| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,184,588 | G→C | 22.8% | intergenic (‑204/‑208) | mltF ← / → purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,184,588 | 0 | G | C | 22.8% | 37.9 / 9.1 | 22 | intergenic (‑204/‑208) | mltF/purL | membrane‑bound lytic murein transglycosylase F/phosphoribosylformylglycinamidine synthase |
| Reads supporting (aligned to +/- strand): ref base G (10/7); new base C (3/2); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
GTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGC > NC_002947/1184445‑1184734 | gTAGCGGGTTGGGTGGTGGGGGGAGGGGTGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCgcgggc < 6:320206/146‑1 (MQ=255) gggTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCg < 1:130299/149‑1 (MQ=255) ttGTGTGGTTGGGGGAGGGGGGATTGCTTCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGAtc < 8:178109/149‑1 (MQ=255) gggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCCCGCGCGGCGCTCGAt < 1:117948/131‑1 (MQ=255) gggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCCCGCGCGGCGCTCGAt > 2:117948/1‑131 (MQ=255) cATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcg > 8:277942/1‑132 (MQ=255) cATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcg < 7:277942/132‑1 (MQ=255) aTCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGAtctc > 3:60522/1‑124 (MQ=255) aTCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGAtctc < 4:60522/124‑1 (MQ=255) cGCCTTAAGATATTCAGCGCCTATGAGGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCCCAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcccc > 1:13855/1‑138 (MQ=255) cGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcccc < 2:13855/137‑1 (MQ=255) gCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTcc > 8:172208/1‑149 (MQ=255) ccTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑gcggcg > 2:59241/1‑93 (MQ=255) ttAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCAAAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCACGTCTCAAGCCGGACACCTCAGAAAACTGACACCGAAGTCCGGTTccgcc > 1:298676/1‑149 (MQ=255) ga‑gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTag > 7:72232/1‑149 (MQ=255) a‑gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTaga < 7:172208/149‑1 (MQ=255) gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcccc > 3:113117/1‑111 (MQ=255) gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcccc < 4:113117/111‑1 (MQ=255) gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGGCGCTGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTagag > 6:308820/1‑149 (MQ=255) cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAg > 5:279481/1‑149 (MQ=255) gcgcCGCCCGCGCGGCGCTCGATCTCACACGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTg > 4:232932/1‑149 (MQ=255) gccgccCGCTCGGCGCTCGATCTCATAGGCGCTGAATATCTTAAGGCGGACACCTGGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc < 7:259997/149‑1 (MQ=18) cgcgcgGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCg > 5:228988/1‑148 (MQ=255) gggcggCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGc > 1:148076/1‑148 (MQ=255) | GTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGA‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCT‑GCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGC > NC_002947/1184445‑1184734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |