Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,027,999 G→C 6.3% D247E (GAC→GAG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,027,9990GC 6.3% 202.5 / 3.5 63D247E (GAC→GAG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base C (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

GGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGG  >  NC_002947/1027858‑1028147
                                                                                                                                             |                                                                                                                                                      
ggCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                                                 <  1:179088/149‑2 (MQ=255)
      ataGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGTTTCTTGTCGGCTTTTAGCTGCTCGATGTGGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATg                                                                                                                                            <  1:182311/148‑1 (MQ=255)
       taGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGc                                                                                                                                          <  3:153124/149‑1 (MQ=255)
          ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                       <  4:219501/149‑1 (MQ=255)
          ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                         >  8:93934/1‑147 (MQ=255)
          ccGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGCGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                         >  8:262850/1‑147 (MQ=255)
           ctaGGTTGACAACTGCATGCTTCGGCATTTGCAGGTTCTTGTCGTCTTTTAGAGGATCTATGTCGGCCGTGCGTGGGAACAGGGTGACCTGGAACTCTTTCTTCTTGAGCTTCTTCATGCTCACCGAGGCGTCGGTGGTGATGGCaaa                                                                                                                                       <  4:77702/146‑1 (MQ=255)
           cGAGGTTGAAACCGGCCTGCTGCGGCATTTGGAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑g                                                                                                                                     <  7:43806/149‑1 (MQ=255)
               gTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GAt                                                                                                                                   >  8:265254/1‑147 (MQ=255)
               gTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCa                                                                                                                                 >  4:202146/1‑149 (MQ=255)
                ttGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAg                                                                                                                                <  5:291930/149‑1 (MQ=255)
                   aaaCCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGTGTGACCTGGCACTCTTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGtt                                                                                                                             <  3:202146/149‑1 (MQ=255)
                      ccGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTc                                                                                                                         <  2:191434/149‑1 (MQ=255)
                          ccTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATc                                                                                                                     <  5:325799/149‑1 (MQ=255)
                           ctgctgCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCt                                                                                                                    >  3:17340/1‑149 (MQ=255)
                             gctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCtt                                                                                                                   >  2:307388/1‑148 (MQ=255)
                             gctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCtt                                                                                                                   >  5:198951/1‑148 (MQ=255)
                              ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTc                                                                                                                  <  5:213671/148‑1 (MQ=255)
                              ctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGCGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTc                                                                                                                  <  7:262850/148‑1 (MQ=255)
                                         gCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACTTCCTCGGGc                                                                                                      >  2:330404/1‑149 (MQ=255)
                                            ggTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGc                                                                                                   <  6:198951/149‑1 (MQ=255)
                                                    tCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTc                                                                                           >  5:160021/1‑149 (MQ=255)
                                                      ggCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                          <  4:17340/148‑1 (MQ=255)
                                                      ggCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCt                                                                                         >  3:94446/1‑149 (MQ=255)
                                                        cTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttg                                                                                       <  7:93934/149‑1 (MQ=255)
                                                         ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                      >  5:197082/1‑149 (MQ=255)
                                                         ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                      >  1:99801/1‑149 (MQ=255)
                                                         ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGCGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                      >  1:45544/1‑149 (MQ=255)
                                                             aGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                                  >  4:153992/1‑149 (MQ=255)
                                                              gCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                                  <  6:160021/148‑1 (MQ=255)
                                                                     gATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGc                                                                          <  2:329077/149‑1 (MQ=255)
                                                                           ggCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                          <  1:246462/127‑1 (MQ=255)
                                                                           ggCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCt                                                                    >  8:225441/1‑149 (MQ=255)
                                                                             ccGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                                  <  1:307388/149‑1 (MQ=255)
                                                                                ggCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATGAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGcgcg                                                               >  5:126770/1‑149 (MQ=255)
                                                                                   gTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTcc                                                            <  7:265254/149‑1 (MQ=255)
                                                                                    tGGGAACAGGGTGACCTGGCACTCTTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCt                                                           <  6:197082/149‑1 (MQ=255)
                                                                                    tGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCt                                                           >  6:247374/1‑149 (MQ=255)
                                                                                      ggAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGTTCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTc                                                         <  5:291741/149‑1 (MQ=255)
                                                                                         aCAGGGTGACCTGGCACTCGTTGTTATTGAGCATCTGCATGCGCACAGAGGCGTCGGTGGTGATGGAAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCATTGTAGCCATTGAAGCGGATCTgc                                                                  >  7:50045/1‑137 (MQ=255)
                                                                                         aCAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                                  <  8:50045/137‑1 (MQ=255)
                                                                                                gACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGc                                               <  7:225441/149‑1 (MQ=255)
                                                                                                   cTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTg                                             >  3:231811/1‑148 (MQ=255)
                                                                                                        aCTCGTTCTTCTTTAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTG‑GTCGATCTTCACGTCCTCGGGCTGCCAGTTGTCCTTGTGGCCCTTGAAGCGGCTCTGCGGGTCGTTCTGGTATTTGCTGAacac                                        >  8:90985/1‑148 (MQ=255)
                                                                                                        aCTCGTTCTTCTTGAGCTTCTGCATGCGCACCGTGGCGTCGGTGGTGATGGCAAA‑GATCAGGTTGGACGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAacac                                        >  1:98791/1‑149 (MQ=255)
                                                                                                            gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                                              <  4:24114/138‑1 (MQ=255)
                                                                                                            gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCt                                              >  3:24114/1‑138 (MQ=255)
                                                                                                                ttcttGAGCTTCTGCATGCGCACCGAGGCGTCGGTTGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCTGATCTGCGGGTCCTTCTGGTATTTGCTGAACACGAACGGGcc                               <  1:330404/149‑1 (MQ=255)
                                                                                                                     gAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCATTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGc                          >  1:107919/1‑149 (MQ=255)
                                                                                                                         ttCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGAt                      >  4:5809/1‑149 (MQ=255)
                                                                                                                           cTGCATGCGCACCGTGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc                     <  2:98791/148‑1 (MQ=255)
                                                                                                                              cATGCGCACCGAGGCGTTGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCt                 >  7:37102/1‑149 (MQ=255)
                                                                                                                              cATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCt                 >  3:138652/1‑149 (MQ=255)
                                                                                                                                tGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTc               <  3:5809/149‑1 (MQ=255)
                                                                                                                                 gcgcACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCt              >  5:183850/1‑149 (MQ=255)
                                                                                                                                  cgcACCGAGGCGGCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCCGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTg             <  4:208259/149‑1 (MQ=255)
                                                                                                                                  accACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTg             >  1:67957/3‑149 (MQ=255)
                                                                                                                                   ccACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTgg            >  4:53055/2‑149 (MQ=255)
                                                                                                                                    cACCGACGCCTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt           <  2:67957/149‑1 (MQ=255)
                                                                                                                                     aCCGAGGCGTCGGTGGTGATGGCAAAGGATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTACTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt           >  1:3667/1‑149 (MQ=255)
                                                                                                                                     aCCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGtt          >  2:99940/1‑149 (MQ=255)
                                                                                                                                       cGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGAATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCtcg            >  1:296349/1‑143 (MQ=255)
                                                                                                                                        gAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGTGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGAt       <  1:99940/149‑1 (MQ=255)
                                                                                                                                             gTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTAttt                                                 >  2:325083/1‑102 (MQ=255)
                                                                                                                                             gTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTAttt                                                 <  1:325083/102‑1 (MQ=255)
                                                                                                                                             gTCGGTGGTGAAGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTcgg  >  2:144738/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                      
GGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAA‑GATCAGGTT‑GTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGG  >  NC_002947/1027858‑1028147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑