Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,018 G→A 9.1% P155S (CCG→TCG)  galP‑II ← porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,0180GA 9.1% 119.4 / 2.5 44P155S (CCG→TCG) galP‑IIporin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base A (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

TGAAGGCGGCTTTACCGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGGGCGGCCG  >  NC_002947/1575880‑1576148
                                                                                                                                          |                                                                                                                                  
tGAAGGCGGCTTTACCGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCgg                                                                                                                          >  3:244087/1‑149 (MQ=255)
  aaGGCGGCTTTACCGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGc                                                                                                                         >  4:258116/1‑148 (MQ=255)
    ggcggcTTTACCGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCAt                                                                                                                      >  2:10941/1‑149 (MQ=255)
             agcGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGaa                                                                                                              <  5:219278/146‑1 (MQ=255)
             aCCGTTGAGCGTCATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAAc                                                                                                             >  1:112121/1‑149 (MQ=255)
                gTTGAGCGACATGCCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGc                                                                                                           >  1:231323/1‑148 (MQ=255)
                gTTGAGCGACATGCCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGc                                                                                                           <  2:231323/148‑1 (MQ=255)
                    aGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTAAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGAt                                                                                                      >  2:674/1‑149 (MQ=255)
                      agACATGTCTTCCTTGCTTGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGTAATGTTTGCGGCAGTGAGCGTCCATCGTCCGAACGCAGTATTg                                                                                                    <  2:118265/148‑1 (MQ=21)
                       gACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTgg                                                                                                   <  6:101015/149‑1 (MQ=255)
                        aCATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGc                                                                                                  <  2:56792/149‑1 (MQ=255)
                        aCATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGc                                                                                                  <  2:85907/149‑1 (MQ=255)
                         cATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCa                                                                                                 <  4:1270/149‑1 (MQ=255)
                              cTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTAcc                                                                                            >  6:289424/1‑149 (MQ=255)
                                   aTGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCAt                                                                                       >  3:307537/1‑149 (MQ=255)
                                           aTCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGTAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCAc                                                                               >  2:12819/1‑149 (MQ=255)
                                                  cgcgGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACttt                                                                        <  1:10941/149‑1 (MQ=255)
                                                   gcgGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTc                                                                       <  1:148119/149‑1 (MQ=255)
                                                    cgGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCa                                                                      >  8:155862/1‑149 (MQ=255)
                                                     gggCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTAAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAg                                                                     <  1:674/149‑1 (MQ=255)
                                                              ccGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCTCCGACTTTCAGCTCGGTTTc                                                            >  8:83333/1‑149 (MQ=255)
                                                                gcgGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCg                                                           >  5:135744/1‑148 (MQ=255)
                                                                gcgGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATCGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCg                                                           >  4:37559/1‑148 (MQ=255)
                                                                             gCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCtt                                             <  4:244087/149‑1 (MQ=255)
                                                                             gCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCtt                                             <  2:180283/149‑1 (MQ=255)
                                                                                  cAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAgc                                         <  7:155862/148‑1 (MQ=255)
                                                                                  cAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAgc                                         <  2:112121/148‑1 (MQ=255)
                                                                                   aGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAgcgc                                       <  3:258116/149‑1 (MQ=255)
                                                                                    gggTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGTAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGcc                                      <  1:12819/149‑1 (MQ=255)
                                                                                         aGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCtt                                             <  4:291212/137‑1 (MQ=255)
                                                                                         aGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCtt                                             >  3:291212/1‑137 (MQ=255)
                                                                                          ggCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACAcc                                 >  3:212152/1‑148 (MQ=255)
                                                                                           gCCGGCGATTTCCCTGGAGGTCAGCTGGTCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCa                               >  4:3394/1‑149 (MQ=255)
                                                                                                cGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCATGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCgg                          <  4:64624/149‑1 (MQ=255)
                                                                                                          ggAGGTCAGCTGGCCACCGCGGAATGTTTGCGACAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTAcc                                                                                            >  2:295970/1‑73 (MQ=255)
                                                                                                          ggAGGTCAGCTGGCCACCGCGGAATGTTTGCGACAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTAcc                                                                                            >  2:300418/1‑73 (MQ=255)
                                                                                                          ggAGGTCAGCTGGCCACCGCGGAATGTTTGCGACAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTAcc                                                                                            <  1:300418/73‑1 (MQ=255)
                                                                                                          ggAGGTCAGCTGGCCACCGCGGAATGTTTGCGACAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTAcc                                                                                            <  1:295970/73‑1 (MQ=255)
                                                                                                           gAGGTCAGCTGGTCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCa                 <  3:3394/147‑1 (MQ=255)
                                                                                                                     ggCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGggcgg     >  2:152048/1‑149 (MQ=255)
                                                                                                                       ccACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGGGCGGcc   <  1:152048/149‑1 (MQ=255)
                                                                                                                        cACCGCGGAATGTTTGCGGCAGTGAGCGGCCTTCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTATCAGCGCGGTTTAGGACAGCTTTGCCTTCAGAGCCACACCCAGCCCGCCAAATTCATCGGCCGGGCGGCCg  >  3:217921/1‑149 (MQ=37)
                                                                                                                        cACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGGGCGGcc   >  6:291967/1‑148 (MQ=255)
                                                                                                                        cACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGGGCGGCCg  >  3:12231/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                  
TGAAGGCGGCTTTACCGTTGAGCGACATGTCTTCCATGCTGGCATCGTTGCGCGGGCTGTTGCCGCGGAACTGGCCGGCGTACAGGGTCAGGCCGGCGATTTCCCTGGAGGTCAGCTGGCCACCGCGGAATGTTTGCGGCAGTGAGCGGCCATCGTCCGAACGCAGGATTGGCAGTACCGGCATCCATTCACCGACTTTCAGCTCGGTTTCGGACAGCTTGGCCTTCAGCGCCACACCCAGCCGGCCAAAGTCATCGGCCGGGCGGCCG  >  NC_002947/1575880‑1576148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑