Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,465,581 C→T 100% A13T (GCC→ACC)  algX ← alginate biosynthesis protein AlgX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,465,5810CT100.0% 172.6 / NA 53A13T (GCC→ACC) algXalginate biosynthesis protein AlgX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTGGTGAAGTTTTGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGCGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCGTCCTGGCGCTCGCC  >  NC_002947/1465437‑1465726
                                                                                                                                                |                                                                                                                                                   
gCGTGGTGAAGTTTTGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGAc                                                                                                                                                 <  6:226725/149‑1 (MQ=255)
         aGTTTTGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCcag                                                                                                                                         >  5:254778/1‑148 (MQ=255)
           ttttGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCcagcag                                                                                                                                      >  5:272180/1‑149 (MQ=255)
            tttGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAgg                                                                                                                                              <  6:15321/140‑1 (MQ=255)
            tttGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAgg                                                                                                                                              >  5:15321/1‑140 (MQ=255)
              tGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGtt                                                                                                                                    <  1:154331/148‑1 (MQ=255)
                  gATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTtcat                                                                                                                                >  2:116193/1‑148 (MQ=255)
                      gCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAg                                                                                                                             <  1:276303/147‑1 (MQ=255)
                      gCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAg                                                                                                                             >  2:276303/1‑147 (MQ=255)
                             gtgtAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGt                                                                                                                   >  6:320228/1‑149 (MQ=255)
                                  gCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATgg                                                                                                              >  6:257953/1‑149 (MQ=255)
                                         gCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGc                                                                                                       >  4:130117/1‑149 (MQ=255)
                                            tCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTc                                                                                                    >  8:246876/1‑149 (MQ=255)
                                                      cAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGt                                                                                                                                     <  5:300687/107‑1 (MQ=255)
                                                      cAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGt                                                                                                                                     >  6:300687/1‑107 (MQ=255)
                                                            acaACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CAtt                                                                                     >  7:279679/1‑147 (MQ=255)
                                                              aaCTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGt                                                                                                                                     <  4:17004/99‑1 (MQ=255)
                                                              aaCTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGt                                                                                                                                     >  3:17004/1‑99 (MQ=255)
                                                                   gcagcaGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTGCTGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTg                                                                             <  1:64558/149‑1 (MQ=255)
                                                                   gcagcaGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTgg                                                                            <  6:272180/149‑1 (MQ=255)
                                                                   gcagcaGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTg                                                                             <  5:320228/148‑1 (MQ=255)
                                                                        agGGATCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTc                                                                       >  7:212876/1‑149 (MQ=255)
                                                                           gCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGtg‑tg                                                                                                                       >  2:251311/1‑99 (MQ=255)
                                                                           gCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGtg‑tg                                                                                                                       <  1:251311/99‑1 (MQ=255)
                                                                             tCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCg                                                                  >  3:256266/1‑149 (MQ=255)
                                                                               ggCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTc                                                                               <  7:89068/134‑1 (MQ=255)
                                                                               ggCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TCGAGTCATCGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTc                                                                               >  8:89068/1‑134 (MQ=255)
                                                                                gcgcTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGt                                                               >  2:302047/1‑149 (MQ=255)
                                                                                  gcTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTc                                                              >  2:222268/1‑148 (MQ=255)
                                                                                      aaGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGCT‑CATTTCGGTCTGGCGTTCGACCGGGTCGAt                                                           >  2:247551/1‑147 (MQ=255)
                                                                                       acctCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTGCGACCGGGTCGATCAg                                                        <  7:168458/146‑1 (MQ=255)
                                                                                        gggcGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGc                                                       <  5:213437/146‑1 (MQ=255)
                                                                                            ggggCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATccg                                                        >  8:168458/1‑142 (MQ=255)
                                                                                               gCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGAcc                                                                   >  2:184712/1‑130 (MQ=255)
                                                                                               gCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGAcc                                                                   <  1:184712/130‑1 (MQ=255)
                                                                                               gCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCt                                                >  8:129346/1‑149 (MQ=255)
                                                                                               gCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCt                                                >  5:92136/1‑149 (MQ=255)
                                                                                                  ttGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAAt                                                                                              <  4:73684/101‑1 (MQ=255)
                                                                                                  ttGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAAt                                                                                              >  3:73684/1‑101 (MQ=255)
                                                                                                      cGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATTGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTctgctg                                         >  1:252326/1‑149 (MQ=255)
                                                                                                        tCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGc                                       <  7:246876/149‑1 (MQ=255)
                                                                                                        tCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGc                                       <  1:116193/149‑1 (MQ=255)
                                                                                                         cTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGATCCATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGc                                       <  1:247551/149‑1 (MQ=255)
                                                                                                          ttCGGCGCGCCCTCACGGGCTGAGTTCCAGAAGGGAGGTTGTCAGGCCCAGAAGGTTCATCAGGTG‑TTGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGCCCgg                                                                 <  2:226353/121‑1 (MQ=255)
                                                                                                                        ccTGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAg                       <  7:129346/149‑1 (MQ=255)
                                                                                                                          tGGCTGATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGa                     >  4:266495/1‑149 (MQ=255)
                                                                                                                               gATTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTTCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCg                <  6:254778/149‑1 (MQ=255)
                                                                                                                                aTTGCCAGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTc                 <  3:130117/147‑1 (MQ=255)
                                                                                                                                    ccAGACGGGCGGTGCACAGGCCCAGCAGTTTCATCCGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCATCAGGTCGAGGATTTCGTCCTg           <  8:279679/149‑1 (MQ=255)
                                                                                                                                      aGAAGGGCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCGTCCTGgc         <  4:256266/149‑1 (MQ=255)
                                                                                                                                        taGGGCGGTGGAGAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCTACGGGGTCGATCAGGACAGCCTTctgctg                                         <  7:98966/114‑1 (MQ=255)
                                                                                                                                          gggCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCTTAAAAGCAGGTCGAGGATTTcc                >  8:68271/1‑137 (MQ=255)
                                                                                                                                          gggCGGTGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGGCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCg                <  7:68271/138‑1 (MQ=255)
                                                                                                                                             cggtggACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCtt                                                                                                 >  7:276611/1‑55 (MQ=255)
                                                                                                                                             cggtggACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCtt                                                                                                 <  8:276611/55‑1 (MQ=255)
                                                                                                                                             cggtggACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCGTCCTGGCGCTCGcc  <  6:92136/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCGTGGTGAAGTTTTGCTGATAGCGGGTGGTGTAGCGGCTTGCATCGTGCGCTTCAGGGCACAACTGGCAGCAGGGCTCGGCGCTGAAGGTCGGGGCCTTGACGTCTTCGGCGCGCACGCCCTGGCTGATTGCCAGAAGGGCGGCGGACAGGCCCAGCAGTTTCATCAGGTG‑TGGAGTCATGGTCTGGGCTTCCTTAATCGAT‑CATTTCGGTCTGGCGTTCGACCGGGTCGATCAGCACAGCCTTCTGCTGGCGTACCAGCAGGTCGAGGATTTCGTCCTGGCGCTCGCC  >  NC_002947/1465437‑1465726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑