| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,545,072 | T→C | 10.8% | D344G (GAC→GGC) | ampG ← | muropeptide permease AmpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,545,072 | 0 | T | C | 10.8% | 93.5 / 3.4 | 37 | D344G (GAC→GGC) | ampG | muropeptide permease AmpG |
| Reads supporting (aligned to +/- strand): ref base T (17/16); new base C (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
CCGGCAGAGGCTACGCCGCCGATGAACAGGATGGGCATGATGCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTTGATCGGGGTCAGCACGGGGGCCAGGCCGCGA > NC_002947/1544925‑1545215 | ccGGCAGAGGCTACGCCGCCGATGAACAGGATGGGCATGATGCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTc > 6:100591/1‑149 (MQ=255) gCTACGCCGCCGATGAACAGGATGGGCATGATGCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGaa < 3:188133/149‑1 (MQ=255) cgccgcCGATGAACAGGATGGGCATGATGCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAaca < 5:100591/147‑1 (MQ=255) atgatgCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGTACACGTTGGCCATCACCCCCATCACCgt < 1:188481/149‑1 (MQ=255) gatgCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCgtgt > 5:156065/1‑149 (MQ=255) tgCCTAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGTGTCATGACCAGGCCTAAGATCTTGCTGACGCTGGCAATCTGTTCCTTGGTGAAGCCCATGTCGatat < 2:199573/114‑1 (MQ=255) tgCCGAAGCGTACGATCAGCAGGCCGCAAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGatat > 1:199573/1‑114 (MQ=255) aGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATg < 3:104754/149‑1 (MQ=255) gCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGc > 2:315875/1‑149 (MQ=255) gTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGa > 5:290074/1‑149 (MQ=255) gATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAgg > 6:252397/1‑149 (MQ=255) gcagGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCgtg < 1:322528/128‑1 (MQ=255) gcagGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCgtg > 2:322528/1‑128 (MQ=255) agGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGCCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGAt > 4:24511/1‑149 (MQ=255) agGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCAGAAGATCTTGCTGACGCTGGCAATCTGGTCCTAGGTGAAGCCCATGCAGATAAAGAACACGATGGCCATCACCCCCATCACCGATACCGACATGAGATAGGTg > 4:218583/1‑144 (MQ=255) gccgccAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCa < 8:285258/149‑1 (MQ=255) gccAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATc > 7:350/1‑115 (MQ=255) gccAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATc < 8:350/115‑1 (MQ=255) gcgcCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCcagcagcag < 1:104748/149‑1 (MQ=255) gcCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCcagcagcag > 4:117450/1‑147 (MQ=255) cGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGCCTATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCaga < 3:218583/149‑1 (MQ=255) cGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGCCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCaga < 3:24511/149‑1 (MQ=255) aGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTg < 7:184525/149‑1 (MQ=255) gggTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATCCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTg > 1:47134/1‑148 (MQ=255) aCCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGt < 1:315875/148‑1 (MQ=255) cAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGc < 6:156065/149‑1 (MQ=255) aCGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCCTCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGtg > 7:211622/1‑135 (MQ=255) aCGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGtg < 8:211622/135‑1 (MQ=255) gCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAgg < 4:123310/97‑1 (MQ=255) gCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAgg > 3:123310/1‑97 (MQ=255) ggCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTCTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTAGAGCgggg > 1:302719/1‑149 (MQ=255) gCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTTGATCggg < 5:252397/147‑1 (MQ=255) ttGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTTGATCGGGGTCAGCACGGGGGc > 6:204850/1‑149 (MQ=255) aaGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTTGATCGGGGTCAGCACGGGGGCCAGGcc > 1:35202/1‑149 (MQ=255) gCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGtga > 2:164554/1‑110 (MQ=255) gCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGtga < 1:164554/110‑1 (MQ=255) ccATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCCATACGTGGCGATCAGGCCCAGCACAACCAGAGCCTGCCAGCGGAAGCGGGTCATGAAGTCGTTGATCGGGGTCAGCACGGGGGCCAGGCCGCGa > 5:206261/1‑149 (MQ=255) | CCGGCAGAGGCTACGCCGCCGATGAACAGGATGGGCATGATGCCGAAGCGTACGATCAGCAGGCCGCCAACCCCGGCGCCGACCAGGGTCATGACCAGGCCGAAGATCTTGCTGACGCTGGCAATCTGGTCCTTGGTGAAGCCCATGTCGATATAGAACACGTTGGCCATCACCCCCATCACCGTGTCCGACATGCGATAGGTGGCGATCAGGCCCAGCAGCAGCAGAGCCTGCCAGCGGTAGCGGGTGATGAAGTCGTTGATCGGGGTCAGCACGGGGGCCAGGCCGCGA > NC_002947/1544925‑1545215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |