Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,090,032 T→G 7.0% L298R (CTG→CGG)  fumC‑I → class 2 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,090,0320TG 7.0% 207.2 / 5.0 68L298R (CTG→CGG) fumC‑Iclass 2 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base G (3/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGC  >  NC_002947/1089885‑1090173
                                                                                                                                                    |                                                                                                                                              
ccAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTg                                                                                                                                               <  3:107669/149‑1 (MQ=255)
 cAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTTATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTgg                                                                                                                                              <  8:120857/149‑1 (MQ=255)
    ggTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCACACGATCTGCGCTGGATGAACTCCGGCCCGCTGGc                                                                                                                                             <  1:45155/148‑1 (MQ=255)
    ggTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACACCGGCCCGCTGGc                                                                                                                                             >  2:45155/1‑147 (MQ=255)
        gAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCAGCGGGCCggag                                                                                                                                        <  1:330148/148‑3 (MQ=255)
          gTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCGGGCCGGTCTgg                                                                                                                                     >  6:180273/1‑149 (MQ=255)
           ttCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTNGATGAACTCCGGCCCGCTGGCCGGTCTgg‑g                                                                                                                                   <  1:29649/149‑1 (MQ=255)
             cACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGTGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑Gc                                                                                                                                  >  3:215918/1‑148 (MQ=255)
              aCGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCg                                                                                                                                 <  7:148597/148‑1 (MQ=255)
                gCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGaaa                                                                                                                              >  8:177141/1‑149 (MQ=255)
                   cGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATc                                                                                                                            >  1:1538/1‑148 (MQ=255)
                   cGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATc                                                                                                                            >  1:42314/1‑148 (MQ=255)
                    ggCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGa                                                                                                                          <  7:17844/149‑1 (MQ=255)
                     gCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAg                                                                                                                         >  7:33626/1‑149 (MQ=255)
                            ccTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCAGCGGGCCggag                                                                                                                                        <  2:251135/128‑3 (MQ=255)
                            ccTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCAGCGGGCCggag                                                                                                                                        >  1:251135/1‑126 (MQ=255)
                                ttCGCACTGATCGGCTCGCAGGACACTGCCGGGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGctc                                                                                                               <  2:158352/148‑1 (MQ=255)
                                  cGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTg                                                                                                             >  6:54024/1‑148 (MQ=255)
                                   gCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAg                                                                                                                  <  1:22196/142‑1 (MQ=255)
                                   gCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAg                                                                                                                  >  2:22196/1‑142 (MQ=255)
                                         aTCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCggg                                                                                                     <  6:60443/149‑1 (MQ=255)
                                         aTCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCgg                                                                                                      <  5:54024/148‑1 (MQ=255)
                                              cTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGTGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCtt                                                                                                <  4:215918/149‑1 (MQ=255)
                                                 gCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGGAGCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCtt                                                                                                <  1:54029/147‑1 (MQ=255)
                                                  cAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGAt                                                                                            >  6:337148/1‑149 (MQ=255)
                                                         cTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCa                                                                                     <  3:70973/149‑1 (MQ=255)
                                                            ccGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCt                                                                                                                       <  1:26210/112‑1 (MQ=255)
                                                            ccGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCt                                                                                                                       >  2:26210/1‑112 (MQ=255)
                                                             cGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGc                                                                                  >  3:85346/1‑148 (MQ=255)
                                                                ggCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAgg                                                                              <  7:49864/149‑1 (MQ=255)
                                                                 gCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGt                                                                             >  6:21948/1‑149 (MQ=255)
                                                                  cTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGACA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGt                                                                             >  5:318388/1‑148 (MQ=255)
                                                                      gTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGCGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAAcc                                                                        >  8:274260/1‑149 (MQ=255)
                                                                       tCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGc                                                                                                                     <  4:305968/103‑1 (MQ=255)
                                                                       tCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGAt                                                                                            <  7:299702/128‑1 (MQ=255)
                                                                       tCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGAt                                                                                            >  8:299702/1‑128 (MQ=255)
                                                                         tGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGcc                                                                                      <  4:175625/132‑1 (MQ=255)
                                                                         tGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGcc                                                                                      >  3:175625/1‑132 (MQ=255)
                                                                           gCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAATGTCAACCCg                                                                      <  6:318388/146‑1 (MQ=255)
                                                                           gCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGt                                                                    <  2:1538/148‑1 (MQ=255)
                                                                           gCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGt                                                                    <  7:11213/148‑1 (MQ=255)
                                                                           gCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGt                                                                    >  8:267630/1‑148 (MQ=255)
                                                                           gCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTg                                                                   <  5:134970/149‑1 (MQ=255)
                                                                                 tCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCg                                                             >  4:41432/1‑149 (MQ=255)
                                                                                   aaGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGa                                                           <  5:21948/149‑1 (MQ=255)
                                                                                    aGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAg                                                          <  8:33626/149‑1 (MQ=255)
                                                                                      cccaccGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGc                                                        <  5:337148/148‑1 (MQ=255)
                                                                                      accaccGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGc                                                        >  8:56684/1‑149 (MQ=255)
                                                                                        caccGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCTCCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGAAGCCGGGCTCTTCGATCATCCCAGGAAAGGTCAACCCGGTGATCCCGGAGGACa                                                      >  2:319559/1‑149 (MQ=255)
                                                                                             cTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGa                                                           <  8:39556/139‑1 (MQ=255)
                                                                                             cTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGa                                                           >  7:39556/1‑139 (MQ=255)
                                                                                                    gTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTcg                                          >  7:270487/1‑149 (MQ=255)
                                                                                                     ttGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTcgc                                         <  8:205377/149‑1 (MQ=255)
                                                                                                           aaaaTCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGT‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGcc                                                 <  8:147770/135‑1 (MQ=255)
                                                                                                           aaaaTCGCCA‑ACGATCTGCGCTGGATGAACTCAGGCCCGCTCGCCGGACTGG‑GCGAAATCGAGCTACAAGCTCTGAAGCCGGGCTCTTCGATCAAGCCAGGCAAGGTCAACACGGTGATCCCGGAGGCCACTccc                                                 >  7:147770/1‑135 (MQ=255)
                                                                                                                 gCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGAt                             >  8:116706/1‑149 (MQ=255)
                                                                                                                  ccA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGc                                                        >  4:191724/1‑121 (MQ=255)
                                                                                                                  ccA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGc                                                        <  3:191724/121‑1 (MQ=255)
                                                                                                                   cA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTg                           <  7:177141/149‑1 (MQ=255)
                                                                                                                    a‑aCGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTgg                          <  5:180273/149‑1 (MQ=255)
                                                                                                                                     tGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATc          >  5:17059/1‑149 (MQ=255)
                                                                                                                                      ccagaccGGCCAGCGGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCg         >  3:77254/6‑149 (MQ=255)
                                                                                                                                         cTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCg      >  5:2739/1‑149 (MQ=255)
                                                                                                                                          tCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAg                                 >  4:79816/1‑121 (MQ=255)
                                                                                                                                          tCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAg                                 <  3:79816/121‑1 (MQ=255)
                                                                                                                                          tCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGt     >  4:241624/1‑149 (MQ=255)
                                                                                                                                          tCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCAACGCCGt     >  8:202158/1‑149 (MQ=255)
                                                                                                                                             ggCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGc  <  7:267630/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTCAAGACCACCGCTGTGGCGTTGATGAAAATCGCCA‑ACGATCTGCGCTGGATGAACTCCGGCCCGCTGGCCGGTCTGG‑GCGAAATCGAGCTGCAAGCTCTGCAGCCGGGCTCTTCGATCATGCCAGGCAAGGTCAACCCGGTGATCCCGGAGGCCACTGCCATGGTCGCCGCCCAAGTGATTGGCAACGACGCGACCATCGCCGTCGC  >  NC_002947/1089885‑1090173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑