Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 995,254 T→C 8.3% D781G (GAT→GGT)  PP_0860 ← sulfite reductase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947995,2540TC 8.3% 130.6 / 2.3 48D781G (GAT→GGT) PP_0860sulfite reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (26/18);  new base C (2/2);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

ACCCCCGCTGCCATGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGACCCGG  >  NC_002947/995131‑995394
                                                                                                                           |                                                                                                                                            
aCCCCCGCTGCCATGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGACCGCGCGAGAACGCCAGGTCCAACCg                                                                                                                     >  8:27973/1‑149 (MQ=255)
aCCCCCGCTGCCATGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGACCGCGCGAGAACGCCAGGTCCAACCg                                                                                                                     >  8:22961/1‑149 (MQ=255)
            aTGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGcc                                                                                                         >  8:47832/1‑149 (MQ=255)
             tGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGACCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCg                                                                                                        <  7:27973/149‑1 (MQ=255)
             tGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGACCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCg                                                                                                        <  7:22961/149‑1 (MQ=255)
               ccTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCg                                                                                                        >  1:155733/1‑147 (MQ=255)
               ccTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCg                                                                                                        <  2:155733/147‑1 (MQ=255)
               cattGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGtt                                                                                                      <  6:229669/147‑1 (MQ=255)
                   ggAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGAt                                                                                                                                              >  6:144781/1‑105 (MQ=255)
                   ggAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGAt                                                                                                                                              <  5:144781/105‑1 (MQ=255)
                    gAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCa                                                                                                 <  1:276800/149‑1 (MQ=255)
                    gAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCa                                                                                                 <  8:246203/149‑1 (MQ=255)
                    gAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCa                                                                                                 <  7:47832/149‑1 (MQ=255)
                                   aTACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAg                                                                                    >  3:133660/1‑147 (MQ=255)
                                   aTACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAg                                                                                    <  4:133660/147‑1 (MQ=255)
                                     acacGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCa                                                                                >  8:255688/1‑149 (MQ=255)
                                      cacGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGACGCCGTTGTCCAGCCAAGCTTGCAGCTCAt                                                                               >  7:73829/1‑149 (MQ=255)
                                             gCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCcaca                                                                        >  5:216133/1‑149 (MQ=255)
                                                           ccAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGtt                                                                                                      <  7:278662/105‑1 (MQ=255)
                                                           ccAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGtt                                                                                                      >  8:278662/1‑105 (MQ=255)
                                                                  ttGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCa                                                                                     <  7:237550/115‑1 (MQ=255)
                                                                  ttGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCa                                                                                     >  8:237550/1‑115 (MQ=255)
                                                                  ttGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGccc                                                       >  7:41448/1‑145 (MQ=255)
                                                                  ttGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGccc                                                       <  8:41448/145‑1 (MQ=255)
                                                                    gAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCaa                                                                                            <  2:73527/106‑1 (MQ=255)
                                                                    gAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCaa                                                                                            >  1:73527/1‑106 (MQ=255)
                                                                    gAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTAc                                                 >  7:65190/1‑149 (MQ=255)
                                                                    gAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTAc                                                 >  3:8143/1‑149 (MQ=255)
                                                                        tCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCAAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCt                                              >  5:223183/1‑148 (MQ=255)
                                                                          cTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCAc                                           >  1:70445/1‑149 (MQ=255)
                                                                          cTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCAc                                           >  6:88047/1‑149 (MQ=255)
                                                                          cTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCAc                                           >  3:258240/1‑149 (MQ=255)
                                                                                  gCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCGCGATTACGCTGCCCGAAcag                                    >  6:267263/1‑148 (MQ=255)
                                                                                                 ccTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCgctgc                    >  7:101285/1‑149 (MQ=255)
                                                                                                     cacaTACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCAc                <  2:139095/149‑1 (MQ=255)
                                                                                                        aTACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCt                                                                                         >  7:252635/1‑73 (MQ=255)
                                                                                                        aTACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCt                                                                                         <  8:252635/73‑1 (MQ=255)
                                                                                                        aTACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAAcagca                                  <  6:149571/128‑1 (MQ=255)
                                                                                                        aTACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAAcagca                                  >  5:149571/1‑128 (MQ=255)
                                                                                                         tACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCgg             >  3:203916/1‑148 (MQ=255)
                                                                                                         tACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCgg             >  3:207984/1‑148 (MQ=255)
                                                                                                           cacCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCa           <  5:88047/148‑1 (MQ=255)
                                                                                                              cTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGa       <  4:207984/149‑1 (MQ=255)
                                                                                                              cTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGa       <  4:203916/149‑1 (MQ=255)
                                                                                                                tCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGAcc     >  1:85079/1‑149 (MQ=255)
                                                                                                                 cccGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAg                                                                                    <  3:80369/69‑1 (MQ=255)
                                                                                                                 cccGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAg                                                                                    >  4:80369/1‑69 (MQ=255)
                                                                                                                   cGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGACCCgg  >  1:135225/1‑149 (MQ=255)
                                                                                                                   cGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGACCCgg  >  8:263356/1‑149 (MQ=255)
                                                                                                                   cGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGACCCgg  <  2:135225/149‑1 (MQ=255)
                                                                                                                           |                                                                                                                                            
ACCCCCGCTGCCATGCCTTGGAGGCTGCCGCAGACATACACGCAAGCCCCGTCGGCCACCCAGCGCTTGAAGTCCTCGGCCTGCTCCAGCATCACGTCCTGCACATACACCTTCCCGGCCTGATCGCGCGAGAACGCCAGGTCCAACCGTGCCAGGTCGCCGTTGTCCAGCCAAGCTTGCAGCTCATCGCCACACAGCAAGTCATGCGCCCGATTACGCTCACCGAACAGCAGCCAGTTGCGCTGCTCACCGGCATTGACCCGG  >  NC_002947/995131‑995394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑