Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,273,533 A→C 7.2% H14Q (CAT→CAG)  PP_1114 ← SEC‑C domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,273,5330AC 7.2% 188.2 / 4.9 67H14Q (CAT→CAG) PP_1114SEC‑C domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base C (3/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAG  >  NC_002947/1273387‑1273681
                                                                                                                                                  |                                                                                                                                                    
tGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATg                                                                                                                                                    >  5:301028/1‑149 (MQ=255)
  gCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTTGTGATCATGGCCATgg                                                                                                                                                   <  6:114845/148‑1 (MQ=255)
    aCTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGAGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCCTgactg                                                                                                                                                >  5:57830/1‑145 (MQ=255)
              cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTAGATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGc                                                                                                                                      >  6:270132/1‑149 (MQ=255)
              cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGc                                                                                                                                      <  3:13273/149‑1 (MQ=255)
               ttcttcTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGc                                                                                                                                      >  3:176121/1‑148 (MQ=255)
                   tctcGCTACCGCACGGGCACGTTTAGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTTGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATg                                                                                                                                  >  4:95240/1‑148 (MQ=255)
                    ctcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGGGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCCTGACTGCAACCATGGCCATg                                                                                                                                  <  6:57830/147‑1 (MQ=255)
                    ctcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGaa                                                                                                                                >  1:124130/1‑149 (MQ=255)
                     tcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAg                                                                                                                                             <  5:23109/135‑1 (MQ=255)
                     tcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAg                                                                                                                                             >  6:23109/1‑135 (MQ=255)
                      cGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACg                                                                                                                              >  3:288943/1‑149 (MQ=255)
                        cTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTTCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTg                                                                                                                            <  2:124130/149‑1 (MQ=255)
                           ccGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGGGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTggg                                                                                                                          <  8:262328/148‑1 (MQ=255)
                           ccGCACGGGCACCGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTgg                                                                                                                           <  3:72892/147‑1 (MQ=255)
                             gcacgggcacggGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTCATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGgtt                                                                                                                       >  5:283639/1‑149 (MQ=255)
                                  ggctcgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTgg                                                                                                                  <  5:19698/149‑1 (MQ=255)
                                  ggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTgg                                                                                                                  <  3:8367/149‑1 (MQ=255)
                                       gggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTGGGGACAt                                                                                                             >  4:53623/1‑149 (MQ=255)
                                            gTTACGTCCAACGTCCTTCATGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCa                                                                                                         <  3:209765/148‑1 (MQ=255)
                                            gTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAg                                                                                                        <  6:301028/149‑1 (MQ=255)
                                             ttACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                                                                                                       <  2:958/149‑1 (MQ=255)
                                                 ggCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCa                                                                                                   >  8:221857/1‑149 (MQ=255)
                                                    cAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTc                                                                                                >  6:34744/1‑149 (MQ=255)
                                                            ttCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                                                                                                       <  6:121450/134‑1 (MQ=255)
                                                            ttCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                                                                                                       >  5:121450/1‑134 (MQ=255)
                                                            ttCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGGGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATg                                                                                         >  2:230723/1‑148 (MQ=255)
                                                              cAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGcc                                                                                                                                     >  7:267613/1‑102 (MQ=255)
                                                              cAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGcc                                                                                                                                     <  8:267613/102‑1 (MQ=255)
                                                              cAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTaa                                                                                      >  7:293741/1‑149 (MQ=255)
                                                               aGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTaa                                                                                      <  7:221857/148‑1 (MQ=255)
                                                               aGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAAt                                                                                     >  1:16050/1‑149 (MQ=255)
                                                                          tATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCCTGACTGCAACCATGGCCa                                                                                                                                    >  5:227629/1‑91 (MQ=37)
                                                                          tATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCCTGACTGCAACCATGGCCa                                                                                                                                    <  6:227629/91‑1 (MQ=37)
                                                                          tATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                          <  5:34744/149‑1 (MQ=255)
                                                                          tATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                          <  4:176121/149‑1 (MQ=255)
                                                                            tCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTa                                                                         >  4:157322/1‑148 (MQ=255)
                                                                               gCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGTGGTTGTTGGGACa                                                                                                              <  8:144262/108‑1 (MQ=255)
                                                                               gCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACa                                                                                                              >  7:144262/1‑108 (MQ=255)
                                                                                 tCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTcac                                                                   >  4:62315/1‑149 (MQ=255)
                                                                                 tCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGAAGGGTCACTCCGGTATGTAATCGTCGTGGATTATCTcac                                                                   >  8:164829/1‑149 (MQ=255)
                                                                                       tggCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                          >  7:216653/1‑136 (MQ=255)
                                                                                       tggCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                          <  8:216653/136‑1 (MQ=255)
                                                                                         gCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTgcgc                                                           <  6:283639/149‑1 (MQ=255)
                                                                                         gCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTgcgc                                                           >  7:120621/1‑149 (MQ=255)
                                                                                                   cAGTGGGGGCCGTGTACCTGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGc                                                  <  7:144909/148‑1 (MQ=255)
                                                                                                   cAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTgcgc                                                           >  7:262032/1‑139 (MQ=255)
                                                                                                   cAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTgcgc                                                           <  8:262032/139‑1 (MQ=255)
                                                                                                   cAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGc                                                  <  2:16050/148‑1 (MQ=255)
                                                                                                    aGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAg                                                >  4:1108/1‑149 (MQ=255)
                                                                                                    aGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAg                                                <  3:95240/149‑1 (MQ=255)
                                                                                                          ggCCATGTACACGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTccc                                           >  5:109254/1‑148 (MQ=255)
                                                                                                               tgtACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCTTCGGGGATTATCTAACCACTGCGCGAGATGTGAAGGTCACgatgga                                     >  7:285950/1‑149 (MQ=255)
                                                                                                               tgtACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGttgttg                                                                                                                   <  2:121223/71‑1 (MQ=255)
                                                                                                               tgtACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGttgttg                                                                                                                   >  1:121223/1‑71 (MQ=255)
                                                                                                               tgtACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCgatgga                                     <  8:120621/149‑1 (MQ=255)
                                                                                                                 tACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCgatggatg                                   <  3:157322/149‑1 (MQ=255)
                                                                                                                 tACACGGCCGTGGTCGTGGTGGTTATCATGGCCTTGGTGCCCGTCCGCGCCATGAACGTGGGGTTGTTGGGACATTCCAGGGTCACTCGGGTATTTAATCGGGGGGGATTATCTCCCCACTGCGCCAGATGTGCCGGTCCCgatggag                                    <  6:109254/148‑2 (MQ=255)
                                                                                                                   cATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGTGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGa                                  <  7:164829/148‑1 (MQ=255)
                                                                                                                         cgtggtcgtggtGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAAccc                           >  6:319170/1‑149 (MQ=255)
                                                                                                                          gtggtcgtggtGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCg                          >  3:113990/1‑149 (MQ=255)
                                                                                                                                      gtgATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCg              >  6:283071/1‑149 (MQ=255)
                                                                                                                                        gttCATGGCCCTGACTGCAACCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGCGATTATCTAACCACTGCGCGAGATGTGCAGGTCCCGCAGGATGAGCACCCCGGTCGCCAGTTCGcc            >  4:214680/3‑149 (MQ=255)
                                                                                                                                             tGGCCATGGTTGCAGTCAGGGCCAGGAACGGGGGGTTGGTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCa       <  3:53623/149‑1 (MQ=255)
                                                                                                                                               gCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGGGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAgg     >  4:302662/1‑149 (MQ=255)
                                                                                                                                                 cATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGa   <  3:62315/149‑1 (MQ=255)
                                                                                                                                                 cATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACAACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAgg     >  7:303015/1‑147 (MQ=255)
                                                                                                                                                  aTGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAg  >  3:196557/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGGTGATCATGGCCATGGTTGCAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAG  >  NC_002947/1273387‑1273681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑