Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,236,968 G→A 9.4% intergenic (+7/+31) ybaK → / ← PP_1078 Cys‑tRNAPro deacylase/ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,236,9680GA 9.4% 146.0 / 4.5 53intergenic (+7/+31)ybaK/PP_1078Cys‑tRNAPro deacylase/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base A (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

CCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCCACATTC  >  NC_002947/1236825‑1237116
                                                                                                                                               |                                                                                                                                                    
ccTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGgcc                                                                                                                                                 <  3:155638/149‑1 (MQ=255)
 cTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGgccg                                                                                                                                                >  5:319582/1‑149 (MQ=255)
          tgaCTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCAGAGTACACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCgg                                                                                                                                        <  1:99531/148‑1 (MQ=255)
                ggCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTg                                                                                                                                 <  5:316826/149‑1 (MQ=255)
                ggCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGAGGGCCGCTTGGCGGCCccct                                                                                                                                  <  6:113534/148‑1 (MQ=255)
                    cAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTc                                                                                                                             >  7:120011/1‑149 (MQ=255)
                    cAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGAGGGCCGCTTGGCGGCCCCCTgttct                                                                                                                             <  5:159598/149‑5 (MQ=255)
                     aGAATTTTGCAAATATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGGTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCt                                                                                                                            <  1:36234/149‑1 (MQ=255)
                        aTTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGaa                                                                                                                         <  5:80559/149‑1 (MQ=255)
                        aTTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGaa                                                                                                                         <  5:85316/149‑1 (MQ=255)
                              cAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGaagtcaa                                                                                                                    <  7:15638/148‑1 (MQ=255)
                               aaCTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGaagtcaag                                                                                                                   >  4:167435/1‑148 (MQ=255)
                               aaCTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGAGGGCCGCTTGGCGGCCCCCTgttc                                                                                                                              <  5:264056/137‑4 (MQ=255)
                               aaCTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGAGGGCCGCTTGGCGGCCCCCTgttc                                                                                                                              >  6:264056/1‑134 (MQ=255)
                                              cgcCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGc                                                                                                                                          <  6:37108/110‑1 (MQ=255)
                                              cgcCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGc                                                                                                                                          >  5:37108/1‑110 (MQ=255)
                                                          tGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCAtt                                                                                       >  4:27204/1‑149 (MQ=255)
                                                            gCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATtct                                                                                     >  3:63320/1‑149 (MQ=255)
                                                              cTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAgggg                                                                                                                                                    <  4:299338/84‑1 (MQ=255)
                                                              cTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAgggg                                                                                                                                                    >  3:299338/1‑84 (MQ=255)
                                                              cTGGAACTGGAGTTGGCCGCAGCGGTGCTGGAGGAGTATACGCACGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAAGGAGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTcc                                                                                   >  7:206505/1‑149 (MQ=255)
                                                                 gAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTg                                                                                 <  8:120011/148‑1 (MQ=255)
                                                                            ggCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACtg                                                                     >  1:322869/1‑149 (MQ=255)
                                                                            ggCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACtg                                                                     >  3:150378/1‑149 (MQ=255)
                                                                                      gTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACg                                                           >  4:249662/1‑149 (MQ=255)
                                                                                          tGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAc                                                       >  4:132894/1‑149 (MQ=255)
                                                                                           ggCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAcc                                                      <  8:130622/149‑1 (MQ=255)
                                                                                            gCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAccc                                                     >  3:61140/1‑149 (MQ=255)
                                                                                            gCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAccc                                                     >  3:31722/1‑149 (MQ=255)
                                                                                            gCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAcc                                                      <  3:167435/148‑1 (MQ=255)
                                                                                                  tataCGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTAcc                                               >  1:299450/1‑149 (MQ=255)
                                                                                                    taCGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCgg                                             <  2:299450/149‑1 (MQ=255)
                                                                                                        cAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGCACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGaa                                          >  3:99889/1‑148 (MQ=255)
                                                                                                            gCACGTTTGCCGGGTTTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCa                                     <  8:206505/149‑1 (MQ=255)
                                                                                                            gCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCa                                     >  3:94800/1‑149 (MQ=255)
                                                                                                            gCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCAGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCa                                     <  6:319582/149‑1 (MQ=255)
                                                                                                              aCGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCggg                                            >  7:176189/1‑140 (MQ=255)
                                                                                                              aCGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCggg                                            <  8:176189/140‑1 (MQ=255)
                                                                                                               cGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGa                                  <  4:63320/149‑1 (MQ=255)
                                                                                                                gTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAg                                 <  3:27204/149‑1 (MQ=255)
                                                                                                                 tttGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAgg                                <  4:61140/149‑1 (MQ=255)
                                                                                                                    gCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCgg                                             <  2:232509/133‑1 (MQ=255)
                                                                                                                    gCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCgg                                             >  1:232509/1‑133 (MQ=255)
                                                                                                                      cGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCAGGAATAGCAAGAGGTGATc                           >  8:248732/1‑149 (MQ=255)
                                                                                                                             ggTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCtt                     <  4:150378/148‑1 (MQ=255)
                                                                                                                             ggTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCtt                     <  2:322869/148‑1 (MQ=255)
                                                                                                                               tCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCg                                              <  4:79267/121‑1 (MQ=255)
                                                                                                                               tCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCg                                              >  3:79267/1‑121 (MQ=255)
                                                                                                                                   ggCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCaa                                                             >  4:118980/1‑102 (MQ=255)
                                                                                                                                   ggCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCaa                                                             <  3:118980/102‑1 (MQ=255)
                                                                                                                                      tGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAccc                                                     <  4:64984/107‑1 (MQ=255)
                                                                                                                                      tGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTAccc                                                     >  3:64984/1‑107 (MQ=255)
                                                                                                                                          aaCAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCca       >  1:243643/1‑149 (MQ=255)
                                                                                                                                            cAGAGGGCCGCTTGGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCcaca     >  1:310955/1‑149 (MQ=255)
                                                                                                                                             aGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAAATGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCCACAt    >  6:178573/1‑149 (MQ=255)
                                                                                                                                               ggggCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGACGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCCACATtc  >  8:291249/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCTTTATTGATGACTCGGCGCAGAATTTTGCAACTATCCATGTCAGCGCCGGCAGGCGTGGCCTGGAAGTGGAGTTGGCCGCAGCGGTGCTGGCGGAGTATACGCAGGGCACGTTTGCCGGGATTGGTCGAGGCTGAGAACAGGGGGCCGCCAAGCGGCCCTCTGGTTCTGAAGTCAAGTCGCCGAACTGTATCGACGCACGCCATTCTCCTGGCGTGGCAACTGTGCCGCAACGCTACCCGGTACCGGGAATAGCACGAGGTGATCGGCCTTCACTGCAATCTCCACATTC  >  NC_002947/1236825‑1237116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑