Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,407,796	G→A	6.6%	V83V (GTG→GTA)	PP_1230 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,407,796	0	G	A	6.6%	179.2 / 2.2	60	V83V (GTG→GTA)	PP_1230	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/33);  new base A (2/2);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

TGGACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTGCAGAAGGATGACGCCGTTG  >  NC_002947/1407648‑1407941                                                                                                                                                      |                                                                                                                                                  tGGACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑Atgtg                                                                                                                                                   >  4:191125/1‑149 (MQ=255)   gACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑Atgtg                                                                                                                                                   <  3:73314/147‑1 (MQ=255)   gACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑Atgtg                                                                                                                                                   <  8:4008/147‑1 (MQ=255)   gACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCGTCAGCAGCACTGCCCAGTACAGGCTCAAGGCTCGCGGCTTCA‑ATGTggg                                                                                                                                                 >  8:235173/1‑149 (MQ=255)     cAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTgggg                                                                                                                                                >  8:168261/1‑148 (MQ=255)      aaCGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCTTCGAGCTTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCa                                                                                                                                              <  8:256382/149‑1 (MQ=255)          cccTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGc                                                                                                                                          <  2:244128/149‑1 (MQ=255)               gCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAg                                                                                                                                       <  7:123734/147‑1 (MQ=255)                cACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGt                                                                                                                                     <  1:321107/148‑1 (MQ=255)                cACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGt                                                                                                                                     >  4:194653/1‑148 (MQ=255)                cACGCAGCAACGCCGAACTAACAAAGGGACTGTTCAGTACCGGTGGTAGCATTAGCATCGAGCGTTTGTCTCTGGATCAGCATCTCTTAAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGTCAGGGCGAGGt                                                                                                                                     <  6:168085/148‑1 (MQ=255)                 aCGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTgg                                                                                                                                                  <  7:91727/134‑1 (MQ=255)                 aCGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTgg                                                                                                                                                  >  8:91727/1‑134 (MQ=255)                 aCGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGa                                                                                                                                   <  3:194653/149‑1 (MQ=255)                   gcagcaACGCCGAACTCAAAAAGGCCCTTGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGa                                                                                                                                   <  3:23286/147‑1 (MQ=255)                     agcaACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCaa                                                                                                                               <  7:168261/149‑1 (MQ=255)                       caACGCCGAACTCAAAAAGGCCCTGGTCTGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAAtt                                                                                                                             <  1:3497/149‑1 (MQ=255)                       caACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAgg                                                                                                                                      <  7:91555/140‑1 (MQ=255)                       caACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAgg                                                                                                                                      >  8:91555/1‑140 (MQ=255)                         aCGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAAtttt                                                                                                                           <  4:41192/149‑1 (MQ=255)                             cGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGGGGGGCAGGGCGCGGTGATCAATTTTGCCg                                                                                                                       >  8:138111/1‑149 (MQ=255)                                  tCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGc                                                                                                                  >  1:6294/1‑149 (MQ=255)                                  tCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGc                                                                                                                  >  5:241447/1‑149 (MQ=255)                                    aaaaaGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTgg                                                                                                                    <  1:232216/145‑1 (MQ=255)                                    aaaaaGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAAATTTGACGTGGGCGt                                                                                                                >  1:36209/1‑149 (MQ=255)                                          gCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTgaccga                                                                                                          <  3:191125/149‑1 (MQ=255)                                            ccTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTgaccgac                                                                                                         >  7:237075/1‑148 (MQ=255)                                                gTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTgaccgac                                                                                                         >  4:272725/1‑144 (MQ=255)                                                   aGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGa                                                                                                 <  7:222964/149‑1 (MQ=255)                                                    gTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAg                                                                                                >  4:299347/1‑149 (MQ=255)                                                     tACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGTGCGTGACCGACCAGATCGAGc                                                                                               <  6:293760/149‑1 (MQ=255)                                                     tACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAg                                                                                                >  5:287875/1‑148 (MQ=255)                                                     tACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAg                                                                                                <  6:287875/148‑1 (MQ=255)                                                       ccGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTAGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCAc                                                                                             >  1:44396/1‑149 (MQ=255)                                                       ccGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTAGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGAAc                                                                                             >  5:175074/1‑149 (MQ=255)                                                         ggtggtAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACgg                                                                                           <  7:235173/149‑1 (MQ=255)                                                           tggtAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGcc                                                                                         <  3:299347/149‑1 (MQ=255)                                                             gtAGCATGAGGATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGccc                                                                                        <  7:138111/148‑1 (MQ=255)                                                                  aTGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTcc                                                                                  >  1:177888/1‑149 (MQ=255)                                                                    gAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTgaccgac                                                                                                         <  8:13464/124‑1 (MQ=255)                                                                    gAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTgaccgac                                                                                                         >  7:13464/1‑124 (MQ=255)                                                                      gCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTgg                                                                              <  6:241447/149‑1 (MQ=255)                                                                       cATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGt                                                                             <  2:177888/149‑1 (MQ=255)                                                                            aGCTTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTTATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAgg                                                                        <  8:237075/149‑1 (MQ=255)                                                                            aGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGTGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAgg                                                                        <  5:167256/149‑1 (MQ=255)                                                                                  tgtCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTAGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCATATCGAGCACGGCCCGTTTCCGTGGTCGAGggtcag                                                                   <  6:175074/148‑1 (MQ=255)                                                                                  tgtCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTAGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGggtcag                                                                   <  2:44396/148‑1 (MQ=255)                                                                                      gCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGt                                                                                <  4:259846/131‑1 (MQ=255)                                                                                      gCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGt                                                                                >  3:259846/1‑131 (MQ=255)                                                                                      gCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCAc                                                              <  3:90962/149‑1 (MQ=255)                                                                                               gcaTCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGt                                                                             >  5:38586/1‑125 (MQ=255)                                                                                               gcaTCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGt                                                                             <  6:38586/125‑1 (MQ=255)                                                                                                    tctTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCAGATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGcc                                                 >  1:69006/1‑149 (MQ=255)                                                                                                    tctTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTgg                                                                              <  8:54975/119‑1 (MQ=255)                                                                                                    tctTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTgg                                                                              >  7:54975/1‑119 (MQ=255)                                                                                                     ctTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTGTCCGTGGTCGa                                                                          >  6:96147/1‑122 (MQ=255)                                                                                                      ttCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCg                                                <  3:272725/147‑1 (MQ=255)                                                                                                           cagcaCTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGCCGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATgg                                         >  3:226692/1‑149 (MQ=255)                                                                                                            agcaCTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGc                                        >  7:67077/1‑149 (MQ=255)                                                                                                                             gCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGttt                                                                                    <  6:256653/88‑1 (MQ=255)                                                                                                                                 aaGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCt                                                       >  6:228788/1‑113 (MQ=255)                                                                                                                                 aaGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCt                                                       <  5:228788/113‑1 (MQ=255)                                                                                                                                 aaGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTg                     >  3:187183/1‑147 (MQ=255)                                                                                                                                     cTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTGCAGAAg               >  4:47439/1‑149 (MQ=255)                                                                                                                                        gcgACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTGCAGAAGGAt            <  2:6294/149‑1 (MQ=255)                                                                                                                                             aTCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCa                                   <  2:2586/121‑1 (MQ=255)                                                                                                                                             aTCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCa                                   >  1:2586/1‑121 (MQ=255)                                                                                                                                                   tgtgGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTGCAGAAGGATGACGCCGTTg  >  1:302885/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                  TGGACAACGCCCTTGCACGCAGCAACGCCGAACTCAAAAAGGCCCTGGTCAGTACCGGTGGTAGCATGAGCATCGAGCGTGTGTCGCTGGAGCAGCATCTCTTCAGCAGCACTGCCCAGTACCGGCTCAAGGCTCGCGACATCA‑ATGTGGGGCAGGGCGAGGTGATCAATTTTGACGTGGGCGTGACCGACCAGATCGAGCACGGCCCGTTTCCGTGGTCGAGGGTCAGGTCACTGAAGCTGATGCCGGTAATGGCGGTCAGCAACAGCACGCTGCAGAAGGATGACGCCGTTG  >  NC_002947/1407648‑1407941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑