Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,591,987 T→G 14.3% intergenic (‑94/‑109) PP_1395 ← / → PP_1396 AraC family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,591,9870TG14.3% 117.1 / 15.5 49intergenic (‑94/‑109)PP_1395/PP_1396AraC family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base G (2/5);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

CAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTG  >  NC_002947/1591840‑1592126
                                                                                                                                                   |                                                                                                                                           
cAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTg                                                                                                                                            <  4:259120/149‑1 (MQ=255)
cAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTg                                                                                                                                            <  4:254809/149‑1 (MQ=255)
      cAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGa                                                                                                                                       <  7:110879/148‑1 (MQ=255)
       aGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATc                                                                                                                                     <  1:36909/149‑1 (MQ=255)
       aGGCGCTGCAGGTCCAGGGGCGGTGCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATc                                                                                                                                     <  1:309612/149‑1 (MQ=39)
        ggCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCa                                                                                                                                    <  1:245078/149‑1 (MQ=255)
         gcgcTGCAGTGCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTCGATTGCCTGATCCTGCAGCGCGGCAGGATCAg                                                                                                                                   <  1:324429/149‑1 (MQ=255)
               cAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAgg                                                                                                                                  <  4:132885/144‑1 (MQ=255)
               cAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCAGCGCGGCAGGATCAgg                                                                                                                                  >  3:132885/1‑144 (MQ=255)
                     cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTGTGCTGACCGACGCAGCTTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGc                                                                                                                                           <  8:16064/129‑1 (MQ=255)
                     cAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATg                                                                                                                       >  5:225880/1‑149 (MQ=255)
                     cAGGGTCGGTTCGGTCCACGTGGCGGTGGTCATGATCGCTTTCCTCTGCTCACAGACCCAGCATAGGTTTGGGCAAACACGGGCGCTAGTCGAAAGCTGCATGAGGATTGCATGATCCTGCCGCGCTGc                                                                                                                                           >  7:16064/1‑129 (MQ=255)
                       gggTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCa                                                                                                                     <  6:77247/149‑1 (MQ=255)
                            ggttcggtGGACGTGGCGGTGGTCATGCTGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAAt                                                                                                                <  7:146092/149‑1 (MQ=255)
                            ggttcggtGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAAt                                                                                                                >  1:306839/1‑149 (MQ=255)
                            ggttcggcGGACGTGGCGGTGGTCTTGATGGCTTTCCTCTGCTGACCGACGCAGCATTGGTATGGGCAAAGACGGGCGCTAGCCGAAAGCTGCATCTGGATTGCCTGATCCTGCCGCGCTGCAGGATCTGGCAATCGACATGCAGTAAt                                                                                                                <  3:169018/149‑1 (MQ=255)
                                      aCGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgc                                                                                                      <  6:225880/149‑1 (MQ=255)
                                           gCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgcgccg                                                                                                  <  8:26492/148‑1 (MQ=255)
                                             ggtggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgcgccgcgc                                                                                               >  3:201713/1‑149 (MQ=255)
                                             ggtggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATCGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAAcgcgccgcgc                                                                                               >  2:89164/1‑149 (MQ=255)
                                               tggtCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCgg                                                                                             <  4:201713/149‑1 (MQ=255)
                                                   cATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGt                                                                                           >  6:113841/1‑147 (MQ=255)
                                                    atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAg                                                                                        >  1:85575/1‑149 (MQ=255)
                                                    atgatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAg                                                                                        >  4:268249/1‑149 (MQ=255)
                                                    atgatgGCTTTCCTCAGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGa                                                                                         <  3:226929/148‑1 (MQ=255)
                                                      gatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGc                                                                                                                                           >  7:293835/1‑96 (MQ=255)
                                                      gatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGc                                                                                                                                           <  8:293835/96‑1 (MQ=255)
                                                      gatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAg                                                                                        >  6:260798/1‑147 (MQ=255)
                                                      gatgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAg                                                                                        <  5:260798/147‑1 (MQ=255)
                                                        tgGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGcc                                                                                    >  1:102099/1‑149 (MQ=255)
                                                                ctctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTg                                                                            <  6:307720/149‑1 (MQ=255)
                                                                 tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTgg                                                                           >  2:65451/1‑149 (MQ=255)
                                                                 tctGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTgg                                                                           >  2:70060/1‑149 (MQ=255)
                                                                    gctgACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAg                                                                        >  1:293599/1‑149 (MQ=255)
                                                                               cagcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtc                                                             <  5:113841/149‑1 (MQ=255)
                                                                                agcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                            >  7:11694/1‑149 (MQ=255)
                                                                                agcaTAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTtcc                                                            >  4:68029/1‑149 (MQ=255)
                                                                                       gTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATCGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGcc                                                     <  1:89164/149‑1 (MQ=255)
                                                                                               aaaGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGAAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCaa                                             <  2:306839/149‑1 (MQ=255)
                                                                                                aaGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAg                                            <  1:70060/149‑1 (MQ=255)
                                                                                                aaGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAg                                            <  1:65451/149‑1 (MQ=255)
                                                                                                             gTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAAt                               >  5:14347/1‑149 (MQ=255)
                                                                                                                 aaaGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGAcc                           >  8:150395/1‑149 (MQ=255)
                                                                                                                  aaGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGAccc                          <  2:85575/149‑1 (MQ=255)
                                                                                                                        cATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTaaa                     >  4:208777/1‑148 (MQ=255)
                                                                                                                                   ccTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCCGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacc         >  3:66094/1‑149 (MQ=255)
                                                                                                                                    cTGATCCTGCAGCGCGGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTcacc         >  2:325988/1‑148 (MQ=255)
                                                                                                                                          cTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCtt   <  8:11694/148‑1 (MQ=255)
                                                                                                                                          cTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTg  >  6:178185/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CAGCTCCAGGCGCTGCAGTTCCAGGGTCGGTTCGGTGGACGTGGCGGTGGTCATGATGGCTTTCCTCTGCTGACCGACGCAGCATAGGTTTGGGCAAAGACGGGCGCTAGTCGAAAGCTGCATGTGGATTGCCTGATCCTGCCGCGCTGCAGGATCAGGCAATCGACATGCAGTAATGGCCTAACGCGCCGCGCGGGTGAGCGCCTAACCTTGGCAGCCTGGCTTTTCCGTCCGCCTGCTTCAAGGAGATTGTGCAATGACCCTTAAACAACCGGTCACCCACCTTG  >  NC_002947/1591840‑1592126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑