Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,172,293 C→T 100% A254T (GCT→ACT)  xseA ← exodeoxyribonuclease 7 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,172,2930CT100.0% 138.0 / NA 42A254T (GCT→ACT) xseAexodeoxyribonuclease 7 large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/22);  total (20/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCCAGGCGCAGGCGGTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGCCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTCCAGCGAACCCCCACCCCGCGCCAGGAT  >  NC_002947/1172148‑1172434
                                                                                                                                                 |                                                                                                                                             
tCCAGGCGCAGGCGGTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCAc                                                                                                                                            >  6:113925/1‑149 (MQ=255)
tCCAGGCGCAGGCGGTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCAc                                                                                                                                            >  2:110016/1‑149 (MQ=255)
         aggcgGTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCt                                                                                                                                   >  7:33151/1‑149 (MQ=255)
             ggTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATc                                                                                                                               <  3:321187/149‑1 (MQ=255)
                   gggTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTAGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACg                                                                                                                         >  6:178210/1‑149 (MQ=255)
                         ggCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGtt                                                                                                                   >  1:266853/1‑149 (MQ=255)
                         ggCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGtt                                                                                                                   <  2:266853/149‑1 (MQ=255)
                           cGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTc                                                                                                                 <  8:141665/149‑1 (MQ=255)
                            ggTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCa                                                                                                                >  4:22477/1‑149 (MQ=255)
                                  gCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGa                                                                                                                             >  1:243212/1‑130 (MQ=255)
                                  gCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGa                                                                                                                             <  2:243212/130‑1 (MQ=255)
                                            cagcagGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGa                                                                                                <  1:110016/149‑1 (MQ=255)
                                            cagcagGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGa                                                                                                <  7:132948/149‑1 (MQ=255)
                                            cagcagGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGa                                                                                                <  7:279209/149‑1 (MQ=255)
                                                    gcCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGa                                                                                                <  7:86857/141‑1 (MQ=255)
                                                    gcCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGa                                                                                                >  8:86857/1‑141 (MQ=255)
                                                      cGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGt                                                                                      >  2:39636/1‑149 (MQ=255)
                                                      cGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGt                                                                                      <  6:110547/149‑1 (MQ=255)
                                                           cAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACg                                                                                  <  3:22477/148‑1 (MQ=255)
                                                             ggCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAg                                                                               >  5:202361/1‑149 (MQ=255)
                                                                             ccGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGTGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCAcgc                                                                <  6:47679/148‑1 (MQ=255)
                                                                               gAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCAcgc                                                                >  7:61067/1‑146 (MQ=255)
                                                                               gAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCAcgc                                                                <  8:61067/146‑1 (MQ=255)
                                                                               gAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGcc                                                             >  2:162007/1‑149 (MQ=255)
                                                                                  ggCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGc                                                                                 <  3:230387/126‑1 (MQ=255)
                                                                                  ggCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGc                                                                                 >  4:230387/1‑126 (MQ=255)
                                                                                  ggCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACg                                                          <  1:162007/149‑1 (MQ=255)
                                                                                         gttgtCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCt                                                   >  8:129309/1‑149 (MQ=255)
                                                                                          ttgtCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGgcgc                                                                                                   >  4:194247/1‑100 (MQ=255)
                                                                                          ttgtCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGgcgc                                                                                                   <  3:194247/100‑1 (MQ=255)
                                                                                            gtCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTCGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCg                                                 <  5:178210/148‑1 (MQ=255)
                                                                                                ggggCCAGCAGCTCGGCTGCCGCAGACGGGGTGGGCTCGCGCACGTGAGTGACGAAGTCGCTGATCGACACGTCGGTGTCATGGCCCACGGCGCTGACAATCGTCGTGACGCAGTCGGCCAAGGCACTCTCCACGGCTTCCTCGTTGaa                                            <  8:96533/149‑1 (MQ=255)
                                                                                                      agcagcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTc                                                  >  2:37684/1‑137 (MQ=255)
                                                                                                      agcagcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTc                                                  <  1:37684/137‑1 (MQ=255)
                                                                                                       gcagcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAAcaccac                                     <  6:202361/149‑1 (MQ=255)
                                                                                                        cagcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAAcacca                                      >  7:196575/1‑147 (MQ=255)
                                                                                                        cagcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACcaca                                    <  7:66768/149‑1 (MQ=255)
                                                                                                         agcTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAg                                   <  8:196575/149‑1 (MQ=255)
                                                                                                           cTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAgg                                  >  5:73392/1‑148 (MQ=255)
                                                                                                               gcggcCGCAGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTc                             >  1:302803/1‑149 (MQ=255)
                                                                                                                       aGACGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTCCAGCGAAc                     >  5:330979/1‑149 (MQ=255)
                                                                                                                          cGGGGTGGGCGCGCGCACGTCAGTCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTCCAGCGAAcccc                  <  8:33151/149‑1 (MQ=255)
                                                                                                                                            gtcagtcaCGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTCCAGCGAACCCCCACCCCGCGCCAGGAt  >  5:131728/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCCAGGCGCAGGCGGTCGTGGGTCAGGCGGTTCTGCATGCGCAGCAGCAGGCGCCGTTGCAGGCCATCCAGGCGTTGCCGAAGGCCGCTGTTGTCAGGGGCCAGCAGCTCGGCGGCCGCAGACGGGGTGGGCGCGCGCACGTCAGCCACGAAGTCGCTGATCGACACGTCGGTTTCATGGCCCACGGCGCTGACAATCGGCGTGACGCAGGCGGCCACGGCACGCGCCACGGCTTCCTCGTTGAAACACCACAGGTCTTCCAGCGAACCCCCACCCCGCGCCAGGAT  >  NC_002947/1172148‑1172434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑