| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,356,405 | G→C | 13.1% | G94A (GGC→GCC) | PP_1181 → | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,356,405 | 0 | G | C | 13.1% | 157.6 / 11.8 | 59 | G94A (GGC→GCC) | PP_1181 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (24/27); new base C (4/4); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGA > NC_002947/1356261‑1356546 | tcGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTg < 7:25153/149‑1 (MQ=255) agccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCt < 4:33250/149‑1 (MQ=255) ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg < 3:281200/149‑1 (MQ=255) ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg < 1:58683/149‑1 (MQ=255) ccTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCACCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcgac > 1:234118/1‑148 (MQ=255) cTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcga < 4:256502/137‑1 (MQ=255) cTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcga > 3:256502/1‑137 (MQ=255) gACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCa > 6:293742/1‑148 (MQ=255) ccTGATGCTCCCGGGCGAGGACGGCCTGAGCATCTGCCACCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc < 2:234118/149‑1 (MQ=255) cTCCCCGGCGAGGACGCCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgc < 2:97190/148‑1 (MQ=255) ccccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACt < 2:209217/128‑1 (MQ=255) ccccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACt > 1:209217/1‑128 (MQ=255) ccccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGcc > 6:25868/1‑149 (MQ=255) cGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCt > 3:55852/1‑149 (MQ=255) cggACGGCCTGAGCATCGGCCGCCGGGTGCGCAGCCAGTACCCGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc < 6:186195/131‑1 (MQ=255) aGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc > 5:186195/1‑132 (MQ=255) aGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtg < 4:254289/149‑1 (MQ=255) gACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGCCCAGGTCCAGGCCCTGGACCTggt < 7:202274/107‑2 (MQ=255) gACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGCCCAGGTCCAGGCCCTGGACCTggt > 8:202274/1‑106 (MQ=255) ccTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg > 7:228027/1‑149 (MQ=255) ccTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg > 1:119494/1‑149 (MQ=255) cTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGCCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTACGCCCACGCTTGCTGCTGGc > 8:152287/1‑149 (MQ=255) tGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCAGTTCGACCAAGCTTGCTACGGGCa > 6:228046/1‑149 (MQ=255) aGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCCCCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc < 8:189309/148‑1 (MQ=255) cATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgc > 6:102578/1‑125 (MQ=255) tCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTggtc > 7:263877/1‑93 (MQ=255) tCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTggtc < 8:263877/95‑3 (MQ=255) cTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc < 3:212415/88‑1 (MQ=255) cTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc > 4:212415/1‑88 (MQ=255) ccgccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATcc < 5:153854/146‑1 (MQ=255) ccgccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAg < 5:293742/148‑1 (MQ=255) ccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGccc < 6:301553/149‑1 (MQ=255) ggTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCctgc > 6:82513/1‑149 (MQ=255) gcAGCCTTTACCAGGGGCCTATCTTCATGCTCACCGGGCGCATTTATTAACTGGACCAGGTCATGGGCCTGGACTTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTa < 4:6815/132‑1 (MQ=255) ggCCGAACCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTg < 8:228027/149‑1 (MQ=255) cggATCGTCATGCTCACCGCGCGCTGTGATGATCTGCACCTGGGGCAGGGCGTGGACGTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGa < 5:25868/147‑1 (MQ=255) tCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAg > 5:152979/1‑149 (MQ=255) tGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTGGGCGCAGACGACTACGTCTGCAAACCCGTCCGCCCACCCTTGCTGCTGGCACGTATCAAGGCCCtgctgc > 2:162622/1‑120 (MQ=255) tCACCGCTCTCAGTTATGAAGTGGACCTGGTCCAGGGCCTTGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACa < 4:90194/138‑1 (MQ=255) tCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCATGCTGCGCCGCAGCGAAACCGTGGACa > 3:90194/1‑138 (MQ=255) aCCGCGCGCAGTTTTGACCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGa < 2:119494/149‑1 (MQ=255) aGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCtt > 4:80654/1‑149 (MQ=255) aGTGATGAACTGCACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCtt < 5:228046/149‑1 (MQ=255) aGTGAGGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCtt < 2:137426/149‑1 (MQ=255) ccTGGACCTGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg > 4:35652/2‑137 (MQ=255) ccTGGACCTGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg < 3:35652/136‑1 (MQ=255) cTGGACCAGGCCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTACGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCt < 7:152287/149‑1 (MQ=255) tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCctg < 7:267808/92‑1 (MQ=255) tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCctg > 8:267808/1‑92 (MQ=255) ggACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGGTGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGc < 5:82513/149‑1 (MQ=255) ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGt > 6:189460/1‑139 (MQ=255) ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGt < 5:189460/139‑1 (MQ=255) cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt > 7:103143/1‑149 (MQ=255) cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt > 1:128056/1‑149 (MQ=255) tCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGcc > 6:92042/1‑80 (MQ=255) tCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGcc < 5:92042/80‑1 (MQ=255) tCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGa > 3:92818/1‑148 (MQ=255) ccAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg < 2:124158/65‑1 (MQ=255) ccAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg > 1:124158/1‑65 (MQ=255) | TCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGA > NC_002947/1356261‑1356546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |