Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,169,306 G→A 7.4% D368D (GAC→GAT)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,169,3060GA 7.4% 159.8 / 2.1 55D368D (GAC→GAT) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (22/28);  new base A (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

GGTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACC  >  NC_002947/1169158‑1169450
                                                                                                                                                    |                                                                                                                                                
ggTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATg                                                                                                                                                  >  1:105828/1‑149 (MQ=255)
     gTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGc                                                                                                                                             <  5:309255/149‑1 (MQ=255)
          aCCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        >  6:26510/1‑149 (MQ=255)
          aCCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACTTCGGCCgcag                                                                                                                                        <  2:112630/149‑4 (MQ=255)
            ccTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       >  2:283033/1‑148 (MQ=255)
             cTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     <  7:39266/149‑7 (MQ=255)
                caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   >  4:143041/1‑148 (MQ=255)
                caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   <  3:220307/148‑1 (MQ=255)
                caccacCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATc                                                                                                                                   <  3:143041/148‑1 (MQ=255)
                   caccTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCa                                                                                                                               <  4:43999/149‑1 (MQ=255)
                    atcTGGGTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGGCATTTTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGAGGATTACCGCTTCGTTGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAg                                                                                                                              <  8:98532/147‑1 (MQ=255)
                           tGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAgg                                                                                                                        >  7:220857/1‑148 (MQ=255)
                               ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        <  8:125270/128‑1 (MQ=255)
                               ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGt                                                                                                                                        >  7:125270/1‑128 (MQ=255)
                               ttCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCt                                                                                                                   >  5:265149/1‑149 (MQ=255)
                                tCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCggg                                                                                                                                         >  6:276329/1‑126 (MQ=255)
                                tCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCggg                                                                                                                                         <  5:276329/126‑1 (MQ=255)
                                  gATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCg                                                                                                                                           >  2:229414/1‑122 (MQ=255)
                                  gATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCg                                                                                                                                           <  1:229414/122‑1 (MQ=255)
                                   aTCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCca                                                                                                               >  1:290866/1‑149 (MQ=255)
                                      tGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     >  6:324893/1‑118 (MQ=255)
                                      tGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGACATCGGCCgcagcta                                                                                                                                     <  5:324893/124‑7 (MQ=255)
                                        cATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCca                                                                                                               <  3:116086/144‑1 (MQ=255)
                                        cATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAg                                                                                                            <  6:117589/147‑1 (MQ=255)
                                         aTGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTc                                                                                                         >  2:264444/1‑149 (MQ=255)
                                                        cTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCtt                                                                                          <  5:26510/149‑1 (MQ=255)
                                                         tGATGCCATATTCCTGCTCGAGCAGGTAGATGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTg                                                                                         >  5:62255/1‑149 (MQ=255)
                                                          gATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATT‑CCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGc                                                                                       >  4:183278/1‑149 (MQ=255)
                                                           aTGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGc                                                                                       <  4:282598/149‑1 (MQ=255)
                                                            tGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       >  6:175516/1‑116 (MQ=255)
                                                            tGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       <  5:175516/116‑1 (MQ=255)
                                                               cATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGc                                                                                                                                >  4:54626/1‑104 (MQ=255)
                                                               cATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGc                                                                                                                                <  3:54626/104‑1 (MQ=255)
                                                                atatTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGc                                                                                  <  3:40004/149‑1 (MQ=255)
                                                                  atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       >  1:251786/1‑94 (MQ=255)
                                                                  atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTc                                                                                                                                       <  2:251786/94‑1 (MQ=255)
                                                                  atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATc                                                                                                   >  5:10577/1‑130 (MQ=255)
                                                                  atTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATc                                                                                                   <  6:10577/130‑1 (MQ=255)
                                                                            gAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATg                                                                       <  1:283033/148‑1 (MQ=255)
                                                                                   taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGc                                                                            <  4:164579/136‑1 (MQ=255)
                                                                                   taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGc                                                                            >  3:164579/1‑136 (MQ=255)
                                                                                   taggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTg                                                               <  2:55760/149‑1 (MQ=255)
                                                                                     ggtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  7:141876/149‑1 (MQ=255)
                                                                                      gtGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  8:220857/148‑1 (MQ=255)
                                                                                         aTACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       >  1:189961/1‑87 (MQ=255)
                                                                                         aTACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGc                                                                                                                       <  2:189961/87‑1 (MQ=255)
                                                                                             cGCCTTTGCCCGACTGGCTGTTGACGCGGATT‑CCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCgg                                                    <  3:183278/149‑1 (MQ=255)
                                                                                              gCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             >  3:69119/1‑140 (MQ=255)
                                                                                              gCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGt                                                             <  4:69119/140‑1 (MQ=255)
                                                                                               ccTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGga                                                   <  6:62255/149‑1 (MQ=255)
                                                                                                                    ccgcgGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAAc                                               <  4:46010/131‑1 (MQ=255)
                                                                                                                         gATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCca                         <  1:264444/149‑1 (MQ=255)
                                                                                                                              ccGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACagag                    >  3:247880/1‑149 (MQ=255)
                                                                                                                                          tGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGa        >  8:60100/1‑149 (MQ=255)
                                                                                                                                           ccGGCCGACATCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTT‑GCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATg      >  5:8642/2‑149 (MQ=255)
                                                                                                                                                cGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTAcc  >  2:139698/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGTCAGTCTCACCCTGCACCACCTGGCTGAATTCGATCTGCATGCGACGCGGCAGGCTGATGCCATATTCCTGCTCGAGCAGGTAGGTGATACCGCCTTTGCCCGACTGGCTGTTGACGCGGATTACCGCTTCGTAGCTGCGGCCGATGTCGGCCGGGTCGATCGGCAGGTAAGGCACCTCCCACAGTTCCCCATCCTGCTGCTTGGCGAAGCCCTTGCGGATGGCGTCCTGGTGCGAGCCGGAGAACGCGGTATGGACCAGATCCCCCACATAGGGGTGACGAGGATGTACC  >  NC_002947/1169158‑1169450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑