Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,028,172 G→A 6.9% Q190* (CAG→TAG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,028,1720GA 6.9% 165.5 / 2.4 58Q190* (CAG→TAG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

TCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGT  >  NC_002947/1028027‑1028319
                                                                                                                                                 |                                                                                                                                                   
tCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTc                                                                                                                                                  <  4:94446/149‑1 (MQ=255)
   ctCTTGACGTCCATGGGCTGCCAGTTTGCCATGGTGCGCGTGTAGGGGATCCTCGGGTCCTTTTTGTTTTTTGTTAAAAAGTACGGGCCGGTGGCGTTAGGGTTGTGGGTGATGGCGGCGGGCTTGGCGGTCTTTAGGAGCTGGTCGGc                                                                                                                                               <  2:107919/148‑1 (MQ=255)
     cTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCg                                                                                                                                              >  1:167680/1‑148 (MQ=255)
        cACGTCCTCGGGCTGCCAGTAGGCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGc                                                                                                                                               <  6:100673/144‑1 (MQ=255)
         aCGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTAtt                                                                                                                                          <  1:144738/148‑1 (MQ=255)
         aCGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTc                                                                                                                                         <  2:45544/149‑1 (MQ=255)
          cGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTcg                                                                                                                                        >  7:166221/1‑149 (MQ=255)
           gTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTcgg                                                                                                                                       >  8:137516/1‑149 (MQ=255)
            tCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTGCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTcggc                                                                                                                                      >  4:226919/1‑149 (MQ=255)
              cTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGGGGTGGTCGGCGTATTcggcg                                                                                                                                     >  2:151050/1‑148 (MQ=255)
              cTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTcggcg                                                                                                                                     <  6:183850/148‑1 (MQ=255)
              cTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTcggcg                                                                                                                                     <  2:167680/148‑1 (MQ=255)
                cGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCTGAc                                                                                                                                  >  2:204381/1‑149 (MQ=255)
                 gggCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGa                                                                                                                                   <  7:137516/147‑1 (MQ=255)
                 gggCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGAc                                                                                                                                  <  4:231811/148‑1 (MQ=255)
                 gggCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTGCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGa                                                                                                                                   <  3:226919/147‑1 (MQ=255)
                         agtagtCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAg                                                                                                                         <  8:37102/149‑1 (MQ=255)
                          gtagtCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTAGTCGGCGTATTCGGCGGACTGGACCGAgg                                                                                                                        >  3:275310/1‑149 (MQ=255)
                          gtagtCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTAGTCGGCGTATTCGGCGGACTGGACCGAg                                                                                                                         >  7:58347/1‑148 (MQ=255)
                            agtCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGc                                                                                                                       >  5:174126/1‑148 (MQ=255)
                              tCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCa                                                                                                                      >  3:319765/1‑147 (MQ=255)
                                        ccTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGcc                                                                                                          <  4:319765/149‑1 (MQ=255)
                                        ccTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTCGATCTAGGCAACGCTCATGGc                                                                                                           >  5:315505/1‑148 (MQ=255)
                                                         gcgTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAAcgc                                                                                         >  2:223897/1‑149 (MQ=255)
                                                         gcgTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAAcgc                                                                                         >  7:112724/1‑149 (MQ=255)
                                                              cTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGGGGCGt                                                                                    >  4:83565/1‑149 (MQ=255)
                                                               ttCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGt                                                                                    >  4:189928/1‑148 (MQ=255)
                                                               ttCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGt                                                                                    >  2:55995/1‑148 (MQ=255)
                                                                    gTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAAc                                                                                <  1:223897/147‑1 (MQ=255)
                                                                          gCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGtt                                                                          >  8:187541/1‑147 (MQ=255)
                                                                           cTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAg                                                                       <  1:204381/149‑1 (MQ=255)
                                                                            tGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAgg                                                                      >  1:80278/1‑149 (MQ=255)
                                                                                 acGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGaa                                                                 <  7:187541/149‑1 (MQ=255)
                                                                                   gAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACt                                                               >  2:219124/1‑149 (MQ=255)
                                                                                   gAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACt                                                               >  8:8742/1‑149 (MQ=255)
                                                                                          ccGGTGCCTATCGGCTGCTGGTTGATGGCGGCGGCCTTGCCGTTCTTTAGGAGCTGGGCGGCGTATTCGGCGGACTGGATCGAGGCACAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCgt                                                        <  5:247374/149‑1 (MQ=38)
                                                                                          ccGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCgt                                                        <  2:80278/149‑1 (MQ=255)
                                                                                                ccGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTc                                                  <  3:189928/149‑1 (MQ=255)
                                                                                                  gATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGt                                                >  4:92438/1‑149 (MQ=255)
                                                                                                       gCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGc                                           <  8:166221/149‑1 (MQ=255)
                                                                                                       gCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGc                                           <  8:112724/149‑1 (MQ=255)
                                                                                                       gCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGc                                           >  7:220956/1‑149 (MQ=255)
                                                                                                       gCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTAGTCGGCGTATTCGGCGGACTGGACCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGc                                           <  8:58347/149‑1 (MQ=255)
                                                                                                       gCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTAGTCGGCGTATTCGGCGGACTGGACCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGc                                           <  4:275310/149‑1 (MQ=255)
                                                                                                              gTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTcc                                     <  1:219124/148‑1 (MQ=255)
                                                                                                              gTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCa                                    >  4:36237/1‑149 (MQ=255)
                                                                                                                     tCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCGAGCTTCTCCACCTTgg                              <  6:174126/148‑1 (MQ=255)
                                                                                                                        gcggaCTTGCCTTTCTTTAGGAGCTGGTCGGAGTATTCGGCGGACTGGATCTAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGTTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAAt                          <  7:90985/149‑1 (MQ=38)
                                                                                                                            ccTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGTTCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTc                      <  3:670/149‑1 (MQ=255)
                                                                                                                            ccTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGTTCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTc                      >  4:670/1‑149 (MQ=255)
                                                                                                                            ccTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTc                      <  3:92438/149‑1 (MQ=255)
                                                                                                                              ttGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCtt                    >  2:179304/1‑149 (MQ=255)
                                                                                                                                tccGTTCTTTAGGAGGTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCtt                    <  3:153992/146‑1 (MQ=255)
                                                                                                                                gCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAAcgcg                                                                                        >  4:205141/1‑79 (MQ=255)
                                                                                                                                gCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAAcgcg                                                                                        <  3:205141/79‑1 (MQ=255)
                                                                                                                                gCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCtt                    <  3:83565/147‑1 (MQ=255)
                                                                                                                                gCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCtt                    <  7:8742/147‑1 (MQ=255)
                                                                                                                                                tGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGt  >  2:83876/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGCGGCCTTGCCGTTCTTTAGGAGCTGGTCGGCGTATTCGGCGGACTGGATCGAGGCAAAGCTCATGGCCAGGTTCTGGATGAACGCGGCGTCAACCTCGTTGAGGGTGAACTTCACCGTGTGCTCGTCAAGCTTCTCCACCTTGGCAATGTTCTTGTCCATGCCCATGTCGGT  >  NC_002947/1028027‑1028319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑