Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,293,918 T→C 7.9% L156P (CTG→CCG)  pdxH → pyridoxine/pyridoxamine 5'‑phosphate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,293,9180TC 7.9% 140.1 / 2.3 52L156P (CTG→CCG) pdxHpyridoxine/pyridoxamine 5'‑phosphate oxidase
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

TCAGGTGCGCATCGAGGGCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTG  >  NC_002947/1293772‑1294063
                                                                                                                                                  |                                                                                                                                                  
tCAGGTGCGCATCGAGGGCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCCgg                                                                                                                                                  >  7:26801/1‑149 (MQ=255)
tCAGGTGCGCATCGAGGGCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCCgg                                                                                                                                                  >  3:9114/1‑149 (MQ=255)
     tGCGCATCGAGGGCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAg                                                                                                                                             <  8:301238/149‑1 (MQ=255)
                 gCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAgcg                                                                                                                                 <  6:86721/149‑1 (MQ=255)
                     ggTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGtt                                                                                                                             >  1:157621/1‑149 (MQ=255)
                          aGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTcc                                                                                                                        <  4:33531/149‑1 (MQ=255)
                          aGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTcc                                                                                                                        >  7:32420/1‑149 (MQ=255)
                                tCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCCGGTAAAGGCCACCGAAGCGCGTTTCTCCGACAcc                                                                                                                  <  8:26801/149‑1 (MQ=255)
                                 cACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCCGGTAAAGGCCACCGAAGCGCGTTTCTCCGACAcc                                                                                                                  <  4:9114/148‑1 (MQ=255)
                                    ggCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCAGGTAAAGGCCACCGAAGCGCGTTTCTcc                                                                                                                        >  7:168121/1‑139 (MQ=255)
                                                        actacCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTgg                                                                                         >  4:148425/1‑149 (MQ=255)
                                                         ctacCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTgg                                                                                         <  3:148425/148‑1 (MQ=255)
                                                            ccAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGCCACCCAGCCG‑CACTGCCCTGAGCATTgggggg                                                                                     >  7:169954/1‑149 (MQ=255)
                                                            ccAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAg                                                                                                               <  3:60582/124‑1 (MQ=255)
                                                            ccAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATAgggggg                                                                                     >  8:59697/1‑149 (MQ=255)
                                                            ccAGGTACGCACGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGAGTGATTGCCTGGCTTGAAGAGCTGGAGGGCATGGTAAAGGCCACCGAAGCGCTGTTCTCCTACACACAg                                                                                                               >  4:60582/1‑124 (MQ=255)
                                                               ggTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGa                                                                                   >  7:105492/1‑148 (MQ=255)
                                                                gTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGTCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGacac                                                                                                                   <  1:220753/116‑1 (MQ=255)
                                                                gTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGacac                                                                                                                   >  2:220753/1‑116 (MQ=255)
                                                                gTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATa                                                                                 >  2:44526/1‑149 (MQ=255)
                                                                    gCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTggggg                                                                                      <  3:189870/140‑1 (MQ=255)
                                                                    gCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTggggg                                                                                      >  4:189870/1‑140 (MQ=255)
                                                                    gCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTATCCGACACCCAGCCG‑CACTGCCATGAGCATTGGGGGGGAAGCCGt                                                                             >  4:248319/1‑149 (MQ=255)
                                                                     cccGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CGCTGCCCTGAGCATTg                                                                                          <  5:295029/135‑1 (MQ=255)
                                                                     cccGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CGCTGCCCTGAGCATTg                                                                                          >  6:295029/1‑135 (MQ=255)
                                                                         ttGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCGCCACTGCCCTGAGCATTGGGGGGGATACCGTttgt                                                                         <  1:44526/149‑1 (MQ=255)
                                                                              cAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGccc                                                                    >  4:150727/1‑148 (MQ=255)
                                                                               aGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCTGACACCCAGCCG‑CACTGCCCTGa                                                                                                <  1:214098/119‑1 (MQ=255)
                                                                               aGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCTGACACCCAGCCG‑CACTGCCCTGa                                                                                                >  2:214098/1‑119 (MQ=255)
                                                                               aGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGa                                                                  <  8:23427/149‑1 (MQ=255)
                                                                                   gCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTg                                                                                                 <  5:256531/114‑1 (MQ=255)
                                                                                   gCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTg                                                                                                 >  6:256531/1‑114 (MQ=255)
                                                                                        ggggCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATc                                                           <  2:157621/147‑1 (MQ=255)
                                                                                           gCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGtt                                                      <  3:150727/149‑1 (MQ=255)
                                                                                                  cTTCGCCACAGAGCCGCGTGATTGCCGGGTGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAg                                               >  6:127599/1‑149 (MQ=255)
                                                                                                  cTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAg                                               >  1:194440/1‑149 (MQ=255)
                                                                                                        cacaGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTG‑CCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGgggcggg                                        <  8:32420/149‑1 (MQ=255)
                                                                                                               gcgcgTGATTGCCGGGCGTGAAGAGGTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAgtc                                  <  3:248319/148‑1 (MQ=255)
                                                                                                                 gcgTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAgtcgt                                <  2:194440/149‑1 (MQ=255)
                                                                                                                   gTGATTGCCGGGTGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCt                              <  5:127599/149‑1 (MQ=255)
                                                                                                                     gATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTg                             >  4:176946/1‑148 (MQ=255)
                                                                                                                           cGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTgg                                                  <  7:203773/121‑1 (MQ=255)
                                                                                                                           cGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTgg                                                  >  8:203773/1‑121 (MQ=255)
                                                                                                                           cGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATc                      <  3:176946/149‑1 (MQ=255)
                                                                                                                                   aaGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCaa                                     >  7:69910/1‑126 (MQ=255)
                                                                                                                                   aaGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCaa                                     <  8:69910/126‑1 (MQ=255)
                                                                                                                                     gagCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTa            <  8:169954/149‑1 (MQ=255)
                                                                                                                                       gCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTgggggg                                                                                     <  2:239281/74‑1 (MQ=255)
                                                                                                                                       gCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTgggggg                                                                                     >  1:239281/1‑74 (MQ=255)
                                                                                                                                          ggAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGc       >  3:9817/1‑149 (MQ=255)
                                                                                                                                              ggCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCtggt   <  4:9817/149‑1 (MQ=255)
                                                                                                                                                ccTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGTGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCtggtg  >  6:287728/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCAGGTGCGCATCGAGGGCCGGGTGCAGAAGGTCACGGCGCAGGAGTCGGATGCCTACTACCAGGTACGCCCGTTGGGCAGTCGCCTGGGGGCCTGGGCTTCGCCACAGAGCCGCGTGATTGCCGGGCGTGAAGAGCTGGAGGGCCTGGTAAAGGCCACCGAAGCGCGTTTCTCCGACACCCAGCCG‑CACTGCCCTGAGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTG  >  NC_002947/1293772‑1294063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑