Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,027,996 G→C 6.4% A248A (GCC→GCG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,027,9960GC 6.4% 183.0 / 3.5 63A248A (GCC→GCG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base C (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

TCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGAT  >  NC_002947/1027848‑1028142
                                                                                                                                                    |                                                                                                                                                    
tCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAg                                                                                                                                                      <  8:61069/149‑1 (MQ=255)
    gTTGTAGGCGATATAGCCGAGGTTGAACCCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACACGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAg                                                                                                                                                      <  6:11161/145‑1 (MQ=255)
          ggCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                                            <  1:179088/149‑2 (MQ=255)
                ataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGTTTCTTGTCGGCTTTTAGCTGCTCGATGTGGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                                       <  1:182311/148‑1 (MQ=255)
                 taGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGc                                                                                                                                     <  3:153124/149‑1 (MQ=255)
                    ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                  <  4:219501/149‑1 (MQ=255)
                    ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                    >  8:93934/1‑147 (MQ=255)
                    ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGCGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                    >  8:262850/1‑147 (MQ=255)
                     ctaGGTTGACAACTGCATGCTTCGGCATTTGCAGGTTCTTGTCGTCTTTTAGAGGATCTATGTCGGCCGTGCGTGGGAACAGGGTGACCTGGAACTCTTTCTTCTTGAGCTTCTTCATGCTCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                  <  4:77702/146‑1 (MQ=255)
                     cGAGGTTGAAACCGGCCTGCTGCGGCATTTGGAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑g                                                                                                                                <  7:43806/149‑1 (MQ=255)
                         gTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GAt                                                                                                                              >  8:265254/1‑147 (MQ=255)
                         gTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCa                                                                                                                            >  4:202146/1‑149 (MQ=255)
                          ttGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAg                                                                                                                           <  5:291930/149‑1 (MQ=255)
                             aaaCCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGTGTGACCTGGCACTCTTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGtt                                                                                                                        <  3:202146/149‑1 (MQ=255)
                                ccGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTc                                                                                                                    <  2:191434/149‑1 (MQ=255)
                                    ccTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATc                                                                                                                <  5:325799/149‑1 (MQ=255)
                                     ctgctgCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCt                                                                                                               >  3:17340/1‑149 (MQ=255)
                                       gctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCtt                                                                                                              >  2:307388/1‑148 (MQ=255)
                                       gctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCtt                                                                                                              >  5:198951/1‑148 (MQ=255)
                                        ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTc                                                                                                             <  5:213671/148‑1 (MQ=255)
                                        ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGCGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTc                                                                                                             <  7:262850/148‑1 (MQ=255)
                                                   gCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACTTCCTCGGGc                                                                                                 >  2:330404/1‑149 (MQ=255)
                                                      ggTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGc                                                                                              <  6:198951/149‑1 (MQ=255)
                                                              tCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTc                                                                                      >  5:160021/1‑149 (MQ=255)
                                                                ggCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                     <  4:17340/148‑1 (MQ=255)
                                                                ggCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                                    >  3:94446/1‑149 (MQ=255)
                                                                  cTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttg                                                                                  <  7:93934/149‑1 (MQ=255)
                                                                   ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                 >  5:197082/1‑149 (MQ=255)
                                                                   ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                 >  1:99801/1‑149 (MQ=255)
                                                                   ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGCGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                 >  1:45544/1‑149 (MQ=255)
                                                                       aGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                             >  4:153992/1‑149 (MQ=255)
                                                                        gCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                             <  6:160021/148‑1 (MQ=255)
                                                                               gATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGc                                                                     <  2:329077/149‑1 (MQ=255)
                                                                                     ggCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                     <  1:246462/127‑1 (MQ=255)
                                                                                     ggCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCt                                                               >  8:225441/1‑149 (MQ=255)
                                                                                       ccGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                             <  1:307388/149‑1 (MQ=255)
                                                                                          ggCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATGAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGcgcg                                                          >  5:126770/1‑149 (MQ=255)
                                                                                             gTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTcc                                                       <  7:265254/149‑1 (MQ=255)
                                                                                              tGGGAACAGGGTGACCTGGCACTCTTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCt                                                      <  6:197082/149‑1 (MQ=255)
                                                                                              tGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCt                                                      >  6:247374/1‑149 (MQ=255)
                                                                                                ggAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGTTCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTc                                                    <  5:291741/149‑1 (MQ=255)
                                                                                                   aCAGGGTGACCTGGCACTCGTTGTTATTGAGCATCTGCATGCGCACAGAGGCGTCGGTGGTGATGGAAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCATTGTAGCCATTGAAGCGGATCTgc                                                             >  7:50045/1‑137 (MQ=255)
                                                                                                   aCAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                             <  8:50045/137‑1 (MQ=255)
                                                                                                          gACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGc                                          <  7:225441/149‑1 (MQ=255)
                                                                                                             cTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTg                                        >  3:231811/1‑148 (MQ=255)
                                                                                                                  aCTCGTTCTTCTTTAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTG‑GTCGATCTTCACGTCCTCGGGCTGCCAGTTGTCCTTGTGGCCCTTGAAGCGGCTCTGCGGGTCGTTCTGGTATTTGCTGAacac                                   >  8:90985/1‑148 (MQ=255)
                                                                                                                  aCTCGTTCTTCTTGAGCTTCTGCATGCGCACCGTGGCGTCGGTGGTGATGGCAAA‑GATCAGGTTGGACGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAacac                                   >  1:98791/1‑149 (MQ=255)
                                                                                                                      gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                                         >  3:24114/1‑138 (MQ=255)
                                                                                                                      gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                                         <  4:24114/138‑1 (MQ=255)
                                                                                                                          ttcttGAGCTTCTGCATGCGCACCGAGGCGTCGGTTGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCTGATCTGCGGGTCCTTCTGGTATTTGCTGAACACGAACGGGcc                          <  1:330404/149‑1 (MQ=255)
                                                                                                                               gAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCATTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGc                     >  1:107919/1‑149 (MQ=255)
                                                                                                                                   ttCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGAt                 >  4:5809/1‑149 (MQ=255)
                                                                                                                                     cTGCATGCGCACCGTGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc                <  2:98791/148‑1 (MQ=255)
                                                                                                                                        cATGCGCACCGAGGCGTTGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCt            >  7:37102/1‑149 (MQ=255)
                                                                                                                                        cATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCt            >  3:138652/1‑149 (MQ=255)
                                                                                                                                          tGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTc          <  3:5809/149‑1 (MQ=255)
                                                                                                                                           gcgcACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCt         >  5:183850/1‑149 (MQ=255)
                                                                                                                                            cgcACCGAGGCGGCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCCGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTg        <  4:208259/149‑1 (MQ=255)
                                                                                                                                            accACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTg        >  1:67957/3‑149 (MQ=255)
                                                                                                                                             ccACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTgg       >  4:53055/2‑149 (MQ=255)
                                                                                                                                              cACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt      <  2:67957/149‑1 (MQ=255)
                                                                                                                                               aCCGAGGCGTCGGTGGTGATGGCAAAGGATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTACTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt      >  1:3667/1‑149 (MQ=255)
                                                                                                                                               aCCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGtt     >  2:99940/1‑149 (MQ=255)
                                                                                                                                                 cGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGAATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCtcg       >  1:296349/1‑143 (MQ=255)
                                                                                                                                                  gAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGTGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGAt  <  1:99940/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCACGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGAT  >  NC_002947/1027848‑1028142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑