Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,248,213 C→A 8.1% intergenic (+26/+42) argG → / ← PP_1089 argininosuccinate synthase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,248,2130CA 8.1% 188.7 / 2.3 60intergenic (+26/+42)argG/PP_1089argininosuccinate synthase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base A (3/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAACATCGCCACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTGGCTCGGGCG  >  NC_002947/1248065‑1248361
                                                                                                                                                    |                                                                                                                                                    
gCCAACATCGCCACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGc                                                                                                                                                      >  6:329505/1‑149 (MQ=255)
  cAACATCGCCACCTTTGAAGAAGATGGAGGAGCCTACAACCAGGCAGATGCAGCTGGCTTCATCAAGCTCAATGCACTGCGTATGAGCATTGCCGCCAACAAGGGCCGTTCGCAGCTCTGATTGATGGCGTGTGCGAAAACCCCGGCTc                                                                                                                                                    >  1:81676/1‑149 (MQ=255)
   aaCATCGCCACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGTGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCg                                                                                                                                                   >  1:179558/1‑149 (MQ=255)
      aTCGCCACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTc                                                                                                                                                >  6:126781/1‑149 (MQ=255)
          ccACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCggg                                                                                                                                            <  5:86581/149‑1 (MQ=255)
          ccACCTTTGAAGAAGATGGTGGGGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCggg                                                                                                                                            <  4:13970/149‑1 (MQ=255)
           cACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTc                                                                                                                                                <  6:19239/144‑1 (MQ=255)
           cACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTc                                                                                                                                                >  5:19239/1‑144 (MQ=255)
              cTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGACCGAGCCGGGGttt                                                                                                                                        <  7:168312/149‑1 (MQ=255)
               tttGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGtttt                                                                                                                                       <  8:111288/149‑1 (MQ=255)
                 tGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGttttt                                                                                                                                      <  5:329505/148‑1 (MQ=255)
                    agaagaTGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTAt                                                                                                                                  <  1:118050/149‑1 (MQ=255)
                    agaagaTGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTAt                                                                                                                                  <  7:82179/149‑1 (MQ=255)
                     gaagaTGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATg                                                                                                                                 <  3:280620/149‑1 (MQ=255)
                     gaagaTGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATg                                                                                                                                 >  4:257913/1‑149 (MQ=255)
                          tggtggTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTATTTTTATGCCcgc                                                                                                                            >  1:275621/1‑149 (MQ=255)
                                tgCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAg                                                                                                                      <  2:175698/149‑1 (MQ=255)
                                  ccTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGc                                                                                                                    <  3:57055/149‑1 (MQ=255)
                                          cAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTACGCTGCTCTGATTGCTGGCGTGAGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGtt                                                                                                            >  2:263559/1‑149 (MQ=255)
                                                gATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACtt                                                                                                      >  2:99666/1‑149 (MQ=255)
                                                          gCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCt                                                                                              <  1:99666/147‑1 (MQ=255)
                                                           cTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTca                                                                                            >  5:79684/1‑148 (MQ=255)
                                                               atcaAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCAcatca                                                                                       >  2:39877/1‑149 (MQ=255)
                                                                  aaGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCg                                                                                    >  3:260686/1‑149 (MQ=255)
                                                                   aGCTCAATGCACTGGGTATGCGAATTGCCGCCAACAAGGGCCGTTCGCTTCGCTGATTGCTTGCGTGTGCTAAAACCGCGGATCTGTGCGGGGTTTTTTTATGCCCGCGCTAAGGCGTGTCGTTCAACTTCGGTGGCTCACATCATCGc                                                                                   <  2:81676/149‑1 (MQ=255)
                                                                   aGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGc                                                                                   >  3:296394/1‑149 (MQ=255)
                                                                    gCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGcc                                                                                  >  3:9939/1‑149 (MQ=255)
                                                                     cTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGccc                                                                                 >  6:140533/1‑149 (MQ=255)
                                                                        aaTGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTc                                                                               <  5:140291/148‑1 (MQ=255)
                                                                         aTGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGa                                                                             <  5:126781/149‑1 (MQ=255)
                                                                             aCTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTgc                                                                         >  6:244911/1‑149 (MQ=255)
                                                                                 cGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCGGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTgcgc                                                                       <  4:209092/147‑1 (MQ=255)
                                                                                  gTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGa                                                                    <  4:9939/149‑1 (MQ=255)
                                                                                       cgcATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGaa                                                               <  6:79684/149‑1 (MQ=255)
                                                                                              ccgccAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTgcg                                                        <  8:91415/149‑1 (MQ=255)
                                                                                              ccgccAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGAGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTgcg                                                        >  8:281743/1‑149 (MQ=255)
                                                                                                   aacaaGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTgcgc                                                       <  2:177786/145‑1 (MQ=255)
                                                                                                   aacaaGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTgcgc                                                       >  1:177786/1‑145 (MQ=255)
                                                                                                       aGGGCCGTTCTCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGAc                                               <  2:142221/149‑1 (MQ=255)
                                                                                                       aGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGAc                                               <  2:255477/149‑1 (MQ=255)
                                                                                                        gggCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACg                                              >  8:19327/1‑149 (MQ=255)
                                                                                                           ccGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGG‑TTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTg                                          >  5:51528/1‑149 (MQ=255)
                                                                                                            cGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTg                                          >  1:200086/1‑149 (MQ=255)
                                                                                                              ttCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGc                                                                                                                    <  4:267996/73‑1 (MQ=255)
                                                                                                              ttCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGc                                                                                                                    >  3:267996/1‑73 (MQ=255)
                                                                                                              ttCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGG‑TTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTcc                                       <  6:51528/149‑1 (MQ=255)
                                                                                                                        tGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCa                                      <  8:305749/141‑1 (MQ=255)
                                                                                                                        tGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCa                                      >  7:305749/1‑141 (MQ=255)
                                                                                                                         gATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGt                                             <  4:232853/133‑1 (MQ=255)
                                                                                                                         gATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGt                                             >  3:232853/1‑133 (MQ=255)
                                                                                                                           ttGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACaaaa                            <  3:257913/148‑1 (MQ=255)
                                                                                                                                     ataaaaaaaCCCCGGACCGAGCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGAcgc                 >  5:327284/6‑149 (MQ=255)
                                                                                                                                     ataaaaaaaCCCCGGACCGAGCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGAcgc                 >  4:159187/6‑149 (MQ=255)
                                                                                                                                       aaaaaaaCCCCGGACCGAGCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGAcgcgc               >  1:149356/4‑149 (MQ=255)
                                                                                                                                         gAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACgcgcgc             >  4:57416/1‑149 (MQ=255)
                                                                                                                                         gAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACgcgcgc             >  4:217221/1‑149 (MQ=255)
                                                                                                                                           aaaCCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTgc                                                                         >  7:281706/1‑87 (MQ=255)
                                                                                                                                           aaaCCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTgc                                                                         <  8:281706/87‑1 (MQ=255)
                                                                                                                                            aaCCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATAATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTgg          >  3:173218/1‑149 (MQ=255)
                                                                                                                                             aCCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTGGc         <  7:19327/149‑1 (MQ=255)
                                                                                                                                               cccGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTGGCTc       >  2:49550/1‑149 (MQ=255)
                                                                                                                                                    cTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTGGCTCGGGCg  <  2:179558/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAACATCGCCACCTTTGAAGAAGATGGTGGTGCCTACAACCAGGCAGATGCTGCTGGCTTCATCAAGCTCAATGCACTGCGTATGCGCATTGCCGCCAACAAGGGCCGTTCGCTGCTCTGATTGCTGGCGTGTGCGAAAACCCCGGCTCGGTCCGGGGTTTTTTTATGCCCGCGCAAAGGCGGGTCGTTCAACTTCGGTGGCTCACATCATCGCCCTCGAGTGCGCTGATCGAAAGCTGCGCACATTGACGTCTGTCCAGATTACAAAAGCGCCAGACGCGCGCTGGCTCGGGCG  >  NC_002947/1248065‑1248361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑