Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,078	C→A	6.1%	V75F (GTC→TTC)	PP_1204 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,078	0	C	A	6.1%	216.0 / 3.6	66	V75F (GTC→TTC)	PP_1204	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base A (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGT  >  NC_002947/1382939‑1383221                                                                                                                                            |                                                                                                                                                cccAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAgcg                                                                                                                                        >  8:159378/1‑149 (MQ=255)  ccAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAgcgc                                                                                                                                       >  2:108106/1‑149 (MQ=255)   cAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCt                                                                                                                                      >  1:80900/1‑149 (MQ=255)   cAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAAGTCACCGAATTCGAAgtgac                                                                                                                                      >  3:278219/1‑145 (MQ=255)     aGGCGAAGACGTTGACCACACTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGATGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCttt                                                                                                                                    >  2:180595/1‑149 (MQ=255)      ggCGACGACGTTGACCACATTCGCGCCGACACCCCCCACCACGGCCACTGCACCCAGCGGGATCTCCTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCtttt                                                                                                                                   <  8:243742/149‑1 (MQ=255)      ggCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAAGTCACCGAATTCGAAgtgacccg                                                                                                                                   <  4:278219/149‑8 (MQ=255)                     cacaTTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGca                                                                                                                    >  7:88751/1‑149 (MQ=255)                       caTTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGcaca                                                                                                                  <  5:85605/149‑1 (MQ=255)                            gcgcCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCAcgcg                                                                                                                      >  2:58234/1‑140 (MQ=255)                            gcgcCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCAcgcg                                                                                                                      <  1:58234/140‑1 (MQ=255)                                      ccGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCa                                                                                                                              >  5:122751/1‑122 (MQ=255)                                      ccGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCa                                                                                                                              <  6:122751/122‑1 (MQ=255)                                      ccGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGCGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAg                                                                                                    >  6:277755/1‑148 (MQ=255)                                        gCCACCACGGCCACTGCACCCAGCTGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTc                                                                                                 <  8:88751/149‑1 (MQ=255)                                                ggCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCt                                                                                         <  2:236247/149‑1 (MQ=255)                                                ggCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCt                                                                                         <  1:108106/149‑1 (MQ=255)                                                  ccACTGCACCCAGCGGTATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       <  3:30967/149‑1 (MQ=255)                                                  ccACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       >  5:243411/1‑149 (MQ=255)                                                  ccACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       >  4:187485/1‑149 (MQ=255)                                                  ccACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       >  1:19975/1‑149 (MQ=255)                                                   cACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCg                                                                                      >  1:292700/1‑149 (MQ=255)                                                            cAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGa                                                                             <  2:80900/149‑1 (MQ=255)                                                                   aTCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       <  6:216653/132‑1 (MQ=255)                                                                   aTCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       >  5:216653/1‑132 (MQ=255)                                                                      tcGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTg                                                                   >  5:332289/1‑149 (MQ=255)                                                                        gTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTg                                                                   >  5:172996/1‑147 (MQ=255)                                                                        gTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTg                                                                   >  3:209436/1‑147 (MQ=255)                                                                         tAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAg                                                                <  3:187485/149‑1 (MQ=255)                                                                           gATCTCCCGCCAGGGTTGGTAAGGCTTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAgg                                                               <  5:277755/148‑1 (MQ=255)                                                                           gATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAgg                                                               <  6:332289/148‑1 (MQ=255)                                                                            aTCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       >  4:89983/1‑123 (MQ=255)                                                                            aTCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTc                                                                                       <  3:89983/123‑1 (MQ=255)                                                                               tcCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATg                                                          >  7:289830/1‑149 (MQ=255)                                                                               tcCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATg                                                          >  2:233328/1‑149 (MQ=255)                                                                                cccGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGc                                                         >  5:23013/1‑149 (MQ=255)                                                                                  ccgctgggTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTc                                                       >  7:256113/6‑149 (MQ=255)                                                                                    ccAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTc                                                       >  7:39179/1‑147 (MQ=255)                                                                                       gggTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCTGCTCGACGACATCGATCTGCAGGGTATGCTCGAg                                                    >  6:91350/1‑147 (MQ=255)                                                                                       gggTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCa                                                  <  5:91350/149‑1 (MQ=255)                                                                                             gTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTgc                                            >  7:244949/1‑149 (MQ=255)                                                                                             gTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTgc                                            >  7:214108/1‑149 (MQ=255)                                                                                              tAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTgcg                                           <  6:243411/149‑1 (MQ=255)                                                                                               aaGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCa                                                  >  4:218974/1‑141 (MQ=255)                                                                                               aaGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCa                                                  <  3:218974/141‑1 (MQ=255)                                                                                                    gtgtAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGAc                                     <  8:289830/149‑1 (MQ=255)                                                                                                      gtAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGAcgc                                   >  7:47150/1‑149 (MQ=255)                                                                                                       tAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGAcgcg                                  >  1:119819/1‑149 (MQ=255)                                                                                                            gTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGctcct                             >  4:230501/1‑149 (MQ=255)                                                                                                               aTAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAg                                                                >  6:73467/1‑111 (MQ=255)                                                                                                               aTAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAg                                                                <  5:73467/111‑1 (MQ=255)                                                                                                               aTAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCAt                          <  6:23013/149‑1 (MQ=255)                                                                                                                tAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATg                         >  8:276639/1‑149 (MQ=255)                                                                                                                  acgacgGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGCCGCGCTCCTCATgg                        <  7:276639/148‑1 (MQ=255)                                                                                                                  acgacgGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGt                       <  8:256113/149‑1 (MQ=255)                                                                                                                      aaaagcgcTTCGAAGTCACCGAATTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgct                   >  6:268599/8‑149 (MQ=255)                                                                                                                        aagcgcTTCGAAGTCACCGAATTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTcgctgc                 <  5:268599/144‑1 (MQ=255)                                                                                                                             cTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            >  7:129091/1‑149 (MQ=255)                                                                                                                             cTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            <  2:119819/149‑1 (MQ=255)                                                                                                                             cTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            <  8:214108/149‑1 (MQ=255)                                                                                                                             cTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            <  6:172996/149‑1 (MQ=255)                                                                                                                              ttCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGCCGCGCTCCTCATGGTCGCTGCGCTggg           >  6:90426/1‑149 (MQ=255)                                                                                                                                cGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTggg           <  1:193997/147‑1 (MQ=255)                                                                                                                                   aTTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            >  8:202779/1‑143 (MQ=255)                                                                                                                                   aTTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTgg            <  7:202779/143‑1 (MQ=255)                                                                                                                                       ggTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATTGTCGCTGCGCTGGGACATCTGGt  <  8:47150/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                CCCAAGGCGAAGACGTTGACCACATTGGCGCCGACACCCGCCACCACGGCCACTGCACCCAGCGGGATCTCGTAGATCTCCCGCCAGGGTTGGTAAGGCGTGTAGCGGTCATAACGACGGGTCACTTCGAATTCGGTGACTTCGAAGCGCTTTTGCTCATGCACGCGCACACGTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGT  >  NC_002947/1382939‑1383221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑