Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,169,305 T→C 8.9% I369V (ATC→GTC)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,169,3050TC 8.9% 153.9 / 5.3 56I369V (ATC→GTC) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/28);  new base C (2/3);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

CGGTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACC  >  NC_002947/1169157‑1169450
                                                                                                                                                    |                                                                                                                                                 
cGGTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGAt                                                                                                                                                   <  1:74335/149‑1 (MQ=255)
 ggTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATg                                                                                                                                                  >  1:105828/1‑149 (MQ=255)
      gTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGc                                                                                                                                             <  5:309255/149‑1 (MQ=255)
           aCCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        >  6:26510/1‑149 (MQ=255)
           aCCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACTTCGGCCgcag                                                                                                                                        <  2:112630/149‑4 (MQ=255)
             ccTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       >  2:283033/1‑148 (MQ=255)
              cTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     <  7:39266/149‑7 (MQ=255)
                 caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   >  4:143041/1‑148 (MQ=255)
                 caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   <  3:220307/148‑1 (MQ=255)
                 caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   <  3:143041/148‑1 (MQ=255)
                    caccTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCa                                                                                                                               <  4:43999/149‑1 (MQ=255)
                     atcTGGGTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGGCATTTTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGAGGATTACCGCTTCGTTGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAg                                                                                                                              <  8:98532/147‑1 (MQ=255)
                            tGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAgg                                                                                                                        >  7:220857/1‑148 (MQ=255)
                                ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        >  7:125270/1‑128 (MQ=255)
                                ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        <  8:125270/128‑1 (MQ=255)
                                ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCt                                                                                                                   >  5:265149/1‑149 (MQ=255)
                                 tCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCggg                                                                                                                                         >  6:276329/1‑126 (MQ=255)
                                 tCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCggg                                                                                                                                         <  5:276329/126‑1 (MQ=255)
                                   gATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCg                                                                                                                                           <  1:229414/122‑1 (MQ=255)
                                   gATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCg                                                                                                                                           >  2:229414/1‑122 (MQ=255)
                                    aTCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCca                                                                                                               >  1:290866/1‑149 (MQ=255)
                                       tGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     <  5:324893/124‑7 (MQ=255)
                                       tGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     >  6:324893/1‑118 (MQ=255)
                                         cATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCca                                                                                                               <  3:116086/144‑1 (MQ=255)
                                         cATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAg                                                                                                            <  6:117589/147‑1 (MQ=255)
                                          aTGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTc                                                                                                         >  2:264444/1‑149 (MQ=255)
                                                         cTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCtt                                                                                          <  5:26510/149‑1 (MQ=255)
                                                          tGATGCCATATTCCTGCTCGAGCAGGTAGATGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTg                                                                                         >  5:62255/1‑149 (MQ=255)
                                                           gATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATT‑CCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGc                                                                                       >  4:183278/1‑149 (MQ=255)
                                                            aTGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGc                                                                                       <  4:282598/149‑1 (MQ=255)
                                                             tGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       <  5:175516/116‑1 (MQ=255)
                                                             tGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       >  6:175516/1‑116 (MQ=255)
                                                                cATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGc                                                                                                                                >  4:54626/1‑104 (MQ=255)
                                                                cATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGc                                                                                                                                <  3:54626/104‑1 (MQ=255)
                                                                 atatTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGc                                                                                  <  3:40004/149‑1 (MQ=255)
                                                                   atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       >  1:251786/1‑94 (MQ=255)
                                                                   atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       <  2:251786/94‑1 (MQ=255)
                                                                   atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATc                                                                                                   >  5:10577/1‑130 (MQ=255)
                                                                   atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATc                                                                                                   <  6:10577/130‑1 (MQ=255)
                                                                             gAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATg                                                                       <  1:283033/148‑1 (MQ=255)
                                                                                    taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGc                                                                            >  3:164579/1‑136 (MQ=255)
                                                                                    taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGc                                                                            <  4:164579/136‑1 (MQ=255)
                                                                                    taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTg                                                               <  2:55760/149‑1 (MQ=255)
                                                                                      ggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  7:141876/149‑1 (MQ=255)
                                                                                       gtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  8:220857/148‑1 (MQ=255)
                                                                                          aTACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       <  2:189961/87‑1 (MQ=255)
                                                                                          aTACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       >  1:189961/1‑87 (MQ=255)
                                                                                              cGCCTTTGCCCGACTGGCTGTTGACGCGGATT‑CCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCgg                                                    <  3:183278/149‑1 (MQ=255)
                                                                                               gCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             >  3:69119/1‑140 (MQ=255)
                                                                                               gCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  4:69119/140‑1 (MQ=255)
                                                                                                ccTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGga                                                   <  6:62255/149‑1 (MQ=255)
                                                                                                                     ccgcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAAc                                               <  4:46010/131‑1 (MQ=255)
                                                                                                                          gATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCca                         <  1:264444/149‑1 (MQ=255)
                                                                                                                               ccGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACagag                    >  3:247880/1‑149 (MQ=255)
                                                                                                                                           tGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGa        >  8:60100/1‑149 (MQ=255)
                                                                                                                                            ccGGCCGACATCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTT‑GCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATg      >  5:8642/2‑149 (MQ=255)
                                                                                                                                                 cGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTAcc  >  2:139698/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACC  >  NC_002947/1169157‑1169450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑