| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 863,898 | T→A | 12.2% | intergenic (+15/+21) | hemH → / ← uraA | ferrochelatase/uracil permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 863,898 | 0 | T | A | 12.2% | 113.2 / 6.4 | 48 | intergenic (+15/+21) | hemH/uraA | ferrochelatase/uracil permease |
| Reads supporting (aligned to +/- strand): ref base T (23/19); new base A (4/2); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGCC > NC_002947/863755‑864044 | gAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCg < 7:162491/149‑1 (MQ=255)gAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCg > 5:8413/1‑149 (MQ=255) aaGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCgg > 5:200835/1‑149 (MQ=255) gagaTAGGCCTGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCGGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGc > 7:188954/1‑148 (MQ=255) cATGCGTGGCCGGGAGCTGTTTGTCGAGGGGGGCGGCCTGTCGCTGGGGCTGGTGCCGTGCCTGAATGATCACCCGTAGTGGGTTAGGGTGTTGGCTTATATGTGTGAAACGGCCTGAATCCATGGGGCCGCTTTGCGGCCCCTTCCGa < 6:200835/149‑1 (MQ=255) tGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTc > 2:190927/1‑144 (MQ=255) tGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCAAAGCGGCCCCTTc < 1:190927/144‑1 (MQ=255) gTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTCGCGGCCCCTTCCGACCGGTCATACATCCAGcttctt > 8:151324/1‑149 (MQ=255) gAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTtgtg > 3:256816/1‑149 (MQ=255) gggcggCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTcc < 2:102526/149‑1 (MQ=255) gggcggCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTCGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTcc < 7:151324/149‑1 (MQ=255) ccAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGt < 3:87771/149‑1 (MQ=255) tggtgctggtgcCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTAccc > 7:229032/1‑149 (MQ=255) gctggtgcCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCgg > 5:199613/1‑147 (MQ=255) tggtgcGGTGCCTTAATGAGCACCCGGAGTGGGTGAGGTGGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGGTTCCAGCTGTCATTACCCGGCAGa < 6:8413/149‑1 (MQ=255) ccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCa < 8:229032/148‑1 (MQ=255) ccgtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAg > 4:201061/1‑149 (MQ=255) gtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCa < 5:132170/140‑1 (MQ=255) gtgccTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCa > 6:132170/1‑140 (MQ=255) tCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATAGAAGGGGCCGCTTTGCGGCCCCTTCCGAACGGTCATACATACAGCTTCTTGTGCTTCCAGATGTAATTACACGGCAGAATCAGGTTCAGGGCAATAg > 5:227983/1‑149 (MQ=255) aCCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGc > 3:214265/1‑148 (MQ=255) agtttctGAGTGTGCTGGCTGATATGTTTGAACAGTCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATg < 6:227983/143‑1 (MQ=255) gTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGca > 8:147746/1‑149 (MQ=255) gtgtTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGc > 7:296352/1‑149 (MQ=255) gtgtTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGATTCTTGTGATTCCAGCTGTCATTACCCGGCAGAATAAGTTTCAGGGCAATAGCCACAATGCCGCACAggtc > 8:303199/1‑147 (MQ=255) gCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTGATACATACAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGACACAATGGCGCAAAGGGCGATGca > 3:14787/1‑148 (MQ=255) cTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGccc > 4:285040/1‑149 (MQ=255) tGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCt > 1:265769/1‑149 (MQ=255) tGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCATTGCGGCCCCTTACGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGACACAATGGCGCACAGGGCGATGCCCt > 5:172940/1‑149 (MQ=255) gATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCtt > 6:140184/1‑149 (MQ=255) tgtgttAATAGGTCTGTATCGAAGGGGCCGCTTTGCGGCCCCTTACGACCGGTGATAAATACAGCTTCTTGTGCTTCCAGCTGGAATTACCCGGCAGAATCAGGTTCAGTTCAATATGCACAATGGCGAACAGGGCGATGCCCTTCAg < 8:188954/148‑1 (MQ=255) tgAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGcccc < 3:201061/149‑1 (MQ=255) aaaaGGCCTGAATCGAAGGTGCCGCTTTGCGGCCCCTTCCGACCGGTCATACTTCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCa < 6:172940/148‑1 (MQ=255) aaaaGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGTTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCa < 2:265769/148‑1 (MQ=255) aaaaGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCa < 6:116184/148‑1 (MQ=255) aaaGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGGCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAg > 6:73256/1‑148 (MQ=255) gCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATc < 4:214265/149‑1 (MQ=255) aaTCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCgg < 6:199613/147‑1 (MQ=255) gAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCa > 1:288372/1‑138 (MQ=255) gAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCa < 2:288372/138‑1 (MQ=255) gAAGGGGCCGCAAAGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATg < 5:252942/120‑1 (MQ=255) gAAGGGGCCGCAAAGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCATCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATg > 6:252942/1‑120 (MQ=255) aaGGGGCCGCAAAGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCa > 6:263243/1‑149 (MQ=255) ggggCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCAcc < 4:256816/149‑1 (MQ=255) ggggCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCAcc > 2:225026/1‑149 (MQ=255) gggCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCg < 5:140184/149‑1 (MQ=255) cGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGc > 7:283386/1‑149 (MQ=255) gCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGcc > 4:141043/1‑149 (MQ=255) | GAAGAGATAGGCATGCGTGGCAGCGAGCAGTTTGTCGAGGCGGGCGGCCAGGAGCTGGTGCTGGTGCCGTGCCTGAATGATCACCCGGAGTGGGTGAGGGTGTTGGCTGATATGTGTGAAAAGGCCTGAATCGAAGGGGCCGCTTTGCGGCCCCTTCCGACCGGTCATACATCCAGCTTCTTGTGCTTCCAGCTGTCATTACCCGGCAGAATCAGGTTCAGGGCAATAGCCACAATGGCGCACAGGGCGATGCCCTTCAGGCCCCAGTCATCCGGGCCGTCACCGCTGCC > NC_002947/863755‑864044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |