Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,520,412 T→C 8.3% L251P (CTG→CCG)  mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,520,4120TC 8.3% 127.2 / 2.8 48L251P (CTG→CCG) mraYphospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

GTGATGGTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCGTGGT  >  NC_002947/1520276‑1520556
                                                                                                                                        |                                                                                                                                                
gTGATGGTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCtgtg                                                                                                                                      <  8:192949/149‑1 (MQ=255)
  gATGGTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTATGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGt                                                                                                                                    >  7:279200/1‑149 (MQ=255)
    tGGTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGGCGGGTAACGTGAAGGTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTc                                                                                                                                  <  6:243514/149‑1 (MQ=255)
      gTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCaa                                                                                                                                <  6:105277/149‑1 (MQ=255)
      gTCGGCGGCGCGCTGGGCATCATCTCCTACATGTGGGGTAACGAGAACTTCGCCGAAGTCATCGAGAGCAGCTAGGTAACGGGCTGGGGCGAAGTGTTGGTGTGCTGGGGCGCGGTCTAAGTTGCAGAACTCGGCTTTCTGTGGTTcgg                                                                                                                                >  6:189505/1‑147 (MQ=255)
                                   ccTGTCGGGTAACGGGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGc                                                                                                    <  5:233124/148‑1 (MQ=255)
                                     tGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGACGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGAc                                                                                                 >  8:278737/1‑149 (MQ=255)
                                      gTCGGGTAACGTGAAGTTCGCCGAATACCTGCCGATCCCCTACGTGCCGGGCTGGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAacac                                                                                                                             >  3:27727/1‑120 (MQ=255)
                                      gTCGGGTAACGTGAAGTTCGCCGAATACCTGCCGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAacac                                                                                                                             <  4:27727/120‑1 (MQ=255)
                                       tCGGGTAACGTGAAGTTCTCCGAATACAGGCTGATCCCCTCCGTGCCGGGCTCGGTCGATATGATCGTGGTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGAc                                                                                                 <  8:279200/147‑1 (MQ=255)
                                                   aaGTTCGCCGAATACCTGCTGATCCCCTACGTTCCGTGCGCGGGCGAACTGATCGTGTTCTGCGGCGAGCTGATCGGTGCCGGCCTGGTCTTTCTGTGGTTCAACAATTATCCGGCGCAAGTGTTCATGGGCGACGTCTTCGCGCTgg                                                                                    <  4:159133/148‑1 (MQ=255)
                                                   aaGATCGCCGAATACCTCCCGATCCCCTACGTGCCGGGCTCGGGCGAACTGAGCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCGTGCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGTCGACGTCGGCGCGCTg                                                                                     <  6:206232/147‑1 (MQ=255)
                                                            gAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGgcgc                                                                          >  6:285203/1‑149 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAgt                                                                                                              <  7:21710/107‑1 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCCGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGgcgcgc                                                                                       <  7:146268/130‑1 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCCGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGgcgcgc                                                                                       <  7:142085/130‑1 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCCGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGgcgcgc                                                                                       >  8:142085/1‑130 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCCGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGgcgcgc                                                                                       >  8:146268/1‑130 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCATGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAgt                                                                                                              >  8:21710/1‑107 (MQ=255)
                                                                  ctgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGAGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCt                                                                    >  5:48426/1‑149 (MQ=255)
                                                                   tgctgATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGATCAACACTTATCCGGCGCAAGTGTTCATGGTCTACGTCGGCGCGCTGGCACTTGGCGCCGCGCTg                                                                   >  1:221145/1‑149 (MQ=255)
                                                                     cgatctcccTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTgg                                                                  <  7:167075/142‑1 (MQ=255)
                                                                        aTCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCAc                                                              >  3:7403/1‑149 (MQ=255)
                                                                          ccccTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGTGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCa                                                            >  1:36297/1‑149 (MQ=255)
                                                                           cccTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGAACGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGACACTGGGCGCCGCGCTGGGCACCAt                                                           >  1:244033/1‑149 (MQ=255)
                                                                            ccTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTgg                                                                  >  8:167075/1‑141 (MQ=255)
                                                                            ccTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGACACTGGGCGCCGCGCTGGGCACCATc                                                          <  2:244033/149‑1 (MQ=255)
                                                                             cTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCg                                                         >  8:188345/1‑149 (MQ=255)
                                                                               aCGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGcc                                                       >  3:221434/1‑149 (MQ=255)
                                                                                    ccGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCa                                                   <  5:285203/148‑1 (MQ=255)
                                                                                     cgggcgcgggcgAACTGATCGTGTTCTGCGGCGCGCTTATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATc                                                 <  6:48426/149‑1 (MQ=255)
                                                                                      gggCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCg                                                >  8:146613/1‑149 (MQ=255)
                                                                                            gggCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCg                                                      <  4:283020/137‑1 (MQ=255)
                                                                                            gggCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCg                                                      >  3:283020/1‑137 (MQ=255)
                                                                                                      aTCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATc                                  <  1:130136/147‑1 (MQ=255)
                                                                                                      aTCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGGTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGt                                >  1:244115/1‑149 (MQ=255)
                                                                                                       tcctgtTCTGCGGCGCGCTGATCGGTGCCTGCCTGGGCTTTCTGTGCTTCCACACTTATCCCGCGCAAGGGTGCATTGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTg                               <  5:189505/146‑1 (MQ=255)
                                                                                                            ttCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCGTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGtt                          >  2:217307/1‑149 (MQ=255)
                                                                                                                  ggcgCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTcatcat                    <  8:35637/149‑1 (MQ=255)
                                                                                                                  ggcgCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTcatcat                    >  4:298726/1‑149 (MQ=255)
                                                                                                                   gcgcgcTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATg                   >  5:274393/1‑149 (MQ=255)
                                                                                                                      cgcTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGggc                <  4:221434/149‑1 (MQ=255)
                                                                                                                               gTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTc       >  5:225786/1‑149 (MQ=255)
                                                                                                                                  ccGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCgt     <  6:225786/148‑1 (MQ=255)
                                                                                                                                   cGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCgtgg   <  7:278737/149‑1 (MQ=255)
                                                                                                                                   cGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCgtg    >  8:160016/1‑148 (MQ=255)
                                                                                                                                   cGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCgtg    <  7:160016/148‑1 (MQ=255)
                                                                                                                                    ggCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCgtggt  <  3:298726/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                
GTGATGGTCGGCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGTAACGTGAAGTTCGCCGAATACCTGCTGATCCCCTACGTGCCGGGCTCGGGCGAACTGATCGTGTTCTGCGGCGCGCTGATCGGTGCCGGCCTGGGCTTTCTGTGGTTCAACACTTATCCGGCGCAAGTGTTCATGGGCGACGTCGGCGCGCTGGCACTGGGCGCCGCGCTGGGCACCATCGCCGTCATCGTGCGCCAGGAAATCGTGCTGTTCATCATGGGCGGCATCTTCGTGGT  >  NC_002947/1520276‑1520556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑