| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,570,332 | C→T | 89.6% | G381G (GGC→GGT) | pcaF‑I → | beta‑ketoadipyl‑CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,570,332 | 0 | C | T | 89.6% | 143.2 / 7.9 | 59 | G381G (GGC→GGT) | pcaF‑I | beta‑ketoadipyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (22/30); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01 | |||||||||||
TGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGC > NC_002947/1570187‑1570477 | tgcGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTg > 4:28596/1‑149 (MQ=255) cGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTgg < 7:253549/148‑1 (MQ=255) gTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGc > 1:116038/1‑148 (MQ=255) ggggtgGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCAtgt < 8:141090/145‑1 (MQ=255) ggTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCAtgt < 1:174326/148‑1 (MQ=255) gcCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGAACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAg < 1:58730/149‑1 (MQ=255) ccAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTc < 2:99135/149‑1 (MQ=255) aaaCCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGt > 2:251082/1‑149 (MQ=255) aaCCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGtt < 4:317522/149‑1 (MQ=255) cccTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGTGTTgg > 3:81914/1‑149 (MQ=255) cccTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTgg < 1:20951/149‑1 (MQ=255) ccTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGc < 7:63000/149‑1 (MQ=255) cTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGc > 6:123177/1‑148 (MQ=255) tAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCa > 5:131743/1‑149 (MQ=255) cGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGTGTTGGCCATCGAGc < 4:81914/149‑1 (MQ=255) ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt < 3:144839/149‑1 (MQ=255) ccATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt < 3:28596/149‑1 (MQ=255) aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt > 3:273513/1‑148 (MQ=255) aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGttt > 2:103151/1‑148 (MQ=255) aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt > 5:317663/1‑147 (MQ=255) aTTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGtt < 6:317663/147‑1 (MQ=255) ccTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGCCCTg > 2:12246/1‑149 (MQ=255) tGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTa > 5:64230/1‑149 (MQ=255) ggCAACCCCCTGGGCATGTGCGGGGAACGCCTGGTACTGACTGC‑GTGGCACATGGTGTTGATTAGTGGCGTGCGCAAGGGTCTGGCGACAAGGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTAcc < 1:251082/149‑1 (MQ=255) ccACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGCGGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCg > 1:64735/1‑149 (MQ=255) ccACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCAtgt > 8:176945/1‑92 (MQ=255) ccACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCAtgt < 7:176945/92‑1 (MQ=255) ccccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCctc > 7:72902/1‑149 (MQ=255) ccccTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACAGGCctc > 8:288761/1‑149 (MQ=255) aTGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCggg > 3:186828/1‑148 (MQ=255) gCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGc < 4:186828/149‑1 (MQ=255) tGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCggg > 2:277555/1‑113 (MQ=255) tGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCggg < 1:277555/113‑1 (MQ=255) cACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTc > 5:17007/1‑149 (MQ=255) cTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACa < 2:303659/149‑1 (MQ=255) ctgactgC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCgg < 7:252110/126‑1 (MQ=255) ctgactgC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGTTCCAACAGGGAt < 4:273513/148‑1 (MQ=255) ctgactgC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGAt < 5:123177/148‑1 (MQ=255) ctgC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACa < 4:34961/139‑1 (MQ=255) ctgC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACa > 3:34961/1‑139 (MQ=255) gTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGa > 4:37561/1‑139 (MQ=255) gTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGa < 3:37561/139‑1 (MQ=255) cACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCAc < 8:72902/148‑1 (MQ=255) cAGCTGGAGAAGAGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCtgt > 5:5159/1‑149 (MQ=255) tgaagaGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCg < 1:5179/148‑1 (MQ=255) gaagaGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCgg < 6:131743/149‑1 (MQ=255) aagaGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGa < 7:67482/123‑1 (MQ=255) aagaGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGa > 8:67482/1‑123 (MQ=255) aagaGTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCg > 2:5179/1‑147 (MQ=255) agagTGGCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGc < 6:64230/149‑1 (MQ=255) gCGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTcccc < 6:17007/149‑1 (MQ=255) gCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTcccc > 4:44291/1‑149 (MQ=255) cGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTgg < 6:100954/134‑1 (MQ=255) cGGTCGCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTgg > 5:100954/1‑134 (MQ=255) gCAAGGGTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGaa < 2:64735/148‑1 (MQ=255) cAAGGGGCGGGCGACCATGTGTGTGGGTGGCGCCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTCACCTGTACCGGCCTCTTCGCGGGTACGCCCGCCCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAaga < 2:116038/149‑1 (MQ=255) aaGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGc > 4:161436/1‑135 (MQ=255) aaGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGc < 3:161436/135‑1 (MQ=255) gggTCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGc < 1:12246/149‑1 (MQ=255) | TGCGTGAGCTGGGTGTGGCTGACGATGCGCCCCAGGTAAACCCTAATGGCGGTGCCATTGCCCTGGGCCACCCCCTGGGCATGAGCGGTGCACGCCTGGTACTGACTGC‑GTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCGGGGAAGCCCGCTCCAACAGGGATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGC > NC_002947/1570187‑1570477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |