Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,446,831 C→T 11.1% A310V (GCG→GTG)  PP_1264 → fusaric acid resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,446,8310CT11.1% 144.0 / 8.0 54A310V (GCG→GTG) PP_1264fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base T (2/4);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CCGCTTCAATGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCT  >  NC_002947/1446688‑1446974
                                                                                                                                                |                                                                                                                                               
ccGCTTCAATGCCTTGCACCGCTTGCTGAAGCGCATGCGTGCCCGTGGCCCGCTCCAAATTGTAAGCGAAATAGAAACGGGCCTAAATACCCAAGTGGAGTTGCAGCACCCCTATGTGGG‑CCGGGCGTTGACTGACGCCCAtccgctgc                                                                                                                                            >  2:296304/1‑149 (MQ=255)
  gCTTCAATGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGAtgcgctgcgc                                                                                                                                          >  4:111687/1‑149 (MQ=255)
       aaTGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAc                                                                                                                                     <  1:296304/149‑1 (MQ=255)
       aaTGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAc                                                                                                                                     >  8:79287/1‑149 (MQ=255)
        aTGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGAcc                                                                                                                                    >  7:57398/1‑149 (MQ=255)
        aTGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAcc                                                                                                                                    <  7:301061/149‑1 (MQ=255)
        aTGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAcc                                                                                                                                    >  7:216067/1‑149 (MQ=255)
             ttGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTg                                                                                                                                       >  1:220085/1‑141 (MQ=255)
             ttGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTg                                                                                                                                       <  2:220085/141‑1 (MQ=255)
               gCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGa                                                                                                                               <  8:13581/147‑1 (MQ=255)
               gCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGc                                                                                                                             >  6:26631/1‑149 (MQ=255)
                         cTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTa                                                                                                                   >  5:151916/1‑149 (MQ=255)
                            gAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaa                                                                                                                <  8:11265/149‑1 (MQ=255)
                                gcCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaa                                                                                                            <  4:309059/149‑1 (MQ=255)
                                 ccTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaag                                                                                                           <  2:98952/149‑1 (MQ=255)
                                       tGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTg                                                                                                      >  4:69355/1‑148 (MQ=255)
                                       tGCCCGTGGACCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCGCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGc                                                                                                     <  6:254425/149‑1 (MQ=255)
                                            gTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaag                                                                                                           <  3:113930/138‑1 (MQ=255)
                                            gTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaag                                                                                                           >  4:113930/1‑138 (MQ=255)
                                                 ccGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGt                                                                                           >  2:241194/1‑149 (MQ=255)
                                                    cTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCg                                                                                        >  7:259148/1‑149 (MQ=255)
                                                    cTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCg                                                                                        >  5:39738/1‑149 (MQ=255)
                                                      gCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggg                                                                                      <  3:69355/149‑1 (MQ=255)
                                                       cAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggg                                                                                      <  5:26631/148‑1 (MQ=255)
                                                              tCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTTCAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTgcgg                                                                              <  1:60061/149‑1 (MQ=255)
                                                               cAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggg                                                                                      <  8:102558/140‑1 (MQ=255)
                                                               cAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggg                                                                                      >  7:102558/1‑140 (MQ=255)
                                                                 gcgcTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTgcgggcg                                                                           >  5:156299/1‑149 (MQ=255)
                                                                     tATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAg                                                                        >  5:315036/1‑148 (MQ=255)
                                                                       tCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTAc                                                                     <  3:111687/149‑1 (MQ=255)
                                                                       tCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTAc                                                                     <  6:156299/149‑1 (MQ=255)
                                                                           aCCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGGACCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGCCCCAGGTGCGGGGGCTCCGGGCGGAGTACATg                                                                  >  1:68213/1‑149 (MQ=255)
                                                                           aCCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTgg                                                                 <  8:57398/149‑1 (MQ=255)
                                                                            ccGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGACGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTgg                                                                 >  8:72337/1‑148 (MQ=255)
                                                                                       tACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCa                                                     <  5:207453/149‑1 (MQ=255)
                                                                                          ccTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggggg                                                                                    <  8:16944/115‑1 (MQ=255)
                                                                                          ccTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCggggg                                                                                    >  7:16944/1‑115 (MQ=255)
                                                                                          ccTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAg                                                                        <  4:273636/127‑1 (MQ=255)
                                                                                              gtggAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATc                                              <  6:315036/149‑1 (MQ=255)
                                                                                                ggAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATcc                                             >  1:314854/1‑148 (MQ=255)
                                                                                                  aGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGTGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGAc                                          <  6:27470/149‑1 (MQ=255)
                                                                                                   gTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaag                                                                                                           >  1:26229/1‑83 (MQ=255)
                                                                                                   gTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACaaggaag                                                                                                           <  2:26229/83‑1 (MQ=255)
                                                                                                              ccTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTc                              <  8:216067/149‑1 (MQ=255)
                                                                                                               cTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTcc                             <  1:241194/149‑1 (MQ=255)
                                                                                                               cTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTcc                             <  6:151916/149‑1 (MQ=255)
                                                                                                                       g‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACcgcg                     >  6:175968/1‑149 (MQ=255)
                                                                                                                       g‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACcgcg                     >  8:213046/1‑149 (MQ=255)
                                                                                                                         ccGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGt                    <  7:79287/149‑1 (MQ=255)
                                                                                                                           gggCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTc                  <  5:175968/149‑1 (MQ=255)
                                                                                                                                 tgactgacGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAgctg            >  6:262089/1‑149 (MQ=255)
                                                                                                                                     tgacGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGctgctg         >  3:224193/1‑148 (MQ=255)
                                                                                                                                       acGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATGCCGCGTTCGAGCTGCTGTAc      >  8:263935/1‑149 (MQ=255)
                                                                                                                                           cGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCt  >  7:145970/1‑149 (MQ=255)
                                                                                                                                           cGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCt  >  7:145968/1‑149 (MQ=255)
                                                                                                                                           cGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCt  >  6:12709/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCGCTTCAATGCCTTGCACCGCTTGCTGGAGCGCCTGCGTGCCCGTGGCCCGCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTATGTGGG‑CCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGACCCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCT  >  NC_002947/1446688‑1446974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑