Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,256,883	A→C	7.0%	noncoding (432/451 nt)	PP_mr14 ←	cspA regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,256,883	0	A	C	7.0%	172.5 / 4.7	57	noncoding (432/451 nt)	PP_mr14	cspA regulatory region
Reads supporting (aligned to +/- strand):  ref base A (29/24);  new base C (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

CATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTT  >  NC_002947/1256738‑1257030                                                                                                                                                   |                                                                                                                                                    cATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAgg                                                                                                                                                  >  5:176429/1‑149 (MQ=255) cATCACCGAAGACTGACCTGCCATACCGAGTTTGCCCGCTCACAGCGACTGCGCCGTACTTCAAGCCTGAGCAGTCCCTGAGGGAGCGGGCGAGCCCGCGAAGAGGACAGCGCCTAATTCGAGCAGTCAT‑AAAAAAACCCGCCAGAAgg                                                                                                                                                  >  2:110999/1‑149 (MQ=255)     aGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCACGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCgg                                                                                                                                               <  1:232821/148‑1 (MQ=255)          aGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                         >  8:160394/1‑149 (MQ=255)                aCCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGaaa                                                                                                                                   <  1:95824/149‑1 (MQ=255)                     ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                               <  1:216446/149‑1 (MQ=255)                     ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGtt                                                                                                                                            >  1:91627/1‑135 (MQ=255)                     ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTTCTGGCGGGtt                                                                                                                                            <  2:91627/135‑1 (MQ=255)                       aTACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAgg                                                                                                                             >  4:175095/1‑148 (MQ=255)                        tACCGAGTTTGCCCGCACACATCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGTCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGaa                                                                                                                           <  5:130204/149‑1 (MQ=255)                               tttGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGc                                                                                                                         >  6:133394/1‑144 (MQ=255)                                  gCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAtag                                                                                                                  >  1:141511/1‑146 (MQ=255)                                    ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACt                                                                                                               <  3:175095/149‑1 (MQ=255)                                    ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCCAGGAAGCTAGATCGACt                                                                                                               >  8:40873/1‑149 (MQ=255)                                      gCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACt                                                                                                               >  6:235175/1‑147 (MQ=255)                                       cacacaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                            <  6:176429/149‑1 (MQ=255)                                        acacaGCTACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGTAGCGGGCGAGCCCGCGAAGAGGCAAGCGCCTATTTCGAGCAGGAAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAgg                                                                                                           <  5:334042/149‑1 (MQ=255)                                           caGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                        >  4:109907/1‑149 (MQ=255)                                            aGCGACTGCGCCTTACTTGAAGCCTGTGCAGTCCCTGTGGGAGGGGGCGAGCCCGCTAAGTGGCCAGCGGCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCTGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGAt                                                                                                       <  7:199406/149‑1 (MQ=39)                                             gCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                         >  3:132238/1‑114 (MQ=255)                                             gCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGttttt                                                                                                                                         <  4:132238/114‑1 (MQ=255)                                                    cgcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCTCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAActt                                                                                               <  5:4789/149‑1 (MQ=255)                                                          aCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAg                                                                                         >  1:199106/1‑149 (MQ=255)                                                          aCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAg                                                                                         >  5:162001/1‑149 (MQ=255)                                                            ttGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGcc                                                                                       >  7:1872/1‑149 (MQ=255)                                                              gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCt                                                                                      >  2:308219/1‑148 (MQ=255)                                                                   cTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGc                                                                                <  4:155434/149‑1 (MQ=255)                                                                    tgtgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGcc                                                                               >  4:102605/1‑149 (MQ=255)                                                                     gtgCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCt                                                                              >  3:99962/1‑149 (MQ=255)                                                                          ctCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCt                                                                          <  3:191570/147‑1 (MQ=255)                                                                           tCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTgg                                                                        <  3:93220/149‑1 (MQ=255)                                                                             ccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCt                                                                          >  4:191570/1‑145 (MQ=255)                                                                                   ggAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCggg                                                                 <  7:160394/148‑1 (MQ=255)                                                                                    gAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCggg                                                                 >  3:16193/1‑147 (MQ=255)                                                                                     aGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAg                                                              >  8:246084/1‑149 (MQ=255)                                                                                        gggCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGCTCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGcga                                                           >  5:70051/1‑149 (MQ=255)                                                                                            gAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGaa                                                       <  2:141511/149‑1 (MQ=255)                                                                                              gCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCAATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                                    >  6:245322/1‑134 (MQ=255)                                                                                              gCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCAATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                                    <  5:245322/134‑1 (MQ=255)                                                                                                        agGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTg                                           <  8:1872/149‑1 (MQ=255)                                                                                                         ggCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTgg                                          <  7:246084/149‑1 (MQ=255)                                                                                                            cAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                       >  6:314156/1‑149 (MQ=255)                                                                                                            cAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCCCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                       <  5:235175/149‑1 (MQ=255)                                                                                                                     tttCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCa                                <  5:314156/147‑1 (MQ=255)                                                                                                                     tttCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAgg                              >  8:124553/1‑149 (MQ=255)                                                                                                                          aGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                         <  5:133394/149‑1 (MQ=255)                                                                                                                          aGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCCAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                         <  7:40873/149‑1 (MQ=255)                                                                                                                                    aaaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                 >  3:68190/1‑148 (MQ=255)                                                                                                                                     aaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGc                                                                                    >  1:241263/1‑80 (MQ=37)                                                                                                                                     aaaaaaCCCGCCTTCTGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGc                                                                                    <  2:241263/80‑1 (MQ=37)                                                                                                                                     aaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                 >  8:14186/1‑147 (MQ=255)                                                                                                                                            ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGa        >  3:98098/1‑149 (MQ=255)                                                                                                                                                cAGAAGGCGGGTTTATTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGACTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTCGCCTTCTTTCAGGTTTTTGAACCCGTCAGCTTGGATGGc    >  4:113161/1‑149 (MQ=255)                                                                                                                                                  gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                 >  1:262407/1‑134 (MQ=255)                                                                                                                                                  gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGt                 <  2:262407/134‑1 (MQ=255)                                                                                                                                                  gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCtt  <  3:102605/149‑1 (MQ=255)                                                                                                                                                  gAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCAACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCtt  >  6:73977/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCAT‑AAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGATGGCTT  >  NC_002947/1256738‑1257030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑