| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,403,590 | G→A | 17.8% | intergenic (‑56/+25) | PP_1227 ← / ← PP_1228 | membrane protein/methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,403,590 | 0 | G | A | 17.8% | 59.3 / 4.9 | 28 | intergenic (‑56/+25) | PP_1227/PP_1228 | membrane protein/methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base G (10/13); new base A (2/3); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
CGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGG > NC_002947/1403447‑1403736 | cGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGGGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGccc < 2:307885/149‑1 (MQ=255) gATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCcg > 2:234015/1‑149 (MQ=255) aaCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGaa < 7:246441/149‑1 (MQ=255) aCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAag < 3:185138/149‑1 (MQ=255) cacgggggcggcGATCGAGGTGCCTAAACGCCCTTGTTCGCTTGTGATGTCGAAGAAAGTGGATCGGCTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAaga < 3:232434/143‑1 (MQ=255) aGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag < 6:106856/149‑1 (MQ=255) gggTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGCGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAagag < 1:234015/149‑1 (MQ=255) ggTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGc < 5:317899/149‑1 (MQ=255) gTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGTTGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGcc < 1:274251/149‑1 (MQ=255) tAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAAGAGGccc > 8:127136/1‑130 (MQ=255) tAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAAGAGGccc < 7:127136/130‑1 (MQ=255) cTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCa < 3:244857/149‑1 (MQ=255) tCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTgg > 5:260368/1‑149 (MQ=255) gaagaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGtt > 7:206785/1‑149 (MQ=255) aagaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAgcgc > 5:115331/1‑136 (MQ=255) aagaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAgcgc < 6:115331/136‑1 (MQ=255) aagaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTg < 7:67880/149‑1 (MQ=255) gaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGc < 6:260368/149‑1 (MQ=255) atatGTTTTGCTCAACAATAACGTTT‑GTCCGGTGCCTTGCAGGGCCTCTTCGGGGGCGTGCCCGCGTAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATg < 5:182633/149‑1 (MQ=255) tgtgCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGc < 8:206785/148‑1 (MQ=255) tGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTCGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCt > 6:316389/1‑149 (MQ=255) ggCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACg > 1:156454/1‑149 (MQ=255) tctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGAGTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtctc > 8:82996/1‑149 (MQ=255) ttCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCg > 5:142630/1‑148 (MQ=255) ttCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCg > 8:7325/1‑148 (MQ=255) ttCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCg > 2:274671/1‑148 (MQ=255) gggCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCGGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGcc > 5:58438/1‑149 (MQ=255) gCGTGCTCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCgg < 6:329739/149‑1 (MQ=255) | CGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATG‑GTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGG > NC_002947/1403447‑1403736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |