Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,541,275 C→T 5.8% A84T (GCC→ACC)  panE ← 2‑dehydropantoate 2‑reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,541,2750CT 5.8% 211.2 / 2.6 69A84T (GCC→ACC) panE2‑dehydropantoate 2‑reductase
Reads supporting (aligned to +/- strand):  ref base C (31/34);  new base T (2/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTGCAGCCGTTGCC  >  NC_002947/1541130‑1541421
                                                                                                                                                 |                                                                                                                                                  
aaGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCGATTGCCGGTGTAGc                                                                                                                                                 <  6:302169/149‑1 (MQ=255)
      ccTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCa                                                                                                                                             >  5:118106/1‑147 (MQ=255)
      ccTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCa                                                                                                                                             >  1:293466/1‑147 (MQ=255)
       cTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGTCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAg                                                                                                                                          <  4:33842/149‑1 (MQ=255)
             tGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTg                                                                                                                                    <  7:173000/149‑1 (MQ=255)
               cTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTg                                                                                                                                    >  4:56551/1‑147 (MQ=255)
                tGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCCGCACTTAGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAg                                                                                                                                 >  5:29024/1‑149 (MQ=255)
                     gCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGccac                                                                                                                            >  2:305095/1‑149 (MQ=255)
                       gAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGccacca                                                                                                                          <  7:128041/149‑1 (MQ=255)
                        aaGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACcag                                                                                                                         <  5:190288/149‑1 (MQ=255)
                        aaGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACcag                                                                                                                         >  6:219182/1‑149 (MQ=255)
                            tGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCagcagc                                                                                                                     <  8:274530/149‑1 (MQ=255)
                             gCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGcc                                                                                                                    >  4:84605/1‑149 (MQ=255)
                                gcgagcgTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGt                                                                                                                 <  2:293466/149‑1 (MQ=255)
                                    gcgTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGAt                                                                                                             <  4:68511/149‑1 (MQ=255)
                                    gcgTGAGGCACCAGGTCAGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTGCCCCGGCGATTGCCGGTGTAGCGCCa                                                                                                                                             >  3:195869/1‑117 (MQ=38)
                                    gcgTGAGGCACCAGGTCAGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTGCCCCGGCGATTGCCGGTGTAGCGCCa                                                                                                                                             <  4:195869/117‑1 (MQ=38)
                                         aGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCACCAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGc                                                                                                        <  6:47767/149‑1 (MQ=255)
                                         aGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGc                                                                                                        <  6:47763/149‑1 (MQ=255)
                                           gCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCt                                                                                                      >  3:47964/1‑149 (MQ=255)
                                             aCCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTg                                                                                                    >  3:272519/1‑149 (MQ=255)
                                              ccAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTgc                                                                                                   >  8:217833/1‑149 (MQ=255)
                                                aGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTgcgc                                                                                                 >  5:285816/1‑149 (MQ=255)
                                                aGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTACAGGCCACCAGCAGCCGGTGGATCGGGCCTTgcgc                                                                                                 <  6:236680/149‑1 (MQ=255)
                                                           ttCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTAGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGtt                                                                                       >  5:112676/1‑148 (MQ=255)
                                                             cATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGttt                                                                                      <  4:272519/147‑1 (MQ=255)
                                                             cATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGttt                                                                                      <  2:27204/147‑1 (MQ=255)
                                                              aTCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCgg                                                                                   >  6:165311/1‑149 (MQ=255)
                                                               tCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGc                                                                                  <  5:219182/149‑1 (MQ=255)
                                                                ccTGGCTGCCTAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGcc                                                                                 >  4:120434/1‑149 (MQ=255)
                                                                 cTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCg                                                                                <  3:233977/149‑1 (MQ=255)
                                                                       gCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATgg                                                                          >  1:260895/1‑149 (MQ=255)
                                                                             gCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCgg                                                                                                                  <  3:196156/103‑1 (MQ=255)
                                                                             gCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCgg                                                                                                                  >  4:196156/1‑103 (MQ=255)
                                                                               cATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAgcgc                                                                  >  6:301150/1‑149 (MQ=255)
                                                                                 ttCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGc                                                                                  <  8:72481/131‑1 (MQ=255)
                                                                                 ttCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGc                                                                                  >  7:72481/1‑131 (MQ=255)
                                                                                     gCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGtt                                                            >  8:237657/1‑149 (MQ=255)
                                                                                       aaCAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTg                                                          <  7:217833/149‑1 (MQ=255)
                                                                                       aaCAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTg                                                          <  6:285816/149‑1 (MQ=255)
                                                                                           gcagcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTcc                                                      >  6:33411/1‑149 (MQ=255)
                                                                                           gcagcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTcc                                                      <  6:118106/149‑1 (MQ=255)
                                                                                             agcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGTAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTg                                                    >  6:120074/1‑149 (MQ=255)
                                                                                              gcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGc                                                   >  5:232074/1‑149 (MQ=255)
                                                                                              gcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGc                                                   <  3:84605/149‑1 (MQ=255)
                                                                                               caCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCt                                                  <  1:305095/149‑1 (MQ=255)
                                                                                                  ttCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGa                                               >  5:322308/1‑149 (MQ=255)
                                                                                                            tcGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTc                                                       <  4:240050/131‑1 (MQ=255)
                                                                                                            tcGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTc                                                       >  3:240050/1‑131 (MQ=255)
                                                                                                            tcGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTc                                       <  4:47964/147‑1 (MQ=255)
                                                                                                              ggCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGc                                   >  2:135673/1‑149 (MQ=255)
                                                                                                              ggCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGc                                   >  5:304902/1‑149 (MQ=255)
                                                                                                                    ccGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCgg                             <  7:237657/149‑1 (MQ=255)
                                                                                                                         gcgcTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCctgc                        >  1:273174/1‑149 (MQ=255)
                                                                                                                          cgcTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCctgct                       <  5:165311/149‑1 (MQ=255)
                                                                                                                            cTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAgc                                                                    >  5:12752/1‑102 (MQ=255)
                                                                                                                            cTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAgc                                                                    <  6:12752/102‑1 (MQ=255)
                                                                                                                                 ccGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGc                <  3:120434/149‑1 (MQ=255)
                                                                                                                                 ccGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGc                <  6:112676/149‑1 (MQ=255)
                                                                                                                                    gCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTgg                                                         <  3:287488/105‑1 (MQ=255)
                                                                                                                                    gCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTgg                                                         >  4:287488/1‑105 (MQ=255)
                                                                                                                                     cAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGACGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTg             >  6:160801/1‑148 (MQ=255)
                                                                                                                                        tCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCt              <  7:230895/144‑1 (MQ=255)
                                                                                                                                        tCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCt              >  8:230895/1‑144 (MQ=255)
                                                                                                                                          gCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTgg                                                         <  3:294336/99‑1 (MQ=255)
                                                                                                                                          gCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTgg                                                         >  4:294336/1‑99 (MQ=255)
                                                                                                                                           ccGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTGCAGCCGt      <  5:301150/149‑1 (MQ=255)
                                                                                                                                              gggCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTGCAGCCGTTGc   <  1:135673/149‑1 (MQ=255)
                                                                                                                                               ggCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGTCCTGCTGATAGGCCTGCAGCCGTTGcc  <  5:33411/149‑1 (MQ=255)
                                                                                                                                                 cAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGcc                                                                                 <  7:228218/68‑1 (MQ=255)
                                                                                                                                                 cAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGcc                                                                                 >  8:228218/1‑68 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTGCAGCCGTTGCC  >  NC_002947/1541130‑1541421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑