Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,576,757 A→T 7.7% intergenic (‑277/+36) galP‑II ← / ← ttgC porin‑like protein/efflux pump outer membrane protein TtgC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,576,7570AT 7.7% 150.1 / 3.1 52intergenic (‑277/+36)galP‑II/ttgCporin‑like protein/efflux pump outer membrane protein TtgC
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base T (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

GCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGA  >  NC_002947/1576619‑1576900
                                                                                                                                           |                                                                                                                                               
gcAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTggg                                                                                                                                       >  5:161288/1‑149 (MQ=255)
  aaaCGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCt                                                                                                                                     >  8:42643/1‑149 (MQ=255)
     cggaacggGGCGAAAATCGCACGGAATGGTCCCGGTTGTTAATAACTCTTCCGGGGCCGCCGCTTCGCGGGTAAACCCGCTCCTACGGCGGCGTGCGCCCACCCGGGACAGCGCAGTAAAAACAAAGCCCACCCTTATCGGTGGGCttt                                                                                                                                   <  1:225230/149‑1 (MQ=25)
        aacggGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGc                                                                                                                               <  7:42643/149‑1 (MQ=255)
           gtgtcGATAATCGAACGGTATGTTGGGGTTGGTTAATAACTGTTCTGTGGCGGCCTCTTCGCGGGTAAAGCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCttt                                                                                                                            <  6:60709/145‑1 (MQ=38)
              gCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGc                                                                                                                         <  4:288722/149‑1 (MQ=255)
                         aCGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACACGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGa                                                                                                              >  5:172213/1‑149 (MQ=255)
                                gTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGc                                                                                                         >  7:117873/1‑147 (MQ=255)
                                gTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGc                                                                                                         <  8:117873/147‑1 (MQ=255)
                                    cGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCg                                                                                                   <  4:108058/149‑1 (MQ=255)
                                          gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCg                                                                                                   <  4:123198/143‑1 (MQ=255)
                                          gttAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCg                                                                                                   >  3:123198/1‑143 (MQ=255)
                                                   tcttctGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCAc                                                                                         <  1:113650/144‑1 (MQ=255)
                                                   tcttctGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCAc                                                                                         >  2:113650/1‑144 (MQ=255)
                                                            gcggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGcc                                                                                                        >  8:197592/1‑120 (MQ=255)
                                                            gcggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGcc                                                                                                        <  7:197592/120‑1 (MQ=255)
                                                            gcggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCAcc                                                                                        <  1:139194/136‑1 (MQ=255)
                                                            gcggcCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCAcc                                                                                        >  2:139194/1‑136 (MQ=255)
                                                                          ggTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTc                                                             >  1:8734/1‑149 (MQ=255)
                                                                              aaCCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                          <  4:161855/148‑1 (MQ=255)
                                                                                cccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                                       >  2:6205/1‑149 (MQ=255)
                                                                                cccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTACTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCctgct                                                                              <  3:169536/126‑1 (MQ=255)
                                                                                cccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTACTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCctgct                                                                              >  4:169536/1‑126 (MQ=255)
                                                                                 ccGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCg                                                       >  1:198491/1‑148 (MQ=255)
                                                                                  cGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTa                                                     <  3:191360/149‑1 (MQ=255)
                                                                                   gCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTAc                                                    <  1:6205/149‑1 (MQ=255)
                                                                                    cTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTACTCGTAc                                                    >  1:136213/1‑148 (MQ=255)
                                                                                       cTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCa                                                  <  6:172213/147‑1 (MQ=255)
                                                                                       cTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGc                                                >  5:22447/1‑149 (MQ=255)
                                                                                          cgacgGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATACGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGcc                                               <  2:250657/147‑1 (MQ=255)
                                                                                           gacgGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            >  3:209964/1‑149 (MQ=255)
                                                                                                    cgcCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAgcgc                                   <  2:198491/149‑1 (MQ=255)
                                                                                                     gcCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCTACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCg                                           <  1:242701/140‑1 (MQ=255)
                                                                                                     gcCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCg                                           >  2:242701/1‑140 (MQ=255)
                                                                                                     gcCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGcc                                  <  6:161288/149‑1 (MQ=255)
                                                                                                             ggCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAg                          >  3:132751/1‑149 (MQ=255)
                                                                                                                acaG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTAGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGtt                       >  6:275534/1‑149 (MQ=255)
                                                                                                                  aG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGa                     >  8:82476/1‑149 (MQ=255)
                                                                                                                     gCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                          <  8:10502/110‑1 (MQ=255)
                                                                                                                     gCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGACGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGc                                                          >  7:10502/1‑110 (MQ=255)
                                                                                                                      cAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTACTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCt                  <  2:136213/149‑1 (MQ=255)
                                                                                                                         tAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGc               >  6:279589/1‑149 (MQ=255)
                                                                                                                           aaaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTg             <  4:209964/149‑1 (MQ=255)
                                                                                                                            aaaCAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTgg            >  6:268979/1‑149 (MQ=255)
                                                                                                                              agaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGt           >  6:42216/3‑148 (MQ=255)
                                                                                                                               gaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            <  6:147273/113‑1 (MQ=255)
                                                                                                                               gaaaGCCCACCCTTATCGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            >  5:147273/2‑114 (MQ=255)
                                                                                                                                aaaGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAg        <  2:8734/149‑1 (MQ=255)
                                                                                                                                  aGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTTGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCt      <  5:275534/149‑1 (MQ=255)
                                                                                                                                      cACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGa  >  7:11815/1‑149 (MQ=255)
                                                                                                                                       aCCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            >  2:115855/1‑106 (MQ=255)
                                                                                                                                       aCCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCAcc                                            <  1:115855/106‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
GCAAACGGAACGGGGCGATAATCGAACGGAATGTTCCGGTTGGTTAATAACTCTTCTGGGGCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCTTGCGCCCAGCCGGCACAG‑GCAGTAAAAACAAAGCCCACCGATAAGGGTGGGCTTTCTGCTTTTGCAACAAGGCCGATCAGCCTTTCGGTGTTTCCACCGATGCCTGCTGGTTGGCCTGCCCGGTCTGCTCGTACCAGCCACCGCCAAGCGCCTTGTACAGGTTGACCTCGCTGGTCAGCTGCGA  >  NC_002947/1576619‑1576900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑