Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,535,380	C→G	6.2%	A130G (GCG→GGG) ‡	argJ →	glutamate N‑acetyltransferase/amino acid acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,535,380	0	C	G	6.2%	203.9 / 3.5	65	A130G (GCG→GGG) ‡	argJ	glutamate N‑acetyltransferase/amino acid acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base G (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTACCGGTATCAG  >  NC_002947/1535232‑1535521                                                                                                                                                     |                                                                                                                                              aacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgc                                                                                                                                               >  5:64492/1‑149 (MQ=255) aacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgc                                                                                                                                               <  4:221972/149‑1 (MQ=255)     ccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTg                                                                                                                                           <  1:290868/149‑1 (MQ=255)     ccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTg                                                                                                                                           <  3:179212/149‑1 (MQ=255)      cGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGc                                                                                                                                          <  2:219225/149‑1 (MQ=255)       ggCACGGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGGCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCa                                                                                                                                         <  5:199185/149‑1 (MQ=255)       ggCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTg                                                                                                                                           >  6:57814/1‑147 (MQ=255)          aCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgcg                                                                                                                                              <  1:249244/141‑1 (MQ=255)          aCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGgcgcg                                                                                                                                              >  2:249244/1‑141 (MQ=255)            cGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGgccg                                                                                                                                    >  8:242527/1‑149 (MQ=255)              gcgcgcCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAGGGCGGCCTGCAGcgcgcc                                                                                                                                  >  1:88180/1‑149 (MQ=255)              gcgcgcCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAGGGCGGCCTGCAGcgcgcc                                                                                                                                  >  7:228180/1‑149 (MQ=255)                gcgcCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCt                                                                                                                                >  4:196120/1‑149 (MQ=255)                  gcCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCg                                                                                                                              <  7:242572/149‑1 (MQ=255)                    cAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGa                                                                                                                             >  7:110425/1‑148 (MQ=255)                       gCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTc                                                                                                                               >  2:24471/1‑143 (MQ=255)                       gCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTc                                                                                                                               <  1:24471/143‑1 (MQ=255)                       gCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAAc                                                                                                                         <  7:265067/149‑1 (MQ=255)                        ccTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAGGGCGGCCTGCAGCGCGCCTTCGatcttt                                                                                                                        <  8:228180/149‑6 (MQ=255)                        ccTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAGGGCGGCCTGCAGCGCGCCTTCGatcttt                                                                                                                        <  2:88180/149‑6 (MQ=255)                            gccgccgccGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTc                                                                                                                    <  7:242307/149‑1 (MQ=255)                            gccgccgccGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTc                                                                                                                    <  3:196120/149‑1 (MQ=255)                              cgccgccgAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCgg                                                                                                                  <  6:64492/149‑1 (MQ=255)                              cgccgccgAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCg                                                                                                                   >  6:279304/1‑148 (MQ=255)                                  gccgAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGaaaa                                                                                                              >  8:180717/1‑149 (MQ=255)                                             cTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTTGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCt                                                                                                    >  3:306189/1‑148 (MQ=255)                                                cgcCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGa                                                                                                  <  7:180717/147‑1 (MQ=255)                                                   cAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTc                                                                                                                    <  1:317413/126‑1 (MQ=255)                                                   cAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTc                                                                                                                    >  2:317413/1‑126 (MQ=255)                                                         ggCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCg                                                                                       <  8:110425/149‑1 (MQ=255)                                                                   gctggcGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGac                                                                             >  7:224439/1‑149 (MQ=255)                                                                                        gTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAgg                                                        >  5:632/1‑149 (MQ=255)                                                                                        gTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAgg                                                        <  7:242527/149‑1 (MQ=255)                                                                                        gTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAgg                                                        >  2:321006/1‑149 (MQ=255)                                                                                            tGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGc                                                     >  5:206890/1‑148 (MQ=255)                                                                                             gCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGc                                                     >  3:57560/1‑147 (MQ=255)                                                                                               cGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAgc                                                 <  5:57814/149‑1 (MQ=255)                                                                                               cGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAgc                                                 >  2:37465/1‑149 (MQ=255)                                                                                                     ccACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGTGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAg                                           >  4:237571/1‑149 (MQ=255)                                                                                                     ccACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAg                                           >  4:198612/1‑149 (MQ=255)                                                                                                      cACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAg                                           <  6:206890/148‑1 (MQ=255)                                                                                                      cACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGt                                          <  1:321006/149‑1 (MQ=255)                                                                                                       aCCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCt                                                                 <  2:77288/125‑1 (MQ=255)                                                                                                       aCCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCt                                                                 >  1:77288/1‑125 (MQ=255)                                                                                                       aCCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGtt                                         >  5:331366/1‑149 (MQ=255)                                                                                                         cGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCCCCGCCACCCTGCCCAAAGGGGCCAGCCGCCAGTTc                                        >  2:104468/1‑148 (MQ=255)                                                                                                         cGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTcc                                       <  3:198612/149‑1 (MQ=255)                                                                                                           gCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGcatc                                                                                  >  5:137828/1‑104 (MQ=255)                                                                                                           gCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGcatc                                                                                  <  6:137828/104‑1 (MQ=255)                                                                                                           gCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAg                                     >  3:52874/1‑149 (MQ=255)                                                                                                           gCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAg                                     >  3:180708/1‑149 (MQ=255)                                                                                                            cGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGc                                    <  4:57560/149‑1 (MQ=255)                                                                                                               gATTGGTGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCAc                                  >  2:314448/1‑148 (MQ=255)                                                                                                                    gCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGGCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGc                            <  8:224439/149‑1 (MQ=255)                                                                                                                      gAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTTGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCa                                                   <  2:287657/124‑1 (MQ=255)                                                                                                                      gAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCa                                                   >  1:287657/1‑124 (MQ=255)                                                                                                                          cGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGAcc                      <  6:632/149‑1 (MQ=255)                                                                                                                           gCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCg                     >  5:45962/1‑149 (MQ=255)                                                                                                                            cTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGt                    >  2:12413/1‑149 (MQ=255)                                                                                                                               ccGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCAc                 >  5:154470/1‑149 (MQ=255)                                                                                                                                     gAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTAc           >  5:268681/1‑149 (MQ=255)                                                                                                                                     gAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTAc           >  5:222479/1‑149 (MQ=255)                                                                                                                                     gAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTAc           >  2:211352/1‑149 (MQ=255)                                                                                                                                     gAAAAGATCGAAGGCGCGCTGCAGGCCGCCATCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCa                                      <  8:299120/122‑1 (MQ=255)                                                                                                                                     gAAAAGATCGAAGGCGCGCTGCAGGCCGCCATCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCa                                      >  7:299120/1‑122 (MQ=255)                                                                                                                                      aaaaGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTAc           <  4:306189/148‑1 (MQ=255)                                                                                                                                       aaaGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTACCg         <  6:222479/149‑1 (MQ=255)                                                                                                                                              gAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTACCGGTATCa   <  1:37465/148‑1 (MQ=255)                                                                                                                                              gAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTACCGGTATCAg  >  2:94678/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                              AACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCTGCAGGCCGCCCTCGACAACCTGTCGGAAAACAACTGGGCTGAGGCCGCTACCGGCATCATGACCACCGACACCCTGCCCAAAGGGGCCAGCCGCCAGTTCCAGCACGATGGCGTGACCGTCACCGTTACCGGTATCAG  >  NC_002947/1535232‑1535521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑