGAGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTggg                                                 >  8:39638/3‑105 (MQ=39)
                                                                                                                                   tAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGcc    >  6:76711/1‑149 (MQ=255)
                                                                                                                                   tAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGATCTGCTGACCGCCGATGGCAAGGGc     >  8:157414/1‑148 (MQ=255)
                                                                                                                                     aaCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGcc    <  1:177616/147‑1 (MQ=255)
                                                                                                                                     aaCGCCGCTCTCTGGAGCGGCGGTTTTGTATCCCCGGTATCGACAGAGTCGACATAGGGTGGAATAAGATGCGAAGAAAGGTGCCGGGCGCCCCGCGCTGTGGCGTTAAGATTGGCAGTGGTATGCTGACCGCCCATGCGAAGGGCCTc  >  6:222806/1‑149 (MQ=255)
                                                                                                                                      aCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACAt                                                                                                <  7:90247/54‑1 (MQ=255)
                                                                                                                                      aCGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACAt                                                                                                >  8:90247/1‑54 (MQ=255)
                                                                                                                                                  |                                                                                                                                       
GTACCCTGCGTCGTACCGGCGTCAAGAGCGTGCATGACATCGGCGACGACGATGGTTGGGACGATGATTTCGAGGACGACGAAGACGGTCCGGAAATCATTTACGTGCGCGACTGATCGGTTGCAGTACACTAAACGCCGCTCTCTGGAGCGGCGTTTTTGTATCCACGGTATCTACAGATTCGACATAGGTTGGAAGAAGATGCGAAGCAAGGTGACGGGCGCCAAGCGCTGGGTCGTTAAGATTGGCAGTGCTCTGCTGACCGCCGATGGCAAGGGCCTC  >  NC_002947/802627‑802908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑