| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,313,636 | G→C | 5.2% | R341G (CGC→GGC) | rapA ← | RNA polymerase‑associated protein RapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,313,636 | 0 | G | C | 5.2% | 249.2 / 2.9 | 77 | R341G (CGC→GGC) | rapA | RNA polymerase‑associated protein RapA |
| Reads supporting (aligned to +/- strand): ref base G (32/41); new base C (2/2); total (34/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
TCGCCGTCGCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAGGCCGTATTCGG > NC_002947/1313488‑1313782 | tCGCCGTCGCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGcgcg > 5:301135/1‑149 (MQ=255) tCGCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGc > 7:307236/1‑149 (MQ=255) gCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCgg > 4:105891/1‑149 (MQ=255) gCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCgg > 5:147455/1‑149 (MQ=255) cTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑c > 5:179849/1‑149 (MQ=255) cGCTGTCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCCGCGCCGAAAGCga < 5:175012/143‑2 (MQ=255) cGCTGTCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCCGCGCCGAAAGCga > 6:175012/1‑142 (MQ=255) cGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGt > 3:282657/1‑149 (MQ=255) cGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCCGCGCCGAAAGCga > 1:295020/1‑142 (MQ=255) cGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCCGCGCCGAAAGCga < 2:295020/143‑2 (MQ=255) tGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGGATCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGt < 3:55697/149‑1 (MQ=255) tGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGt < 8:7591/149‑1 (MQ=255) ccAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTg < 6:179849/148‑1 (MQ=255) gcagGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGaaa‑‑a < 7:258471/149‑1 (MQ=255) ggCTTCGCCTTCGACACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGt > 7:176025/1‑149 (MQ=255) gCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGtt > 6:98109/1‑149 (MQ=255) cTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTg < 6:15526/149‑1 (MQ=255) cGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTggg < 5:98109/148‑1 (MQ=255) cGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTggg < 5:278643/148‑1 (MQ=255) cGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTggg < 4:282657/148‑1 (MQ=255) cGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACAGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTCgggg > 8:214449/1‑149 (MQ=255) gCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGt < 6:301135/149‑1 (MQ=255) ccTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTc > 4:69173/1‑149 (MQ=255) cACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAgg < 3:105891/149‑1 (MQ=255) aGGAAGGCCTGGATGGTGGCGTGGGCGTTGGGCGAAAGAGGGCCCTCGTCGTGCTGTTCCTGCACCGCCTCGGCTACCGGGCGGTTGTGCTCGCTTTGGGCGCGGAAAGCGG‑‑CCAGGTGGTCAAT‑‑AC‑GGTTGGGGTCGAGTAGGCGCAg < 7:47815/149‑1 (MQ=255) aGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGACTCGGATACCGGGCGGTAGTGCTCGCTTTCGGAGCGGAAAGCGG‑‑CCAGGTGGTGAAA‑‑GC‑GGTTGGGGTCGAGTAGGCGCAg > 6:102865/1‑149 (MQ=255) aGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAggcgg < 6:147455/149‑1 (MQ=255) aGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAggcgg < 7:149918/149‑1 (MQ=255) gCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAggcggg > 6:249031/1‑149 (MQ=255) aGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGaa < 4:156224/149‑1 (MQ=255) gATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGt < 4:250023/149‑1 (MQ=255) gATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGt < 4:254393/149‑1 (MQ=255) gTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCt > 3:207092/1‑149 (MQ=255) tGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTg > 7:4688/1‑149 (MQ=255) gggCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTgg < 6:10271/149‑1 (MQ=255) ggCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTgg < 4:207092/148‑1 (MQ=255) ccTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGccc > 4:83731/1‑149 (MQ=255) gggCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAgc < 8:176025/148‑1 (MQ=255) ggCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTg < 3:83731/149‑1 (MQ=255) ggCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTg < 8:4688/149‑1 (MQ=255) gCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTgg > 2:201965/1‑92 (MQ=255) ccTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGTCTGTCCTg < 5:196141/127‑1 (MQ=255) ccTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTg > 6:196141/1‑127 (MQ=255) tcgtcgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg < 7:124559/117‑1 (MQ=255) tcgtcgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg > 8:124559/1‑117 (MQ=255) gtcgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg > 2:213436/1‑115 (MQ=255) gtcgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg < 1:213436/115‑1 (MQ=255) gtcgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGt < 8:307236/147‑1 (MQ=255) gtcgAGCAGTTCATGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAATCGT‑‑CCAGGTCGTGAAT‑‑AC‑GGTTGGGGTCGAGTTGGCGCAGGCGGGCGAATTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCa < 7:49683/149‑1 (MQ=255) cgAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGc < 5:249031/149‑1 (MQ=255) gAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGc > 6:82771/1‑148 (MQ=255) aGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCaa < 7:37568/149‑1 (MQ=255) aGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGGCACCAGGTCGTTAAACTCCAGGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGc < 1:103303/149‑1 (MQ=255) ttCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTg < 4:222210/76‑1 (MQ=255) cTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCGGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGc < 4:80586/149‑1 (MQ=255) gCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGccc > 3:140554/1‑149 (MQ=255) cACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCg > 1:326970/1‑149 (MQ=255) cGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGaa > 3:27940/1‑149 (MQ=255) ccTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTT‑GGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCa < 2:8567/149‑1 (MQ=255) ccTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTCGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATc < 7:214449/149‑1 (MQ=255) cTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGAGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTgg < 5:106627/149‑1 (MQ=255) cTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGAGG‑‑CCAGGTAGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTgg > 6:106627/1‑149 (MQ=255) aCCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACtt > 8:13425/1‑145 (MQ=255) aCCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACtt < 7:13425/145‑1 (MQ=255) aCCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACtt < 7:18180/145‑1 (MQ=255) aCCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACtt > 8:18180/1‑145 (MQ=255) cGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg < 5:129809/85‑1 (MQ=255) cGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTgg > 6:129809/1‑85 (MQ=255) gCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGAc < 5:112532/148‑1 (MQ=255) cTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGAc > 3:225010/1‑147 (MQ=255) tCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAg > 6:93580/1‑149 (MQ=255) tCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGCAGGTCAGCAAAAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGAc > 6:112532/1‑146 (MQ=255) cGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAgg > 6:135833/1‑149 (MQ=255) gCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAgg < 4:225010/148‑1 (MQ=255) gCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAg < 3:28610/147‑1 (MQ=255) tttCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCgggg > 4:55616/1‑92 (MQ=255) tttCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCgggg < 3:55616/92‑1 (MQ=255) gcgcGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAGGCCGTATTc > 5:334226/1‑149 (MQ=255) gcgGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAGGCCGTATTCgg > 4:222066/1‑149 (MQ=255) | TCGCCGTCGCTGACCGCTGCCAGCAGGGCTTCGCCTTCGGCACCCAGGAAGCCCAGGATGGTGGCGTGGGCCTTGGGCGACAGACGGCCCTCGTCGAGCAGTTCCTGCACCGCCTCGGCTACCGGGCGGTAGTGCTCGCTTTCGGCGCGGAAAGCGG‑‑CCAGGTCGTGAAA‑‑AC‑GGTTGGGGTCGAGTAGGCGCAGGCGGGCGAAGTGGCTGTCCTGGCCCAGCTGCTCGGGGGTGGCGGTCAGCAACAGTACGCCCGGAATCACTTGGGCCAGTTGCTCGACCAGGCCGTATTCGG > NC_002947/1313488‑1313782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |