| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 826,700 | G→C | 13.9% | intergenic (‑102/‑110) | PP_0710 ← / → ycaC‑I | hypothetical protein/putative hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 826,700 | 0 | G | C | 13.9% | 89.9 / 8.2 | 36 | intergenic (‑102/‑110) | PP_0710/ycaC‑I | hypothetical protein/putative hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (17/14); new base C (2/3); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
TCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAG > NC_002947/826561‑826839 | gcggcggcggCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGGGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGAt < 4:55696/148‑1 (MQ=255) ggcggcggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATgg < 4:160644/149‑1 (MQ=255) gcggcggcTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATggg > 6:111353/1‑149 (MQ=255) tGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAggg > 8:104339/1‑148 (MQ=255) ccGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAATTAATCAGTTCACAAATTTCTGAACTGa > 6:219942/1‑134 (MQ=255) ccGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCACAAATTTCTGAACTGa < 5:219942/134‑1 (MQ=255) cTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAg < 5:55331/149‑1 (MQ=255) ttGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAg > 5:322384/1‑148 (MQ=255) gAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAg < 6:322384/146‑1 (MQ=255) gAAGTGTCCGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGc > 4:204721/1‑148 (MQ=255) aaGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATgggg < 1:275268/130‑1 (MQ=255) aaGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATgggg > 2:275268/1‑130 (MQ=255) aaGTGTCCGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCaa < 3:204721/149‑1 (MQ=255) gggAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGtct < 5:111353/149‑1 (MQ=255) ggCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCa > 1:305726/1‑149 (MQ=255) gCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCAc > 8:289568/1‑149 (MQ=255) cTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCTGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGtctctc < 1:307783/143‑1 (MQ=255) cTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCGGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGtctctc > 2:307783/1‑143 (MQ=255) aTTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGc < 7:289568/149‑1 (MQ=255) aTTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGc > 2:121936/1‑149 (MQ=255) gtgAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGTCAGTACAGTCAAGCCGACTCACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGAAAc > 4:177537/1‑149 (MQ=255) aaGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTc < 1:121936/148‑1 (MQ=255) gCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTccc > 8:79715/1‑148 (MQ=255) ttgtggCGTTGGTCGAGGGGAATAATGGCTTGCTTCAAATATTCAGTTCAGATATTTGTGAACTGATGTGGCACAGGTCAGTACAGGCAAGCCGACTGACTGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGc < 3:177537/146‑1 (MQ=255) tggtggCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCt > 6:197071/1‑149 (MQ=255) tggCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTg > 3:135331/1‑147 (MQ=255) tggCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTg > 3:130414/1‑147 (MQ=255) ggTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCaa < 7:104339/149‑1 (MQ=255) gTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAg < 3:312416/149‑1 (MQ=255) ggggAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCa > 1:206223/1‑149 (MQ=255) gggAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCAc > 5:83357/1‑149 (MQ=255) gCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTc < 3:225324/149‑1 (MQ=255) tCAGTTCAGAAATTTGTGAAGTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTg < 7:124245/149‑1 (MQ=255) tCAGTTCACAAATTTCTGAACTGAT‑GGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTg > 6:204578/1‑148 (MQ=255) ttCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGAc > 7:271840/1‑148 (MQ=255) cacaaaTTTCTGAACTGAT‑GGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAg < 5:204578/145‑1 (MQ=255) | TCGGCGGCGGCTTGCCGCACTTGAAGTGTCTGGTCCACGGGAGTGGCCTCACGGTTTTTTTGTGATTGTGGTGAAGCGATTCTGGTGGCGGTGGGCGAGGGGAAAAATCGCTGGCGGCAAATAATCAGTTCAGAAATTTGTGAACTGATGGGGCACAGGGCAGTACAGGCAAGCCGACTGACGGTCTCTCCCACCCTCCATCCTTGCCTACGCTGATACTCCCAACCCGCTGAAGCAAGGAGATCCACTGTGGCCTTCCAATACAAGCGTCTGGACAAG > NC_002947/826561‑826839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |