Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,414,211 G→A 9.5% S86S (AGC→AGT)  PP_1236 ← glycine cleavage system transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,414,2110GA 9.5% 114.9 / 2.4 42S86S (AGC→AGT) PP_1236glycine cleavage system transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base A (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGCCAGGTAGGTGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGGCGCGAGGTG  >  NC_002947/1414063‑1414352
                                                                                                                                                    |                                                                                                                                             
gcgcCAGGTAGGTGTGGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCcgcg                                                                                                                                               >  2:6462/1‑149 (MQ=255)
   ccAGGTAGGTGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTg                                                                                                                                            <  1:280285/149‑1 (MQ=255)
    cAGGTAGGTGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCACTGc                                                                                                                                           >  3:278630/1‑149 (MQ=255)
     aggtaggtGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCg                                                                                                                                          >  7:227885/1‑149 (MQ=255)
       gtaggtGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCACTGCGGa                                                                                                                                        <  4:278630/149‑1 (MQ=255)
        taggtGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGAc                                                                                                                                       <  6:226177/149‑1 (MQ=255)
          ggtGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCAGCGCGGACCa                                                                                                                                     <  6:20207/149‑1 (MQ=255)
                gCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGa                                                                                                                               <  1:199606/149‑1 (MQ=255)
                gCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGa                                                                                                                               <  5:35392/149‑1 (MQ=255)
                  aGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAgg                                                                                                                             <  5:208470/149‑1 (MQ=255)
                  aGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAgg                                                                                                                             >  1:219212/1‑149 (MQ=255)
                     tCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGt                                                                                                                           >  8:167183/1‑148 (MQ=255)
                      cATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCAAGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTACTGGTCCGCGCGTCGGACGACTTCGAGGGt                                                                                                                           >  8:292405/1‑147 (MQ=255)
                        tGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACTGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACTTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAgg                                                                                                                       <  6:59934/149‑1 (MQ=255)
                                           gtggtCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGc                                                                                                    <  7:167183/149‑1 (MQ=255)
                                             ggtCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCt                                                                                                  >  7:105405/1‑149 (MQ=255)
                                              gtCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGa                                                                                                                               >  2:137189/1‑119 (MQ=255)
                                              gtCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGa                                                                                                                               <  1:137189/119‑1 (MQ=255)
                                                   ggAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAgg                                                                                            >  1:148018/1‑149 (MQ=255)
                                                   ggAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCAGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAgg                                                                                            >  3:298354/1‑149 (MQ=255)
                                                    taagaaCTGGCACAGCTCGTTGATTATGTCCGGGCGGTTGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCCGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAggg                                                                                           <  4:298354/148‑1 (MQ=255)
                                                     aagaaCTGGCACAGCTGGTTGATGATGTCCGGGGGGTTGGCAGCGCTCACGTCGGCAACATACGCACGGGCGTGGGGGCGCACTTCCTGGGCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAggg                                                                                           <  2:148018/148‑1 (MQ=255)
                                                     aagaaCTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGt                                                                                          <  8:227885/149‑1 (MQ=255)
                                                             gCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCga                                                                                  <  6:182250/149‑1 (MQ=255)
                                                                  gCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGCATTCGAGACGt                                                                             >  7:230898/1‑149 (MQ=255)
                                                                   cTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTg                                                                            <  2:219212/149‑1 (MQ=255)
                                                                          atgatgTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTc                                                                                                                          <  7:78661/96‑1 (MQ=255)
                                                                            gatgTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGt                                                                   >  5:114969/1‑149 (MQ=255)
                                                                                  cGGGCGTGAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGc                                                             <  4:207806/149‑1 (MQ=255)
                                                                                    ggCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGt                                                                   >  2:88630/1‑141 (MQ=255)
                                                                                    ggCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGt                                                                   <  1:88630/141‑1 (MQ=255)
                                                                                           ggCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAgg                                                                       >  8:215665/1‑130 (MQ=255)
                                                                                            gCAGCGCTCAGGTTGGCCACATCCGGACGGGCCTGCGGGCGCACTTCGTGGGCCGCGCTGCGCACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCAc                                                   <  8:105405/149‑1 (MQ=255)
                                                                                              aGCGCTCACGTTGGCCACATTCGGTTGGGCCTGCGGGGGCACTTCCTTGTCCGCGCTGGGGACCACGTCGTGGGTCAGGCCGTGCTTCTTGCCGATGCCTGGCAGGTTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCAccg                                                 <  8:230898/149‑2 (MQ=255)
                                                                                              aGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCt                                                 <  1:6462/149‑1 (MQ=255)
                                                                                                             cacaTACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGgcgc                                  >  2:179629/1‑149 (MQ=255)
                                                                                                                                      aCTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGgcg         >  5:181268/1‑149 (MQ=255)
                                                                                                                                        ttCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGgcgc        >  7:130671/1‑148 (MQ=255)
                                                                                                                                        ttCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGgcgc        >  2:61564/1‑148 (MQ=255)
                                                                                                                                        ttCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGgcgc        >  5:296888/1‑148 (MQ=255)
                                                                                                                                          cgtGGTCCGCAGCGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGGCGCGAg     >  1:321027/3‑149 (MQ=255)
                                                                                                                                               tCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGc                         <  1:158431/124‑1 (MQ=255)
                                                                                                                                               tCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGc                         >  2:158431/1‑124 (MQ=255)
                                                                                                                                                    gcTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGGCGCGAGGTg  >  6:58003/1‑142 (MQ=255)
                                                                                                                                                    gcTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGGCGCGAGGTg  <  5:58003/142‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCGCCAGGTAGGTGTCGCAGGTCATGGCTTCCAGCTCGACGCGGTGGTCAAGGAAGAACTGGCACAGCTCGTTGATGATGTCCGGGCGGTAGGCAGCGCTCACGTAGGCCACATACGGAAGGGCCTGCGGGCGCACTTCCTGGTCCGCGCTGCGGACCACGTCGAGGGTCAGGCCGTGCTTCTTGCCCAAGCCTGGCAGGGTGGATTCGAGACGTGCCAGGGCGTCCCAGCTGCCGCCCACCTGCAGCACCAGGGCGCTGGTCTCGCCGTGGCGGCTCAGGCGCGAGGTG  >  NC_002947/1414063‑1414352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑