Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,567,259 A→T 8.9% intergenic (+63/‑258) pcaR → / → pcaK pca regulon transcriptional regulator/4‑hydroxybenzoate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,567,2590AT 8.9% 146.5 / 2.9 53intergenic (+63/‑258)pcaR/pcaKpca regulon transcriptional regulator/4‑hydroxybenzoate transporter
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base T (3/2);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCACCC  >  NC_002947/1567111‑1567402
                                                                                                                                                    |                                                                                                                                                
gggTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATaaa                                                                                                                                                  >  4:81802/1‑149 (MQ=255)
    gACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGTTTATCGca                                                                                                                                              >  7:49481/1‑149 (MQ=255)
       tcGCAGTTAACTGGAGCAGCGGTTCTTTCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTTTTTGGTTGAGACCACAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGcaca                                                                                                                                            <  7:201263/147‑1 (MQ=255)
       ccGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGTTTATCGCACAg                                                                                                                                           <  8:49481/149‑1 (MQ=255)
       ccGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAg                                                                                                                                           >  7:194872/1‑149 (MQ=255)
        cGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAgt                                                                                                                                          <  1:61142/149‑1 (MQ=255)
         gCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAgtg                                                                                                                                         >  1:129418/1‑149 (MQ=255)
         gCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCACAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGcgca                                                                                                                                            >  8:201263/1‑146 (MQ=255)
            gTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCg                                                                                                                                      >  4:255719/1‑149 (MQ=255)
                    gAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGt                                                                                                                             <  2:158906/149‑1 (MQ=255)
                    gAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGt                                                                                                                             >  2:77340/1‑149 (MQ=255)
                         gcGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAAtt                                                                                                                        <  6:49523/149‑1 (MQ=255)
                           ggTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGc                                                                                                                      <  5:219631/149‑1 (MQ=255)
                            gTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCg                                                                                                                     >  1:101014/1‑149 (MQ=255)
                            gTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAACACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCg                                                                                                                     >  8:88976/1‑149 (MQ=255)
                             ttCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGt                                                                                                                    <  2:101014/149‑1 (MQ=255)
                                   ccGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACg                                                                                                                                                >  6:253677/1‑116 (MQ=255)
                                   ccGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACg                                                                                                                                                <  5:253677/116‑1 (MQ=255)
                                            cTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCt                                                                                                      >  4:191709/1‑148 (MQ=255)
                                               gCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGAtt                                                                                                  <  1:40328/149‑1 (MQ=255)
                                                cagccagcCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTa                                                                                                 <  1:22315/149‑1 (MQ=255)
                                                 agccagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTaa                                                                                                >  5:114397/1‑149 (MQ=255)
                                                  gccagccGGGACCTTTGCCACCAGTTCTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAAt                                                                                               <  8:9573/149‑1 (MQ=255)
                                                    cagccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATg                                                                                              >  3:221682/1‑148 (MQ=255)
                                                    cagccGGGACC‑TTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGtc                                                                                            >  7:104608/1‑149 (MQ=255)
                                                     agccGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGt                                                                                             >  3:265244/1‑148 (MQ=255)
                                                          ggACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCg                                                                                       <  5:316255/149‑1 (MQ=255)
                                                                         gttgttTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGc                                                                        <  8:194872/149‑1 (MQ=255)
                                                                            gttTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccga                                                                     >  7:13275/1‑149 (MQ=255)
                                                                               tGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgacc                                                                   <  3:191709/148‑1 (MQ=255)
                                                                                 gTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccgtt                                                                <  8:104608/149‑3 (MQ=255)
                                                                                 gTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccgt                                                                 <  8:13275/148‑2 (MQ=255)
                                                                                    gagaCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAATCGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccgttgc                                                              <  4:221682/148‑5 (MQ=255)
                                                                                    gagaCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgaccgttgc                                                              <  2:80169/148‑5 (MQ=255)
                                                                                     agaCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAg                                                            <  3:255719/149‑1 (MQ=255)
                                                                                       aCCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaa                                                          <  4:265244/149‑1 (MQ=255)
                                                                                         cGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaaa                                                         >  3:162480/1‑148 (MQ=255)
                                                                                          gCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGTTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGaaaaa                                                       <  1:77340/149‑1 (MQ=255)
                                                                                            aGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCGAGGACGGGTGAATTGAGTCACCTCTGGCGGCTGATTAAAGTCTCTCGCAACGCTCGGCTATCCCACCGAACAATaaaaa                                                       >  1:266899/1‑148 (MQ=255)
                                                                                                 gCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTa                                                  >  3:237600/1‑147 (MQ=255)
                                                                                                              ccGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCAt                                   >  5:83675/1‑149 (MQ=255)
                                                                                                              ccGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCAt                                   >  4:130698/1‑149 (MQ=255)
                                                                                                                   tCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATa                              <  7:88976/149‑1 (MQ=255)
                                                                                                                     aGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACt                            <  2:244739/149‑1 (MQ=255)
                                                                                                                      gAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCCTCACCTCGGCCCGCTGATTAAAGTCACTCGCAACGGTCGGCTAGCCGACCGAACAACAAAAACACAAGAGGCACTCACTCAGTAATACTg                           >  6:227229/1‑149 (MQ=255)
                                                                                                                       aaCAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGt                          >  2:34373/1‑149 (MQ=255)
                                                                                                                                aaCCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTTTCCCCTCgct                 >  4:243819/1‑149 (MQ=255)
                                                                                                                                aaCCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCgct                 >  3:221610/1‑149 (MQ=255)
                                                                                                                                    gTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgacc                                                                   >  3:212176/1‑95 (MQ=255)
                                                                                                                                    gTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGccgacc                                                                   <  4:212176/95‑1 (MQ=255)
                                                                                                                                          tgtgCGTTTATCGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCg       >  3:253383/1‑149 (MQ=255)
                                                                                                                                          tgtgCGTTTATCGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCACGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCg       >  5:219360/1‑149 (MQ=255)
                                                                                                                                              cGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCAcc   <  4:237600/149‑1 (MQ=255)
                                                                                                                                               gATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCAccc  >  2:110886/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGGTGACCCGCAGTGAACTGGAGCAGCGGTTCTTGCCGATTTTGCTGGCAGCCAGCCGGGACCTTTGCCACCAGTTGTTTGGTTGAGACCGCAGGGAGCACTTTGCGGCCCCGGATCAGAACAGGCAAAACCGTTTCCTGTGCGATAAACGCACAGTGTCG‑TGCCGGGTGAATTGCGTCACCTCGGCCGGCTGATTAATGTCTCTCGCAACGGTCGGCTAGCCGACCGAACAAGAAAAACACTAGAGGCACTCACTCATGAATACTGTCACCTCGCTGCTGCGGCCGCACCC  >  NC_002947/1567111‑1567402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑